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Species: Mus musculus
Genetic Insert: Sema3e
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72020 Copy
Species: Mus musculus
Genetic Insert: Sema3f
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72023 Copy
Species: Mus musculus
Genetic Insert: Sema3g.z
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Sema3g.z contains a 105 nt deletion at the 3' end of exon 10.
Proper citation: RRID:Addgene_72027 Copy
Species: Mus musculus
Genetic Insert: Sema3g.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: We identified a previously-unreported isoform and named it "z". As such, we designated this previously-reported isoform as isoform "a".
Proper citation: RRID:Addgene_72025 Copy
Species: Mus musculus
Genetic Insert: Sema4b
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72029 Copy
Species: Mus musculus
Genetic Insert: Unc5b.z
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Unc5b.z is missing exon 8.
Proper citation: RRID:Addgene_72053 Copy
Species: Mus musculus
Genetic Insert: Unc5d.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: We identified two previously-unreported isoforms and named them "y" and "z". As such, we designated this previously-reported isoform as isoform "a".
Proper citation: RRID:Addgene_72057 Copy
Species: Mus musculus
Genetic Insert: Unc5c.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72054 Copy
Species: Mus musculus
Genetic Insert: Sema6c
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72041 Copy
Species: Mus musculus
Genetic Insert: Sema6d.1
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72042 Copy
Species: Mus musculus
Genetic Insert: Unc5c
Vector Backbone Description: Backbone Marker:Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72196 Copy
Species: Mus musculus
Genetic Insert: Unc5a
Vector Backbone Description: Backbone Marker:Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72194 Copy
Species: Mus musculus
Genetic Insert: Unc5d
Vector Backbone Description: Backbone Marker:Yves Durocher, National Research Council of Canada-Biotechnology Research Institute; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72197 Copy
Species: Mus musculus
Genetic Insert: Ntng1.g
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Addgene Sanger sequencing found F166L within the Lmnt1 translation compared to AF475075
Proper citation: RRID:Addgene_72116 Copy
Species: Mus musculus
Genetic Insert: Ntng1.d
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72113 Copy
Species: Mus musculus
Genetic Insert: Ntng1.e
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72114 Copy
Species: Mus musculus
Genetic Insert: Unc5c.y
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: Unc5c.y has a 57 nt insertion between exons 7 and 8.
Proper citation: RRID:Addgene_72181 Copy
Species: Mus musculus
Genetic Insert: Unc5d.a
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Comments: We identified two previously-unreported isoforms and named them "y" and "z". As such, we designated this previously-reported isoform as isoform "a".
Proper citation: RRID:Addgene_72183 Copy
Species: Mus musculus
Genetic Insert: Ten-Eleven Translocation 3
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21778364
Comments: Modified pcDNA3 vector with a beta globulin intron inserted. This insertion can help stabilize the transcripts and is particularly useful for the expression of large genes, such as p300 and Tet proteins
Note that an additional mutation, D1507E, is present in the Tet3 insert in this plasmid. This additional mutation does not affect the property of the mutant protein (as it is catalytically dead).
For additional information about how this plasmid was created and used, please see Ito et al., Nature 2010 Pubmed ID: 20639862
Proper citation: RRID:Addgene_72222 Copy
Species: Mus musculus
Genetic Insert: Rgma
Vector Backbone Description: Vector Backbone:pCMVi-SV40ori; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26633812
Proper citation: RRID:Addgene_72133 Copy
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