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Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:YTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172730 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:YTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172729 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:YTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172728 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: SARS-CoV-2 S HexaPro-D614G
Vector Backbone Description: Vector Backbone:YTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34919820
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.03.30.437622v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_172727 Copy
Species: Synthetic
Genetic Insert: pmCDA1-T7RNAP
Vector Backbone Description: Backbone Size:5340; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33406230
Proper citation: RRID:Addgene_173124 Copy
Species: Shewanella oneidensis, Bacillus subtilis
Genetic Insert: SO_4388(REC)-PsdR(DBD) 137
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33972436
Proper citation: RRID:Addgene_173171 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: ERV1
Vector Backbone Description: Vector Backbone:pDisCoTune; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34405535
Proper citation: RRID:Addgene_173713 Copy
Vector Backbone Description: Backbone Marker:Nigel P Minton; Backbone Size:6768; Vector Backbone:pMTLSC-7215; Vector Types:E. coli - C. difficile shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34131082
Proper citation: RRID:Addgene_167281 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, R204C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167620 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, H20A, N208C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167625 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, N208C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167624 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, H20A, R212C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167629 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, H20A, A209C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167627 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, Q160C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167616 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, H20A, M201C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167619 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, H20A, R214C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167631 Copy
Species: Escherichia coli
Genetic Insert: tonB C18G, R214C
Vector Backbone Description: Vector Backbone:pPro33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Parent plasmid is pKP1362 in this collection. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167630 Copy
Species: Escherichia coli
Genetic Insert: tonB fs
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: The frame-shift of the TonB amphipathic helix was produced by deletion of bp 607 (G) and insertion of a new bp (T) following position 636 of the subsequent tonB open reading frame. Please note that some Genbank listings and Snapgene's ORF recognition software may add additional amino acids to the TonB ORF. Postle and Skare, 1988, PMID: 2839513 demonstrated that the first two Met residues of the open reading frame are not translated and that TonB starts with the third ATG codon. The mutations listed above are numbered based on the functional starting codon.
Proper citation: RRID:Addgene_167593 Copy
Genetic Insert: chloramphenicol acetyl transferase
Vector Backbone Description: Backbone Marker:Jan-Peter van Pijkeren; Backbone Size:9747; Vector Backbone:pVPL3004; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34232061
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.09.01.278044v2 for BioRxiv preprint.
Proper citation: RRID:Addgene_167547 Copy
Species: Escherichia coli
Genetic Insert: cba-his6, A121C, Y254C
Vector Backbone Description: Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: The parent plasmid is pKP864 in this collection. Colicin B protein expression can be induced by mitomycin C treatment in a recA+ strain, i.e. not DH5alpha (PMID: 17628143).
Proper citation: RRID:Addgene_167660 Copy
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