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Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:6900; Vector Backbone:pLKO.1-puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20086175
Proper citation: RRID:Addgene_37046 Copy
Species: Homo sapiens
Genetic Insert: Dsc2
Vector Backbone Description: Backbone Marker:GE Healthcare; Vector Backbone:PGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22184201
Proper citation: RRID:Addgene_36992 Copy
Species: Bovine Papillomavirus
Genetic Insert: BPV1 E6
Vector Backbone Description: Vector Backbone:N-Tap-Flag-HA; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22547818
Proper citation: RRID:Addgene_37047 Copy
Species: Homo sapiens
Genetic Insert: TGFβRII
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21245094
Comments: Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro.
Proper citation: RRID:Addgene_37040 Copy
Species: Homo sapiens
Genetic Insert: Smad4
Vector Backbone Description: Backbone Marker:Weinberg Lab; Backbone Size:5169; Vector Backbone:pBabe-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21245094
Comments: Insert was blunt-ended and ligated into SnaBI-digested pBabe-puro vector
Proper citation: RRID:Addgene_37041 Copy
Species: Homo sapiens
Genetic Insert: MAML1-ADDAA
Vector Backbone Description: Vector Backbone:pHAGE-N-V5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22547818
Comments: MAML1-ADDAA acidic domain mutant
Proper citation: RRID:Addgene_37050 Copy
Species: Mus musculus
Genetic Insert: slug
Vector Backbone Description: Backbone Marker:Dr. Tal Kafri; Backbone Size:0; Vector Backbone:pTK380 (tetracycline inducible); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: Slug cDNA was PCR amplified from an IMAGE clone (Open Biosystems) and cloned into BamHI-XbaI sites of pTK380.
Proper citation: RRID:Addgene_36986 Copy
Species: Mus musculus
Genetic Insert: Hes1
Vector Backbone Description: Backbone Marker:Trono lab (Addgene plasmid 12257); Backbone Size:10510; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: The mouse Hes1 cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the vector backbone with mouse Hes1.
Proper citation: RRID:Addgene_36983 Copy
Species: Homo sapiens
Genetic Insert: beta-catenin deltaN90
Vector Backbone Description: Backbone Marker:I. Ben-Porath; Backbone Size:0; Vector Backbone:pLV-IRES-neo; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: The pLV-neo vector was modified from the attached vector by replacing dsRed with Neomycin resistant cassette and removing tTR-kRAB.
Addgene QC sequence analysis identified a S374G discrepancy, relative to reference sequence NP_001091679.1.
Proper citation: RRID:Addgene_36985 Copy
Species: Mus musculus
Genetic Insert: c-Myc
Vector Backbone Description: Backbone Marker:Trono lab (Addgene plasmid 12257); Backbone Size:10510; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: The mouse c-myc cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the original vector with mouse c-myc.
Proper citation: RRID:Addgene_36980 Copy
Species: Mus musculus
Genetic Insert: Klf4
Vector Backbone Description: Backbone Marker:Trono lab (Addgene plasmid 12257); Backbone Size:10510; Vector Backbone:pWPXL; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: The mouse Klf4 cDNA was cloned between MluI and SpeI sites (blunted) in pWPXL. This resulted in replacement of the EGFP in the vector backbone with mouse Klf4.
Proper citation: RRID:Addgene_36981 Copy
Species: Homo sapiens
Genetic Insert: Rac1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4750; Vector Backbone:pRK5-myc; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23045281
Proper citation: RRID:Addgene_37030 Copy
Species: Mus musculus
Genetic Insert: Snail
Vector Backbone Description: Backbone Marker:Dr. Tal Kafri; Backbone Size:0; Vector Backbone:pTK380 (tetracycline inducible); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: Snail cDNA was PCR amplified from IMAGE clone and cloned into BamHI-XbaI sites of pTK380.
Proper citation: RRID:Addgene_36976 Copy
Species: Homo sapiens
Genetic Insert: Sox10
Vector Backbone Description: Backbone Marker:Kong-Jie Kah; Backbone Size:0; Vector Backbone:FUW-LPT2 (tetracycline-inducible); Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22385965
Comments: Human Sox10 cDNA, obtained from Harvard DNA Resource Core, was cloned between EcoRI and BamHI of FUW-LPT2.
Proper citation: RRID:Addgene_36978 Copy
Species: Homo sapiens
Genetic Insert: NRF2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Proper citation: RRID:Addgene_36971 Copy
Vector Backbone Description: Backbone Marker:Addgene plasmid #36953; Backbone Size:7052; Vector Backbone:pCMMP-MCS-IRES-eGFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318153
Comments: Sugden lab plasmid p3313
Addgene's sequencing results differ from the full plasmid sequence provided by the depositing by a single nucleotide insertion between bp# 2412&2413 and a four nucleotide deletion of bp #2493-2496. These differences are in IRES and the depositing laboratory verified the IRES is functional.
Proper citation: RRID:Addgene_36972 Copy
Species: Homo sapiens
Genetic Insert: NGFR:LMP1
Vector Backbone Description: Backbone Marker:Addgene plasmid #36972; Vector Backbone:pCMMP-MCS-IRES-mRFP; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22318153
Comments: Sugden lab plasmid p3856
The NGFR-LMP1 fusion in the plasmid contains the amino terminus of NGFR fused to the carboxy terminus of LMP1. The carboxy-terminal signaling of LMP1 is conditionally activated when anti-NGFR antibody crosslinks with the NGFR
moieties. Please see associated publication (link below) for more details.
Proper citation: RRID:Addgene_36974 Copy
Species: Homo sapiens
Genetic Insert: MOB1B
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:5472; Vector Backbone:pCl-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18362890
Proper citation: RRID:Addgene_37024 Copy
Species: Homo sapiens
Genetic Insert: CUL3
Vector Backbone Description: Backbone Marker:Moon Lab Plasmid available from Addgene (plasmid #15100); Backbone Size:5500; Vector Backbone:pGLUE; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22215675
Comments: Learn more about the pGLUE tandem-affinity purification plasmid backbone at http://www.addgene.org/15100/ .
Proper citation: RRID:Addgene_36970 Copy
Species: Homo sapiens
Genetic Insert: RASSF6b
Vector Backbone Description: Backbone Marker:Promega; Vector Backbone:pCl-neo (modified); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19797269
Proper citation: RRID:Addgene_37021 Copy
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