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Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:6005; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Comments: There are a few mismatches between author sequence and Addgene quality control sequence but they are outside of the LEU2 coding sequence and do not alter function of this plasmid.
Proper citation: RRID:Addgene_26849 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5150; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Comments: There are a few mismatches between author sequence and Addgene quality control sequence but they are outside of the LEU2 coding sequence and do not alter function of this plasmid.
Proper citation: RRID:Addgene_26847 Copy
Vector Backbone Description: Backbone Size:4822; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26846 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:6488; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26841 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:7587; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26840 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaspeR4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Proper citation: RRID:Addgene_26859 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26856 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. To express Myc-tagged wild-type STOPS in flies, the sequence encoding a Myc tag was fused to the 3' end
of the wild-type stops cDNA. The wild-type stops cDNAs was
subsequently subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26855 Copy
Species: Drosophila melanogaster
Genetic Insert: Slow Termination of Phototransduction
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express the stops cDNA under the control of the ninaE promoter, the cDNA
(EST clone GH07253, DGRC) was subcloned between the NotI and XbaI sites
of pCNX. Myc-tagged STOPS lacking the SOCS box was
subcloned between the NotI and XbaI sites of pCNX.
The pCNX vector was constructed as follows. First, the pNX vector (provided by Dr. J. O'Tousa, University of Notre Dame, Notre Dame, IN) was digested with KpnI and NotI, which liberated a 3 kb ninaE promoter sequence, and this fragment was subsequently introduced between the same sites of pCaspeR4. Second, to provide the signals for polyadenylation and 3' end cleavage of RNAs, a simian virus 40 3' region (0.5 kb) was excised from pCaspeR–heat shock protein 70 promoter (hs) and introduced between the XbaI and XhoI sites of the vector
Proper citation: RRID:Addgene_26857 Copy
Genetic Insert: LoxP-3xPDsRed-LoxP
Vector Backbone Description: Backbone Size:4556; Vector Backbone:pXL-BACII; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: pXL-BACII transformation vector marked by a floxed eye expressing DsRed expression cassette. Cloning sites are EcoRV, SalI, XhoI.
Proper citation: RRID:Addgene_26852 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Gal80
Vector Backbone Description: Backbone Size:11270; Vector Backbone:pGalW; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: P-element based GAL80 enhancer trap
Proper citation: RRID:Addgene_26851 Copy
Species: Drosophila melanogaster
Genetic Insert: no receptor potential A
Vector Backbone Description: Backbone Size:0; Vector Backbone:pCNX; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18184564
Comments: To express norpA under control of the ninaE promoter, full length norpA cDNA was excised from pHKX-norpA and subcloned into pCNX
Proper citation: RRID:Addgene_26854 Copy
Species: recoded CreA for expression in S. cerevisiae
Genetic Insert: CreA recombinase
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:7226; Vector Backbone:pYES2.0; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26853 Copy
Species: recoded CreA recombinase for expression in S. cerevisiae
Genetic Insert: CreA recombinase
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:7209; Vector Backbone:pYES2.0; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26850 Copy
Vector Backbone Description: Backbone Size:5431; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26826 Copy
Vector Backbone Description: Backbone Size:6738; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26829 Copy
Species: jellyfish
Genetic Insert: 5'UTR-eGFP-3'UTR
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pcDNA3.3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20888316
Comments: Note that orientation of the insert in pcDNA does not matter -- they were cloned by TA-cloning, and either orientation is fine.
Information regarding the UTR sequences and details regarding cloning can be found in the associated publication (http://www.cell.com/cms/attachment/609702/4870828/mmc1.pdf)
Proper citation: RRID:Addgene_26822 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5371; Vector Backbone:pUC18; Vector Types:Yeast Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26825 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5181; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26838 Copy
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5446; Vector Backbone:pUC18; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20936606
Proper citation: RRID:Addgene_26839 Copy
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