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Species: Vibrio harveyi
Genetic Insert: LuxT
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51183 Copy
Vector Backbone Description: Backbone Marker:R.D.Gietz (University of Manitoba); Vector Backbone:YEplac195; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9671822
Comments: Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.
Proper citation: RRID:Addgene_51229 Copy
Vector Backbone Description: Backbone Marker:R.D.Gietz (University of Manitoba); Vector Backbone:YEplac181; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9671822
Comments: Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.
Proper citation: RRID:Addgene_51225 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid 51225; Vector Backbone:pBEVY-GL; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9671822
Comments: Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.
Proper citation: RRID:Addgene_51226 Copy
Species: Azoarcus evanssi
Genetic Insert: PaaR
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51193 Copy
Species: Terrabacter sp. DBF63
Genetic Insert: orfL6
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51192 Copy
Vector Backbone Description: Backbone Marker:R.D.Gietz (University of Manitoba); Vector Backbone:YEplac112; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9671822
Comments: Please note that there are minor discrepancies between Addgene's quality control sequence and depositor's reference sequence. The changes are in cloning junctions and should not affect plasmid function.
Proper citation: RRID:Addgene_51231 Copy
Species: Streptomyces coelicolor
Genetic Insert: PqrA
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51195 Copy
Species: Synthetic
Genetic Insert: 3xFLAG-dCas9
Vector Backbone Description: Backbone Marker:Hodaka Fujii; Backbone Size:5791; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25051498
Comments: The coding sequence of 3xFLAG-dCas9 can be cleaved with Pac I and Not I.
Construction strategy of gRNA retroviral vectors
1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I).
2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors.
Vectors & Sites of insertion
pSIR-neo (Addgene #51128): eg. [Xho I + Hind III]
pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I
pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I
pSIR-hCD2 (Addgene #51143): eg. EcoR I
Proper citation: RRID:Addgene_51240 Copy
Species: Mus musculus
Genetic Insert: Gli3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51247 Copy
Species: Mus musculus
Genetic Insert: Gli3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51246 Copy
Species: Mus musculus
Genetic Insert: Gli3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pEF5-FRT-DEST; Vector Types:Mammalian Expression, Flpin system; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24373970
Proper citation: RRID:Addgene_51248 Copy
Species: Homo sapiens
Genetic Insert: Lin28B
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Comments: N-terminal deletion (70 amino acids)
Proper citation: RRID:Addgene_51374 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7a-1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51377 Copy
Species: Homo sapiens
Genetic Insert: pri-miR-29b-1~29a
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079265
Comments: Point mutations: insertion (1115bp), deletion (A47, T666) (Unnecessary region for miRNA biogenesis)
Proper citation: RRID:Addgene_51376 Copy
Species: Homo sapiens
Genetic Insert: Lin28B
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51373 Copy
Species: Homo sapiens
Genetic Insert: Lin28A
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Comments: H147A, H169A
Proper citation: RRID:Addgene_51372 Copy
Species: Gallus gallus
Genetic Insert: DCTN1
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pGW1-CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10525538
Proper citation: RRID:Addgene_51412 Copy
Species: Homo sapiens
Genetic Insert: pri-let-7b
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18951094
Proper citation: RRID:Addgene_51378 Copy
Species: Gallus gallus
Genetic Insert: Dynamitin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:6300; Vector Backbone:pCDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10525538
Proper citation: RRID:Addgene_51419 Copy
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