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Species: Homo sapiens
Genetic Insert: RAB11B, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for integration of SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out) to Rab11b locus
This can be combined with knock in of DExCon module to be able to reversibly rescue Rab11b expression (from the second allele) on demand
DExCon = Doxycycline-mediated endogenous gene Expression Control
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179893 Copy
Species: Homo sapiens
Genetic Insert: RAB11A, member RAS oncogene family
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:3000; Vector Backbone:pJet1.2; Vector Types:Mammalian Expression, Bacterial Expression, CRISPR, Donor for homologous based knock in; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35708998
Comments: Here donor for mNeonGreen knock in to Rab11a.
https://figshare.com/projects/DExCon_DExogron_LUXon_on-demand_expression_control_of_endogenous_genes_reveals_differential_dynamics_of_Rab11_family_members/118281.
Please visit https://www.biorxiv.org/content/10.1101/2021.12.03.471086v1.full for bioRxiv preprint.
Proper citation: RRID:Addgene_179890 Copy
Species: E. coli
Genetic Insert: Scrambled sgRNA targeting sequences
Vector Backbone Description: Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35636511
Proper citation: RRID:Addgene_177812 Copy
Species: Synthetic
Genetic Insert: HyperdCas12a
Vector Backbone Description: Vector Backbone:PB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35414015
Proper citation: RRID:Addgene_183964 Copy
Species: Homo sapiens
Genetic Insert: SHP2
Vector Backbone Description: Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_8381 Copy
Species: Lachnospiraceae bacterium
Genetic Insert: LbCas12a
Vector Backbone Description: Vector Backbone:pSC101 ori; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:35659325
Comments: The sequence of LbCas12a was from Addgene plasmid # 113431 ; http://n2t.net/addgene:113431 ; RRID:Addgene_113431
Proper citation: RRID:Addgene_183074 Copy
Species: Caenorhabditis elegans
Genetic Insert: MCS mosTI unc-119 target
Vector Backbone Description: Vector Backbone:pTwist+Amp+High+Copy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35900171
Comments: See www.wormbuilder.org for more information. Please visit https://www.biorxiv.org/content/10.1101/2022.04.19.488726v3 for bioRxiv preprint.
Proper citation: RRID:Addgene_159821 Copy
Species: Synthetic
Genetic Insert: huAsCpf1
Vector Backbone Description: Backbone Size:8069; Vector Backbone:pHKO_23; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27918548
Comments: Contains a BsmBI cloning cassette for insertion of Cpf1 guides under U6 promoter control.
Proper citation: RRID:Addgene_84739 Copy
Species: Caenorhabditis elegans
Genetic Insert: MRP-1
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:4702; Vector Backbone:pPD95.77_SL2; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35513700
Proper citation: RRID:Addgene_184129 Copy
Species: Caenorhabditis elegans
Genetic Insert: CELE_VW02B12L.1
Vector Backbone Description: Backbone Marker:Piali Sengupta Lab; Backbone Size:4194; Vector Backbone:coel::rfp; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35513700
Proper citation: RRID:Addgene_184134 Copy
Species: Caenorhabditis elegans
Genetic Insert: MRP-1
Vector Backbone Description: Backbone Marker:Fire Lab; Backbone Size:4376; Vector Backbone:pPD95.75; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35513700
Proper citation: RRID:Addgene_184131 Copy
Species: Other
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35149555
Proper citation: RRID:Addgene_184637 Copy
Species: Homo sapiens
Genetic Insert: RAF1
Vector Backbone Description: Vector Backbone:Tet-pLKO-puro; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_185371 Copy
Species: Arabidopsis thaliana
Genetic Insert: sgRNA cassette under U3 promoter
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2974; Vector Backbone:pJet1.2; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35022409
Comments: This vector was designed and constructed by Dr. Tomasz Bieluszewski.
Detailed protocol for CRISPR/Cas9-based mutagenesis using this system in:
Bieluszewski T, Szymanska-Lejman M, Dziegielewski W, Zhu L, Ziolkowski PA. Efficient Generation of CRISPR/Cas9-Based Mutants Supported by Fluorescent Seed Selection in Different Arabidopsis Accessions, in: Plant Gametogenesis: Methods and Protocols (ed. Christophe Lambing), Methods in Molecular Biology, vol. 2484.
https://doi.org/10.1007/978-1-0716-2253-7_13
https://pubmed.ncbi.nlm.nih.gov/35461452/
Proper citation: RRID:Addgene_173156 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-OMICRON.BA.2
Vector Backbone Description: Backbone Size:4745; Vector Backbone:pαH; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35732171
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_184829 Copy
Species: Severe acute respiratory syndrome coronavirus 2
Genetic Insert: S-GSAS-OMICRON.BA2-RBD
Vector Backbone Description: Backbone Size:4681; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35732171
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487528v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_184830 Copy
Genetic Insert: SARS-CoV-2 Spike BA.1
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35216664
Proper citation: RRID:Addgene_185452 Copy
Genetic Insert: SARS-CoV-2 D614G Spike
Vector Backbone Description: Backbone Size:4717; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36254158
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.02.19.481107v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_185692 Copy
Genetic Insert: SARS-CoV-2 BA.4/5 Spike
Vector Backbone Description: Backbone Size:4716; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36656710
Comments: Please visit https://doi.org/10.1101/2022.08.03.22278386 for medRxiv preprint.
Proper citation: RRID:Addgene_186031 Copy
Vector Backbone Description: Vector Backbone:pBRN169-MXMT; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29661017
Proper citation: RRID:Addgene_182790 Copy
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