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Species: Mus musculus
Genetic Insert: sgRNA M5, sgRNA M7, sgRNA M9
Vector Backbone Description: Vector Backbone:pT2K-CAGGS-IRES-CFP; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29880921
Proper citation: RRID:Addgene_114729 Copy
Genetic Insert: LacZ
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4895; Vector Backbone:pGL3; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32427827
Proper citation: RRID:Addgene_114670 Copy
Species: Mus musculus
Genetic Insert: Otopetrin 1
Vector Backbone Description: Backbone Size:3019; Vector Backbone:pGEMHE; Vector Types:For generating the mRNA, which can highly express in Xenopus laevis oocytes; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29371428
Proper citation: RRID:Addgene_114674 Copy
Genetic Insert: Gateway ccdB reading frame cassette
Vector Backbone Description: Backbone Size:11600; Vector Backbone:RCASBP-Y; Vector Types:Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:11759842
Proper citation: RRID:Addgene_11480 Copy
Species: Homo sapiens
Genetic Insert: mdm2
Vector Backbone Description: Backbone Marker:GE-Amersham; Backbone Size:4900; Vector Backbone:pGEX4T1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10722742
Proper citation: RRID:Addgene_11492 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:5703; Vector Backbone:pLA1 (pRS313:GAL1-10); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1149 Copy
Species: Homo sapiens
Genetic Insert: Frataxin
Vector Backbone Description: Backbone Marker:pBABE-hygro is available at Addgene; Backbone Size:5558; Vector Backbone:pBABE hygro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11035806
Comments: This plasmid was cloned before there was any consensus sequence for frataxin. There are 2 mutations with the currently available frataxin sequence - L140P R165C. The effect of these mutations is unknown.
Proper citation: RRID:Addgene_11504 Copy
Genetic Insert: Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4045; Vector Backbone:pRL-TK; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16483934
Comments: The plasmid constructed by PCR cloning HCV IRES into the NheI site of pRL-TKx6 and inserting the Firefly luciferase ORF from pGL3 into the resulting vectorl.
Proper citation: RRID:Addgene_11510 Copy
Species: Homo sapiens
Genetic Insert: huntingtin, exon 1, 46Q glutamines
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5900; Vector Backbone:pET-32a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12193654
Proper citation: RRID:Addgene_11515 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6087; Vector Backbone:Piggybac; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30793977
Comments: Please visit https://www.biorxiv.org/content/early/2018/08/20/396390 for bioRxiv preprint.
Proper citation: RRID:Addgene_115144 Copy
Species: Synthetic
Genetic Insert: human codon optimized Mb3Cas12a
Vector Backbone Description: Backbone Marker:Cepko lab (Addgene Plasmid #11179); Vector Backbone:pCAG (Addgene #11179); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115142 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA1 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115136 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA2 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115138 Copy
Species: Synthetic
Genetic Insert: human codon optimized AcrVA3.1 anti-CRISPR protein
Vector Backbone Description: Backbone Marker:Joung lab (Addgene Plasmid #43861); Vector Backbone:JDS246 (Plasmid #43861); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30190308
Proper citation: RRID:Addgene_115139 Copy
Species: Arabidopsis thaliana
Genetic Insert: AT5G46330
Vector Backbone Description: Backbone Marker:Chris Garcia (Addgene plasmid # 47032); Vector Backbone:pECIA2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29320478
Comments: Plasmid for secreted expression in Drosophila cell culture by induction via CuSO4. Insert is an extracellular domain of indicated LRR-RK, C-terminally tagged with Fc, V5 and hexahistidine tags. Insert Protein Sequence: KQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKR
Proper citation: RRID:Addgene_115120 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSP104
Vector Backbone Description: Backbone Size:5703; Vector Backbone:pLA1 (pRS313:GAL1-10); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14978213
Proper citation: RRID:Addgene_1151 Copy
Species: Mus musculus
Genetic Insert: RIPK1
Vector Backbone Description: Vector Backbone:pAAV2; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28842570
Proper citation: RRID:Addgene_115341 Copy
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8724; Vector Backbone:pDest-590; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11519 Copy
Genetic Insert: None
Vector Backbone Description: Backbone Marker:PEL; Backbone Size:8266; Vector Backbone:pDest-560; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_11518 Copy
Species: Mus musculus
Genetic Insert: shRNA targeting Plxnb1
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pSuper; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29981480
Proper citation: RRID:Addgene_115174 Copy
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