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Species: Saccharomyces cerevisiae
Genetic Insert: I-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18644379
Comments: Expresses the I-SceI homing endonuclease under the control of the phage T7 promoter. The sequence is codon optimized for expression in E. coli and is derived from plasmid pSCM525 (Dr. Bernard Dujon, Institut Pasteur). The protein contains a carboxyl-terminal histidine tag for purification. The expressed protein contains the K223I mutation that results in DNA nicking activity. An appended methionine-histidine sequence is present at the amino terminus.
Proper citation: RRID:Addgene_195615 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9468518
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Also contains silent mutations that introduce ApaI, BssHII, BstEII, BsiWI, MluI and SpeI sites. Expresses PI-SceI containing a carboxyl-terminal histidine tag and a K301A mutation in the PI-SceI ORF.
Proper citation: RRID:Addgene_195611 Copy
Species: Homo sapiens
Genetic Insert: Stromal Interaction Molecule 1
Vector Backbone Description: Vector Backbone:pEX-SP-YFP-STIM1(23-685) (Plasmid #18857); Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609616
Proper citation: RRID:Addgene_186622 Copy
Species: Homo sapiens
Genetic Insert: Stromal Interaction Molecule 1
Vector Backbone Description: Vector Backbone:pLV-eGFP_Addgene#36083; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35609616
Proper citation: RRID:Addgene_186620 Copy
Species: Homo sapiens
Genetic Insert: VNp-LZ-mNeongreen-hGH
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36936078
Proper citation: RRID:Addgene_182425 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: VNp6-mNeongreen-His
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36936078
Proper citation: RRID:Addgene_182388 Copy
Species: Homo sapiens
Genetic Insert: VNp-mNeongreen-eythropoietin
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36936078
Proper citation: RRID:Addgene_182423 Copy
Species: Synthetic
Genetic Insert: mNeonGreen
Vector Backbone Description: Backbone Size:4422; Vector Backbone:TRE3g; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36198803
Proper citation: RRID:Addgene_192064 Copy
Species: Branchiostoma lanceolatum
Genetic Insert: VNp-mNeongreen-His
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36936078
Proper citation: RRID:Addgene_182390 Copy
Species: Homo sapiens
Genetic Insert: sgRNA
Vector Backbone Description: Backbone Marker:Jonathan Weissman; Backbone Size:8877; Vector Backbone:pU6-sgRNA EF1Alpha-puro-T2A-BFP (Plasmid #60955); Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36719724
Proper citation: RRID:Addgene_195501 Copy
Species: Agrobacterium radiobacter
Genetic Insert: DARPinOFF7
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36936078
Proper citation: RRID:Addgene_182396 Copy
Species: Homo sapiens
Genetic Insert: Human Reticulon 2 isoform B
Vector Backbone Description: Vector Backbone:GFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35609616
Proper citation: RRID:Addgene_186599 Copy
Species: Aequorea victoria
Genetic Insert: VNp-LZ-VenusN154
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36936078
Proper citation: RRID:Addgene_182395 Copy
Species: Agrobacterium radiobacter
Genetic Insert: VNp15-DARPinOFF7
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36936078
Proper citation: RRID:Addgene_182399 Copy
Species: Homo sapiens
Genetic Insert: AURKB
Vector Backbone Description: Backbone Marker:Sanford Simon; Backbone Size:8230; Vector Backbone:pLVX-Che-hi3; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34812139
Proper citation: RRID:Addgene_153316 Copy
Species: Homo sapiens
Genetic Insert: VNp6-stefin
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36936078
Proper citation: RRID:Addgene_182404 Copy
Species: Bos taurus
Genetic Insert: DNase I
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pRSFDUET-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36936078
Proper citation: RRID:Addgene_182408 Copy
Species: Discosoma sp
Genetic Insert: DsRed
Vector Backbone Description: Backbone Size:5071; Vector Backbone:IRESpuro2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35922507
Proper citation: RRID:Addgene_191766 Copy
Species: Discosoma sp
Genetic Insert: DsRed
Vector Backbone Description: Backbone Size:5069; Vector Backbone:IRESpuro2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35922507
Proper citation: RRID:Addgene_191765 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: PI-SceI
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET-23a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7890714
Comments: Expresses the excised intein of the VMA1 gene product including amino-terminal methionine-cysteine residues. Contains silent AvrII and RsrII sites near the borders of the N-terminal LAGLIDADG motif and silent EaeI and SnaBI sites near the borders of the C-terminal motif. Contains a D218A mutation in the first LAGLIDADG motif that inactivates the protein.
Proper citation: RRID:Addgene_195608 Copy
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