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Species: Caenorhabditis elegans
Genetic Insert: MDH-1
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34619358
Proper citation: RRID:Addgene_224145 Copy
Species: Caenorhabditis elegans
Genetic Insert: ALDO-1
Vector Backbone Description: Vector Backbone:pET303/CT-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_224157 Copy
Species: Caenorhabditis elegans
Genetic Insert: GPD-3
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB3; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_224151 Copy
Species: Caenorhabditis elegans
Genetic Insert: LGC-47
Vector Backbone Description: Backbone Marker:Anuj Sharma; Backbone Size:3481; Vector Backbone:pAS3; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38585821
Proper citation: RRID:Addgene_225923 Copy
Species: Caenorhabditis elegans
Genetic Insert: daf-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2961; Vector Backbone:pBluescript; Vector Types:Bacterial Expression, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12399591
Comments: Use HT115 bacteria for experiments--Grow O/N on LB supplemented with carbenicillin (100ug/ml) & tetracyclin (12.5ug/ml) and pick single colony for liquid culture.
Proper citation: RRID:Addgene_34834 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A2;B2;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34662 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A2;B0;C1)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34660 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B2;C0)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34653 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A1;B1;C0)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34652 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A2;B1;C0)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34655 Copy
Species: Caenorhabditis elegans
Genetic Insert: slo-1(A2;B0;C0)
Vector Backbone Description: Backbone Size:2958; Vector Backbone:pBluescript KS(+); Vector Types:Xenopus oocyte expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22049343
Comments: The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.
Proper citation: RRID:Addgene_34654 Copy
Species: Caenorhabditis elegans
Genetic Insert: peel-1
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34873 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4348 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Comments: Please note that there are TWO M13F primer binding sites. Use custom sequencing primers to verify inserts into MCS.
Proper citation: RRID:Addgene_34872 Copy
Species: Caenorhabditis elegans
Genetic Insert: cxTi10816 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34871 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi14024 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34870 Copy
Species: Caenorhabditis elegans
Genetic Insert: Pmyo-2 GFP H2B tbb-2 UTR
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34876 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi14024 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34862 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4391 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: There is a 461 bp deletion in the middle of the left recombination region. This deletion does not appear to adversely affect the ability to insert transgenes into the ttTi4391 site.
Proper citation: RRID:Addgene_34861 Copy
Species: Caenorhabditis elegans
Genetic Insert: cxTi10816 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22290181
Comments: Please note there are TWO M13F primer sites. Use custom sequencing primers to sequence final gateway products.
Proper citation: RRID:Addgene_34864 Copy
Species: Caenorhabditis elegans
Genetic Insert: ttTi4391 targeting
Vector Backbone Description: Vector Backbone:pDESTR4-R3; Vector Types:Worm targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22290181
Proper citation: RRID:Addgene_34869 Copy
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