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Species: Mus musculus
Genetic Insert: IgG3 F(ab')2
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4549; Vector Backbone:pFUSEss-CHIg-mG3 (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:29875771
Proper citation: RRID:Addgene_105860 Copy
Species: Mus musculus
Genetic Insert: IgG1 heavy chain
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4474; Vector Backbone:pFUSEss-CHIg-mG1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:29875771
Proper citation: RRID:Addgene_105849 Copy
Genetic Insert: PGL3 luciferase siRNA
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15119963
Comments: Inhibits the expression of PGL3 luciferase. Opposing human H1 and human U6 Pol III promoters drive expression of siRNAs
See article and author's map for more information. In the sequence link, the PGL3 luciferase siRNA is lower case.
Addgene QC Sanger sequencing did not identify BsmBI sites adjacent to the siRNA sequence.
Proper citation: RRID:Addgene_15099 Copy
Vector Backbone Description: Backbone Marker:Moon Lab; Backbone Size:2167; Vector Backbone:pHIPPY; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:15119963
Comments: This construct is a blunt BsmB1 site version, for use as a NEGATIVE CONTROL for pHippy targetting of siRNAs in mammalian cells.
See article and author's map for more information.
Proper citation: RRID:Addgene_15098 Copy
Species: Mus musculus
Genetic Insert: Intercellular Adhesion Molecule 1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:4179; Vector Backbone:pFUSE; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32783949
Comments: Depositor confirms P66S and L234P mutations does not affect plasmid function.
Proper citation: RRID:Addgene_156462 Copy
Species: Synthetic
Genetic Insert: pMD2.G(spei/spei)&12x601_tetO
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR™BluntII-TOPO®; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31543265
Comments: High copy plasmid (pUC-based, but medium yield)
Proper citation: RRID:Addgene_157785 Copy
Species: Streptococcus thermophilus
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:2686; Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:33113377
Comments: zeocin was cloned from the pCR2.1 topo gene from the invitrogen blunt topo plasmid
Proper citation: RRID:Addgene_160903 Copy
Species: Homo sapiens
Genetic Insert: ACE2
Vector Backbone Description: Vector Backbone:pFUSE; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:33093202
Comments: Please visit https://doi.org/10.1101/2020.07.31.231746 for bioRxiv preprint.
Proper citation: RRID:Addgene_159762 Copy
Species: wheat
Genetic Insert: wheat GRF4-GIF1 chimera
Vector Backbone Description: Backbone Size:2076; Vector Backbone:pDONR-zeo; Vector Types:Entry clon; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:33046875
Proper citation: RRID:Addgene_160392 Copy
Species: Vitis
Genetic Insert: Vitis miR396-resistant GRF4-GIF1 chimera
Vector Backbone Description: Backbone Size:2076; Vector Backbone:pDONR-zeo; Vector Types:Entry clon; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:33046875
Proper citation: RRID:Addgene_160395 Copy
Species: Maristella sp. SVU
Genetic Insert: luciferase
Vector Backbone Description: Backbone Marker:Thermo Fisher; Backbone Size:3600; Vector Backbone:pPICZ-alphaC; Vector Types:Yeast Expression, Luciferase; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:33031624
Comments: Please note: Plasmid contains a 39bp deletion that removes the N-terminal amino acids AQDCYEFTLEKRE from the luciferase compared to the depositor's insert sequence. It is not known how this discrepancy affects the plasmid function, though the plasmid was functional in the depositor's experiments.
Proper citation: RRID:Addgene_160426 Copy
Species: Other
Genetic Insert: Sth1 sgRNA scaffold
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31992616
Comments: insert sizes: 171 bp and 3366 bp
Proper citation: RRID:Addgene_158719 Copy
Species: Other
Genetic Insert: Sth1 sgRNA scaffold
Vector Backbone Description: Vector Backbone:custom design; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31992616
Comments: insert sizes: 171 bp and 3366 bp
Proper citation: RRID:Addgene_158720 Copy
Species: Mus musculus
Genetic Insert: Kir3.2
Vector Backbone Description: Vector Backbone:pPICZ; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:32372643
Proper citation: RRID:Addgene_140745 Copy
Species: Other
Genetic Insert: loxp-DsRed-loxp-eGFP
Vector Backbone Description: Backbone Marker:Eric Campeau & Paul Kaufman (Addgene plasmid # 17294); Vector Backbone:pLenti CMV/TO Zeo DEST (644-1) ; Vector Types:Lentiviral; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:31649238
Comments: loxp-DsRed-loxp-eGFP was PCR amplified from pMSCV-loxp-DsRed-loxp-eGFP-Puro-WPRE (Addgene plasmid #32702) using primers containing Sal1 and Not1 restriction enzyme sites. The amplified PCR fragment was digested with Sal1 and Not1 and ligated into the pENTRA1 vector cut with Sal1 and Not1. The Gateway SystemTM (Invitrogen) was used to recombine the pENTR1A shuttle vector with pLenti CMV/TO Zeo DEST (644-1) (#17294, Addgene) generating lentiviral vector 1.
Proper citation: RRID:Addgene_141148 Copy
Species: Homo sapiens
Genetic Insert: KCNK4
Vector Backbone Description: Vector Backbone:pPICZ; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:25500157
Proper citation: RRID:Addgene_130672 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Green lab; Backbone Size:3301; Vector Backbone:Z1; Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:42490567
Proper citation: RRID:Addgene_135046 Copy
Species: Synthetic
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Green lab; Vector Backbone:Z1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:42490567
Proper citation: RRID:Addgene_135612 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: HMS clone ID number: ScCD00063803.
Proper citation: RRID:Addgene_133437 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: STE2
Vector Backbone Description: Vector Backbone:pPICZalpha; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin)
Comments: HMS clone ID number: ScCD00063802.
Proper citation: RRID:Addgene_133436 Copy
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