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Species: Caenorhabditis elegans
Genetic Insert: Prps-0::mKate2:: par-6(_PB1)::unc-54 3' UTR
Vector Backbone Description: Backbone Marker:Agilent Technologies; Backbone Size:2999; Vector Backbone:pMLS257; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34849800
Comments: Please visit https://doi.org/10.1101/2021.06.01.446599 for bioRxiv preprint.
Proper citation: RRID:Addgene_173752 Copy
Species: Caenorhabditis elegans
Genetic Insert: PSEC63_yeGFP_cePUP-2_URA3_TADH1
Vector Backbone Description: Backbone Size:5702; Vector Backbone:NA; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32907940
Proper citation: RRID:Addgene_167454 Copy
Species: Caenorhabditis elegans
Genetic Insert: pgl-3
Vector Backbone Description: Vector Backbone:pMAL-c2X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171208 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171207 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171205 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pet28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171204 Copy
Species: Caenorhabditis elegans
Genetic Insert: meg-3
Vector Backbone Description: Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.10.15.340570v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171203 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-3::mNeonGreen
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37396790
Comments: C. elegans codon-optimized mNeonGreen. Use as an array marker for CRISPR or as a general co-injection marker. Very bright mNG fluorescence in body wall muscle. Inject at 5 ng/uL. Made by George Huang (Glow Worms '20).
pGLOW79 has 10 random mutations relative to the pCFJ104 backbone that do not affect plasmid function.
Proper citation: RRID:Addgene_177339 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2::mNeonGreen
Vector Backbone Description: Backbone Size:2445; Vector Backbone:N/A; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37396790
Comments: C. elegans codon-optimized mNeonGreen. Use as an array marker for CRISPR or as a general co-injection marker. Very bright mNG fluorescence in pharyngeal muscle. Inject at 2.5 ng/uL. Made by George Huang (Glow Worms '20).
pGLOW77 has 11 random mutations relative to the pCFJ90 backbone that do not affect plasmid function.
Proper citation: RRID:Addgene_177338 Copy
Species: Caenorhabditis elegans
Genetic Insert: grh-1
Vector Backbone Description: Vector Backbone:pdestMB14 (Addgene #26415); Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35013237
Proper citation: RRID:Addgene_177762 Copy
Species: Caenorhabditis elegans
Genetic Insert: wrmScarlet11^SEC^3xMyc
Vector Backbone Description: Backbone Size:3881; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Comments: The split-wrmScarlet system was developed and published by Goudeau et al. Genetics 2021. pGLOW63 is a derivative of published TagRFP-SEC vectors (Dickinson et al. Genetics 2015). It has a 3xMyc tag in place of 3xFlag and Lox2272 sites in place of LoxP. These features allow pGLOW63 to be used in a genetic background that has already been modified using a green FP-SEC vector, without conflicts between epitope tags and Lox sites. pGLOW63 must also be used in a genetic background that has wrmScarlet1-10. Made by Amelie Perez (Glow Worms '21).
Proper citation: RRID:Addgene_178877 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-2
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:3700; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_178898 Copy
Species: Caenorhabditis elegans
Genetic Insert: DAF-2
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7439; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_178796 Copy
Species: Caenorhabditis elegans
Genetic Insert: EGL-3
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7538; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24671950
Proper citation: RRID:Addgene_178791 Copy
Species: Caenorhabditis elegans
Genetic Insert: UNC-9
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8030; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179224 Copy
Species: Caenorhabditis elegans
Genetic Insert: UNC-7
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:7737; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179220 Copy
Species: Caenorhabditis elegans
Genetic Insert: UNC-9
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8291; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179247 Copy
Species: Caenorhabditis elegans
Genetic Insert: UNC-9
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:9228; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179249 Copy
Species: Caenorhabditis elegans
Genetic Insert: TWK-18
Vector Backbone Description: Backbone Marker:stratagene; Backbone Size:8031; Vector Backbone:pBSK; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22099460
Proper citation: RRID:Addgene_179250 Copy
Species: Caenorhabditis elegans
Genetic Insert: FBF-2
Vector Backbone Description: Backbone Marker:Chris Lima; Backbone Size:5633; Vector Backbone:pSMT3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30652968
Proper citation: RRID:Addgene_166976 Copy
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