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Species: Synthetic
Genetic Insert: Pro-Pro-Pro-Pro-Pro
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2210; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27781345
Proper citation: RRID:Addgene_85098 Copy
Species: Bacillus megaterium
Genetic Insert: FADdomain of P450 BM3
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2227; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27781345
Comments: P450 BM3 was originally cloned from Bacillus megaterium (NBRC 15308) in our lab
Proper citation: RRID:Addgene_85104 Copy
Species: Synthetic
Genetic Insert: Putidaredoxin
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2210; Vector Backbone:pHSG398; Vector Types:Cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27781345
Comments: A synthetic gene of PdX was inserted between EcoRI and BamHI sites of pHSG398 using In-Fusion cloning kit.
Proper citation: RRID:Addgene_85100 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pBbS5c; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25496359
Comments: This plasmid carries G179V in Maqu_3494 compared to the genomic reference sequence. This mutation does not affect plasmid function.
Proper citation: RRID:Addgene_86205 Copy
Genetic Insert: Efflux pump
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pBbS5c; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25496359
Proper citation: RRID:Addgene_86203 Copy
Species: Synthetic
Genetic Insert: Pveg-sLucopt
Vector Backbone Description: Backbone Marker:Robert Fagan and Neil Fairweather; Backbone Size:6378; Vector Backbone:pRPF185; Vector Types:Bacterial Expression, Luciferase, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Please note that the individual components of this construct (that is, Pveg and sLucopt) are described in Oliveira Paiva AM et al (2016) ACS Synth Biol (DOI: 10.1021/acssynbio.6b00104). During the cloning a mutation occurred that removes the SacI site in between the Pveg and sLucopt genes. The 5' (KpnI) and 3' (BamHI) restriction sites remain.
Proper citation: RRID:Addgene_86554 Copy
Vector Backbone Description: Backbone Size:5328; Vector Backbone:pBBR; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28931397
Proper citation: RRID:Addgene_86709 Copy
Genetic Insert: gltA1-gltA2-udhA-GapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87151 Copy
Genetic Insert: gltA1-gltA2-gapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87150 Copy
Genetic Insert: fabI-gltA1-gltA2-udhA-zwf-gapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87149 Copy
Genetic Insert: gapAP1 guide array
Vector Backbone Description: Backbone Marker:None - Constructed in House; Backbone Size:2500; Vector Backbone:pCASCADE; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_87146 Copy
Species: Bacillus subtilis
Genetic Insert: XynA signal peptide
Vector Backbone Description: Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28230977
Proper citation: RRID:Addgene_87372 Copy
Genetic Insert: SpyTag
Vector Backbone Description: Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28230977
Proper citation: RRID:Addgene_87375 Copy
Genetic Insert: mSpyCatcher
Vector Backbone Description: Backbone Size:2676; Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28230977
Proper citation: RRID:Addgene_87374 Copy
Species: Groundwater metagenome
Genetic Insert: CasY1
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28005056
Proper citation: RRID:Addgene_87687 Copy
Species: Deltaproteobacteria
Genetic Insert: CasX1
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28005056
Proper citation: RRID:Addgene_87685 Copy
Genetic Insert: pkaiBC::EYFP
Vector Backbone Description: Backbone Marker:O'Shea lab; Vector Backbone:EB2065; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:28423314
Proper citation: RRID:Addgene_87753 Copy
Species: Other
Genetic Insert: uirR
Vector Backbone Description: Vector Backbone:colE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27120220
Proper citation: RRID:Addgene_78554 Copy
Species: Synthetic
Genetic Insert: BBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78691 Copy
Species: Synthetic
Genetic Insert: BBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
Vector Backbone Description: Backbone Marker:Registry of Standardized Biological Parts; Backbone Size:2047; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:27111289
Proper citation: RRID:Addgene_78693 Copy
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