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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
JM101
 
Resource Report
Resource Website
RRID:Addgene_50349 Relevant genotype: supE, thi, Δ(lac-proAB), [F' traD36, proAB, lacIqZΔM15] E. coli None Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None 2026-08-15 01:16:05 0
JM83
 
Resource Report
Resource Website
RRID:Addgene_50348 Relevant genotype: ara, Δ(lac-proAB), rspL(+strA), ϕ80, lacZΔM15 E. coli None Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None 2026-08-15 01:16:07 0
HL 5226
 
Resource Report
Resource Website
RRID:Addgene_63667 see comments None PMID:26261213 MG1655 + lacIq intergrated at intS + ΔglmZ Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-08-15 01:18:04 0
HL 4233
 
Resource Report
Resource Website
RRID:Addgene_52944 none E.coli None PMID:25087841 Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-08-15 01:16:26 0
HL 932
 
Resource Report
Resource Website
RRID:Addgene_52940 none E.coli None PMID:25087841 Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-08-15 01:16:26 0
HL3796
 
Resource Report
Resource Website
1+ mentions
RRID:Addgene_52951 none E.coli None Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-08-15 01:16:26 2
HL4018
 
Resource Report
Resource Website
RRID:Addgene_52952 none E.coli None Vector Backbone:none; Vector Types:Synthetic Biology; Bacterial Resistance:None 2026-08-15 01:16:26 0
E. coli BW25113 ΔphoP:PcpcG2-phoP*** (CMB259)
 
Resource Report
Resource Website
RRID:Addgene_254897 Carries a single chromosomal copy of constitutively active PhoP downstream of PcpcG2 promoter inserted in nupG locus None PMID:41996246 For validation, use primers: FWD: CGTGAGCGGTAAAGTTGTTG and REV: CGGAGCTGCAAGGTGTTTATAG flanking the nupG locus to verify gene insertion. Please visit https://doi.org/10.1101/2024.11.27.625634 for bioRxiv preprint. Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None 2026-08-15 01:35:53 0
E. coli BW25113:PcpcG2-tetR (CMB247)
 
Resource Report
Resource Website
RRID:Addgene_254898 Carries a single chromosomal copy of the repressor TetR downstream of PcpcG2 promoter inserted in nupG locus None PMID:41996246 For validation, use primers: FWD: CGTGAGCGGTAAAGTTGTTG and REV: CGGAGCTGCAAGGTGTTTATAG flanking the nupG locus to verify gene insertion. Please visit https://doi.org/10.1101/2024.11.27.625634 for bioRxiv preprint. Vector Backbone:n/a; Vector Types:; Bacterial Resistance:None 2026-08-15 01:35:53 0
TB201
 
Resource Report
Resource Website
RRID:Addgene_230031 MG1655 attP21::PR-mYFP::frt None PMID:29355812 Strain Validation: Fluorescence, PCR for fluorescent marker gene. Inserted into attP21 and PCR-verified following methods described in Haldimann and Wanner, JBact 2001, PMID 11591683, using pAH68FRT-Cat as cloning and insertion plasmid. Vector Backbone:NA; Vector Types:; Bacterial Resistance:None 2026-08-15 01:31:48 0
TB205
 
Resource Report
Resource Website
RRID:Addgene_230034 MG1655 attP21::PR-mCherry::frt None PMID:28428424 Strain Validation: Fluorescence, PCR for fluorescent marker gene. Inserted into attP21 and PCR-verified following methods described in Haldimann and Wanner, JBact 2001, PMID 11591683, using pAH68FRT-Cat as cloning and insertion plasmid. TB205 is fliC+ and wrongly annotated as ∆fliC in PMID 28428424. Vector Backbone:NA; Vector Types:; Bacterial Resistance:None 2026-08-15 01:31:48 0
TB204
 
Resource Report
Resource Website
RRID:Addgene_230033 MG1655 attP21::PR-sfGFP::frt None PMID:29355812 Strain Validation: Fluorescence, PCR for fluorescent marker gene. Inserted into attP21 and PCR-verified following methods described in Haldimann and Wanner, JBact 2001, PMID 11591683, using pAH68FRT-Cat as cloning and insertion plasmid. Vector Backbone:NA; Vector Types:; Bacterial Resistance:None 2026-08-15 01:31:48 0
TB202
 
Resource Report
Resource Website
RRID:Addgene_230032 MG1655 attP21::PR-mCerulean::frt None Strain Validation: Fluorescence, PCR for fluorescent marker gene. Inserted into attP21 and PCR-verified following methods described in Haldimann and Wanner, JBact 2001, PMID 11591683, using pAH68FRT-Cat as cloning and insertion plasmid. Vector Backbone:NA; Vector Types:; Bacterial Resistance:None 2026-08-15 01:31:48 0
TB205 △trpC
 
Resource Report
Resource Website
RRID:Addgene_230038 MG1655 attP21::PR-mCherry::frt trpC::frt None PMID:32042125 Strain Validation: Fluorescence, growth in M9+glucose +/- tryptophane, PCRs with primers flanking fluorescent marker gene or deleted gene. Primers: trpC_fwd: AACGTCGCCATGTTAATGCG trpC_rev: GAACTGAGCCTGAAATTCAGG Vector Backbone:NA; Vector Types:; Bacterial Resistance:None 2026-08-15 01:31:47 0
TB204 △trpC
 
Resource Report
Resource Website
RRID:Addgene_230037 MG1655 attP21::PR-sfGFP::frt trpC::frt None PMID:32042125 Strain Validation: Fluorescence, growth in M9+glucose +/- tryptophane, PCRs with primers flanking fluorescent marker gene or deleted gene. Primers: trpC_fwd: AACGTCGCCATGTTAATGCG trpC_rev: GAACTGAGCCTGAAATTCAGG Vector Backbone:NA; Vector Types:; Bacterial Resistance:None 2026-08-15 01:31:47 0
Δrpi
 
Resource Report
Resource Website
RRID:Addgene_234837 ΔrpiAB Δedd strain None Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None 2026-08-15 01:33:27 0
TB205 △proC △hisL
 
Resource Report
Resource Website
RRID:Addgene_229552 MG1655 attP21::PR-mCherry::frt proC::frt hisL::frt n/a None PMID:40509754 Primers for sequence verification of hisL knock-out: Fwd - prEP229, gtgaggataagaggtggcga Rev - prEP230, tcctttccccgctcattcat Please visit https://doi.org/10.1101/2024.07.19.604250 for bioRxiv preprint. Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None 2026-08-15 01:33:02 0
TB204 △metB proB74
 
Resource Report
Resource Website
RRID:Addgene_229551 MG1655 attP21::PR-sfGFP::frt metB:frt proBA::proB74proA n/a None PMID:40509754 Primers for sequence verification of proB mutation: Fwd - prEP169, cgcggttatgtgaagaacgt Rev - prEP170, gtgatgtcgcgttataccgg Please visit https://doi.org/10.1101/2024.07.19.604250 for bioRxiv preprint. Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None in proB: Aspartic acid 107 mutated to asparagine (D107N) GAT->AAT 2026-08-15 01:33:02 0
TB204 △metB
 
Resource Report
Resource Website
RRID:Addgene_229549 MG1655 attP21::PR-sfGFP::frt metB:frt n/a None PMID:40509754 Primers for sequence verification of metB knock-out: Fwd - prEP213, acgatcggtctggcttagtt Rev - prEP214, tgaccgtaaacccgcatagt Please visit https://doi.org/10.1101/2024.07.19.604250 for bioRxiv preprint. Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None 2026-08-15 01:33:02 0
TB204 △hisD
 
Resource Report
Resource Website
RRID:Addgene_229548 MG1655 attP21::PR-sfGFP::frt hisD:frt n/a None PMID:40509754 Primers for sequence verification of hisD knock-out: Fwd - prEP209, ccggttttacgcctgcatat Rev - prEP210, ccgaacgcccaactattctg Please visit https://doi.org/10.1101/2024.07.19.604250 for bioRxiv preprint. Vector Backbone:n/a; Vector Types:This is a strain, not a plasmid; Bacterial Resistance:None 2026-08-15 01:33:02 0

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