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Species: Enterobacteria phage lambda
Genetic Insert: cI
Vector Backbone Description: Vector Backbone:pFG036; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:32963323
Comments: This plasmid was made to repress the expression of the transposase (pL promoter) in pFG051.
This plasmid has to be in the bacterium to avoid transposition of pFG051's transposon.
It has to be in the donor strain for conjugation to avoid unwanted transposition in the donor.
A strain carrying this plasmid was used to build pFG051.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_137996 Copy
Genetic Insert: FLPo
Vector Backbone Description: Backbone Size:3800; Vector Backbone:na; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17225864
Comments: FLPo is a mouse codon-optimized FLP.
Proper citation: RRID:Addgene_13793 Copy
Species: Homo sapiens
Genetic Insert: DELTA DED FLIP LONG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5600; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15209560
Proper citation: RRID:Addgene_13790 Copy
Species: Mus musculus
Genetic Insert: mouse Lin28A, CSD and ZnFs (residues 36–186)
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26045559
Proper citation: RRID:Addgene_137998 Copy
Species: Homo sapiens
Genetic Insert: DELTA DED FLIP LONG
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5600; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15209560
Proper citation: RRID:Addgene_13791 Copy
Species: Homo sapiens
Genetic Insert: gRNA library targeting human coding genome
Vector Backbone Description: Backbone Marker:Kosuke Yusa; Backbone Size:8649; Vector Backbone:Addgene Plasmid 67974: pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_137999 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137884 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-D2-B/c
Vector Backbone Description: Backbone Marker:Thermo Fisher Scientific; Backbone Size:10141; Vector Backbone:pMDC32; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:32396204
Proper citation: RRID:Addgene_137885 Copy
Vector Backbone Description: Vector Backbone:pZHY988; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32016614
Proper citation: RRID:Addgene_137888 Copy
Species: Synthetic
Genetic Insert: Superfolder GFP
Vector Backbone Description: Backbone Marker:Native to E. coli Nissle; Backbone Size:3173; Vector Backbone:pMUT1; Vector Types:Bacterial Expression, Synthetic Biology, For use with E. coli Nissle in the mouse gut; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33301298
Proper citation: RRID:Addgene_137921 Copy
Vector Backbone Description: Vector Backbone:pZHY988; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32016614
Proper citation: RRID:Addgene_137887 Copy
Species: Synthetic
Genetic Insert: mRFP1
Vector Backbone Description: Backbone Marker:Native to E. coli Nissle; Backbone Size:3173; Vector Backbone:pMUT2; Vector Types:Bacterial Expression, Synthetic Biology, For use with E. coli Nissle in the mouse gut; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33301298
Proper citation: RRID:Addgene_137923 Copy
Vector Backbone Description: Vector Backbone:pZHY988; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32016614
Proper citation: RRID:Addgene_137889 Copy
Species: Homo sapiens
Genetic Insert: SA-H2B-EGFP-bpA with homology arms
Vector Backbone Description: Backbone Size:2932; Vector Backbone:pBluescriptII SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433962
Proper citation: RRID:Addgene_137925 Copy
Species: Homo sapiens
Genetic Insert: SA-EGFP-bpA with homology arms
Vector Backbone Description: Backbone Size:2865; Vector Backbone:pBluescriptII SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433962
Proper citation: RRID:Addgene_137927 Copy
Species: Mus musculus
Genetic Insert: SA-EGFP-bpA with micro-homology arms
Vector Backbone Description: Backbone Size:2955; Vector Backbone:pBluescriptII SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433962
Proper citation: RRID:Addgene_137926 Copy
Species: Homo sapiens
Genetic Insert: SA-H2B-mCherry-bpA with homology arms
Vector Backbone Description: Backbone Size:2929; Vector Backbone:pBluescriptII SK+; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32433962
Proper citation: RRID:Addgene_137928 Copy
Species: Synthetic
Genetic Insert: J23101-3TG- pSEVA-331Bb- RFP
Vector Backbone Description: Vector Backbone:pSEVA-331Bb; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30865830
Comments: High expression
Proper citation: RRID:Addgene_138065 Copy
Species: Homo sapiens
Genetic Insert: SIRT1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1 +; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12620231
Comments: cloned into a version of pcDNA3.1 that has been modified to include a C-terminal Flag tag (see author's map for details).
Proper citation: RRID:Addgene_13812 Copy
Vector Backbone Description: Vector Backbone:pYPQ133A; Vector Types:Plant Expression, CRISPR; Bacterial Resistance:Tetracycline
Defining Citation: PMID:33782402
Proper citation: RRID:Addgene_138100 Copy
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