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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pIN205 Resource Report Resource Website |
RRID:Addgene_255796 | NrdJ-1C - XtpS | Xenorhabdus nematophila ATCC 19061 | Spectinomycin and Streptomycin | This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTII site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN092 and pIN201, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose. | Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:36:17 | 0 | ||
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pIN207 Resource Report Resource Website |
RRID:Addgene_255798 | SspGyrBC - XtpS | Xenorhabdus nematophila ATCC 19061 | Spectinomycin and Streptomycin | This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XU site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN103 and pIN203, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol). For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains. Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose. | Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:36:21 | 0 | ||
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pWY82 Resource Report Resource Website 1+ mentions |
RRID:Addgene_65721 | Telomere | Arabidopsis thaliana | Spectinomycin and Streptomycin | PMID:17085598 | Please note that Addgene NGS found a 1.5kb deletion in the telomere repeats as compared to the original depositor sequence. The depositor has confirmed that the telomere sequence in the plasmid is inherently unstable, and that the plasmid is still functional for telomere truncation. The plasmid was also verified by restriction digest. The gel image is provided on this page under- Resource Information- Addgene Notes. | Backbone Size:9189; Vector Backbone:pTF101.1; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:18:22 | 3 | |
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pDFC(P0) Resource Report Resource Website |
RRID:Addgene_251363 | Spectinomycin and Streptomycin | PMID:41699564 | Vector Backbone:pDFC; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin | 2026-08-15 01:35:24 | 0 | ||||
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pART27::CLOMELEON Resource Report Resource Website |
RRID:Addgene_247988 | CLOMELEON (CFP-YFP fusion with ENLYFQG-linker) | Synthetic | Spectinomycin and Streptomycin | PMID:15086798 | pART27::CLOMELEON is a binary pART27 vector harbouring a NotI-fragment with a gene cassette subcloned from pART7::CLOMELEON to stably insert the CLOMELEON gene (Kuner, T. & Augustine, G.J.; PubMed: 11055428; doi: 10.1016/S0896-6273(00)00056-8) into the genome of plants by Agrobacterium-mediated gene transfer, e.g. by floral dip (Clough & Bent 1998; PubMed: 10069079; doi: 10.1007/BF00028910). See the Supplemental Document under Resource Information for more details, including annotated maps. | Backbone Marker:Andrew Gleave; Backbone Size:11612; Vector Backbone:pART27; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin and Streptomycin | CFP-variant: K26R, F64L, S65T, Y66W, N146I, M153T, V163A, N164H, H231L; YFP-variant: S65G, S72A, K79R, T203Y, H231L | 2026-08-15 01:34:45 | 0 |
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