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Species: Caenorhabditis elegans
Genetic Insert: CeSmg7_1-395-G80E
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23348841
Comments: ORF extracted from NCBI:NM_068632.4 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147476 Copy
Species: Caenorhabditis elegans
Genetic Insert: CeAIN2_254-400
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23172285
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147407 Copy
Species: Caenorhabditis elegans
Genetic Insert: CeAIN1-W378AW437A
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22402495
Comments: ORF extracted from NCBI:NM_078286.4 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147136 Copy
Species: Caenorhabditis elegans
Genetic Insert: CeALG1
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22402495
Comments: ORF extracted from NCBI: NM_077921.5 Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_147147 Copy
Species: Caenorhabditis elegans
Genetic Insert: linker::3xMyc for NT slot
Vector Backbone Description: Backbone Size:2362; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154319 Copy
Species: Caenorhabditis elegans
Genetic Insert: mNeonGreen^SEC^AID*::3xFlag
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pUC19 (modified); Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154312 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker for NT slot
Vector Backbone Description: Backbone Size:478; Vector Backbone:Topo-Blunt; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154311 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pJW1838; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33677541
Comments: Cloning by SapTrap
Proper citation: RRID:Addgene_154327 Copy
Species: Caenorhabditis elegans
Genetic Insert: cxTi10882 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pJW1838; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33677541
Comments: Cloning by SapTrap
Proper citation: RRID:Addgene_154329 Copy
Species: Caenorhabditis elegans
Genetic Insert: SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pMLS134; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33677541
Comments: Cloning by Q5 Site-Directed Mutagenesis
Proper citation: RRID:Addgene_154324 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker::GFP^SEC^AID*::3xFLAG
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154326 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker::GFP
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154322 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pJW1839; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33677541
Comments: Cloning by SapTrap
Proper citation: RRID:Addgene_154330 Copy
Species: Caenorhabditis elegans
Genetic Insert: 30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154333 Copy
Species: Caenorhabditis elegans
Genetic Insert: pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
Vector Backbone Description: Backbone Size:2360; Vector Backbone:pDONR221; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33677541
Proper citation: RRID:Addgene_154340 Copy
Species: Caenorhabditis elegans
Genetic Insert: Peft-3::wrmScarlet::tbb-2 UTR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32816924
Comments: Protocols, sequence information, and updates can be found at: https://github.com/phillips-lab/SLP
Proper citation: RRID:Addgene_154824 Copy
Species: Caenorhabditis elegans
Genetic Insert: synthetic guide::∆HYGR::unc-54 3' UTR
Vector Backbone Description: Backbone Size:10683; Vector Backbone:pMS4; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32816924
Comments: Additional protocol information can be found at: github.com/phillips-lab/SLP
Proper citation: RRID:Addgene_154838 Copy
Species: Caenorhabditis elegans
Genetic Insert: homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
Vector Backbone Description: Backbone Size:2470; Vector Backbone:pUC57; Vector Types:Worm Expression, Cre/Lox, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32816924
Comments: The LoxP site has a point mutation that in our hands does not noticeably impact function.
The rpl-28p::mKate2::unc-54 3'UTR transgene is flanked by restriction sites to facilitate removal and replacement of this gene.
Additional protocol information can be found at: github.com/phillips-lab/SLP
Proper citation: RRID:Addgene_154840 Copy
Species: Caenorhabditis elegans
Genetic Insert: ced-4
Vector Backbone Description: Backbone Size:3422; Vector Backbone:pSL1190; Vector Types:Bacterial Expression, Cloning vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8706125
Proper citation: RRID:Addgene_139645 Copy
Species: Caenorhabditis elegans
Genetic Insert: mScarlet (no STOP codon)
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:3519; Vector Backbone:Zero blunt TOPO; Vector Types:Worm Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31848219
Proper citation: RRID:Addgene_139034 Copy
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