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Species: Mycobacterium tuberculosis
Genetic Insert: DDC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5000; Vector Backbone:pCDNA3.1 (+) Zeo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24820872
Proper citation: RRID:Addgene_51529 Copy
Genetic Insert: apramycin antibiotic resistance gene
Vector Backbone Description: Backbone Marker:Takara; Backbone Size:2600; Vector Backbone:pMD18-T Simple; Vector Types:genetic modification; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24747889
Proper citation: RRID:Addgene_51655 Copy
Vector Backbone Description: Backbone Marker:Stillman Lab, Addgene plasmid # 51661; Vector Backbone:HO-Poly-HO; Vector Types:Yeast Expression, yeast integration; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11410682
Proper citation: RRID:Addgene_51662 Copy
Vector Backbone Description: Backbone Marker:Stillman Lab, Addgene plasmid # 51661; Vector Backbone:HO-Poly-HO; Vector Types:Yeast Expression, yeast integration; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11410682
Proper citation: RRID:Addgene_51664 Copy
Species: Mus musculus
Genetic Insert: Aim2
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6264; Vector Backbone:pMSCVpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19158676
Proper citation: RRID:Addgene_51667 Copy
Genetic Insert: Bxb1 integrase
Vector Backbone Description: Vector Backbone:pCMVSPORTbetaGal; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24304893
Proper citation: RRID:Addgene_51552 Copy
Species: Synthetic
Genetic Insert: superfolder GFP
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23893733
Comments: The sfGFP in this plasmid contains Y148C and K214I mutations that do not affect the function of the plasmid.
Proper citation: RRID:Addgene_51559 Copy
Species: Homo sapiens
Genetic Insert: CD4
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24036351
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
The CD4 insert in this plasmid contains N64I and K385E mutations when compared to GenBank reference sequence NP_038688.2.
Proper citation: RRID:Addgene_51604 Copy
Species: Arabidopsis thaliana
Genetic Insert: PhyB
Vector Backbone Description: Backbone Size:7236; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23761071
Comments: There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.
Proper citation: RRID:Addgene_51571 Copy
Species: Homo sapiens
Genetic Insert: FKBP
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pmCherry-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24711504
Proper citation: RRID:Addgene_51614 Copy
Species: Homo sapiens
Genetic Insert: FRB
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4902; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24711504
Proper citation: RRID:Addgene_51613 Copy
Species: Homo sapiens
Genetic Insert: TTC7B
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4979; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24711504
Proper citation: RRID:Addgene_51615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Size:6300; Vector Backbone:pKD46; Vector Types:Bacterial Expression, Red recombinase and I-SceI endonuclease; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24747889
Proper citation: RRID:Addgene_51628 Copy
Species: Homo sapiens
Genetic Insert: Pontin
Vector Backbone Description: Backbone Marker:Invitrogen/LIfe Technologies; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18358808
Proper citation: RRID:Addgene_51635 Copy
Species: Synthetic
Genetic Insert: Codon-optimized Cas9 D10A
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24491566
Comments: This plasmid vector was constructed by mutation PCR of pCAG-T3-hCAS9-pA plasmid vector (Addgene plasmid 48625) using the primers (Forward primer, 5’-AGTACTCCATTGGGCTCGccATCGGCACAAAC, and Reverse primer, 5’-CGAGCCCAATGGAGTACTTCTTGTCGGCTGC).
For the in vitro synthesis of CAS9 D10A mRNAs, the vector was linearized by SphI and in-vitro transcribed using T3-RNA-polymerase (Promega) in the presence of m7G(5′)ppp(5′)G to synthesize capped RNA.
The Tbpl1 3′UTR 95 nucleotide polyA tail at the 3' end of the insert allows in vitro transcribed mRNA from this plasmid to be used directly for microinjection into animal embryos or oocytes without additional polyadenylation treatment.
The CAG promoter in this plasmid also allows it to be used as a CAS9-expression vector.
Proper citation: RRID:Addgene_51638 Copy
Species: Homo sapiens
Genetic Insert: TERT
Vector Backbone Description: Backbone Marker:Invitrogen/LIfe Technologies; Backbone Size:5500; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18358808
Comments: Addgene full plasmid sequencing noted a conserved E286D substitution in hTert.
Proper citation: RRID:Addgene_51637 Copy
Species: Mus musculus
Genetic Insert: Sema4D
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24036351
Comments: Please note that the sequence of the myc epitope tag in this plasmid is MQKLISEEDLL, which differs from the standard EQKLISEEDLL.
The Sema4D insert in this plasmid does not contain amino acids 1-50 and contains a L745P mutation when compared to GenBank reference sequence NP_542764.2
Proper citation: RRID:Addgene_51599 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51778 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51775 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51774 Copy
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