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Species: Homo sapiens
Genetic Insert: Engineered target sites miR-148a-3p
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4637; Vector Backbone:pGL4.13; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24895996
Proper citation: RRID:Addgene_65053 Copy
Species: Homo sapiens
Genetic Insert: hsa-miR-216a (pre-miRNA)
Vector Backbone Description: Backbone Size:5175; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24895996
Proper citation: RRID:Addgene_65054 Copy
Species: Rattus norvegicus
Genetic Insert: DDIT4
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pCMS-EGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17005863
Comments: There are 2 mutations if compared to PubMed DDIT4 R. Norvegicus sequence (L121V and S208G) because the insert was cloned out of DC12 cells DNA.
Proper citation: RRID:Addgene_65057 Copy
Species: Homo sapiens
Genetic Insert: Delta(Pro-MP)ADAM10
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12743035
Comments: Mutagenesis creates EcoRI site 57bp downstream of Start ATG
Proper citation: RRID:Addgene_65107 Copy
Species: Synthetic
Genetic Insert: MS2
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pMal-c; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18570876
Comments: The following reference describes how to isolate RNA binding complexes using this construct:
Jurica and Moore, Methods. 2002 Nov;28(3):336-45. https://www.ncbi.nlm.nih.gov/pubmed/12431437
Proper citation: RRID:Addgene_65104 Copy
Genetic Insert: ABKAR biosensor
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25892241
Proper citation: RRID:Addgene_65068 Copy
Genetic Insert: RBD, mCerulean, mVenus, RhoC
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5200; Vector Backbone:pTriEx; Vector Types:Mammalian Expression, Bacterial Expression, Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24224016
Proper citation: RRID:Addgene_65071 Copy
Vector Backbone Description: Backbone Size:9326; Vector Backbone:pdCas9-Marraffini (pACYC184); Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25822415
Proper citation: RRID:Addgene_65006 Copy
Species: Mus musculus
Genetic Insert: Ii-Streptavidin and truncated Mannosidase II
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65257 Copy
Species: Mus musculus
Genetic Insert: Streptavidin-KDEL and truncated Mannosidase II
Vector Backbone Description: Backbone Marker:system biosciences; Vector Backbone:pCDH-CMV-MCS-EF1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65259 Copy
Species: Mus musculus
Genetic Insert: Streptavidin-KDEL and truncated Mannosidase II
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRES_neo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65253 Copy
Species: Mus musculus
Genetic Insert: Ii-Streptavidin and truncated Mannosidase II
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65255 Copy
Species: Synthetic
Genetic Insert: GFP dropout
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 234r is used for the construction of dropout vectors. This plasmid encodes for a fluorescent dropout. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65154 Copy
Species: Aequorea victoria
Genetic Insert: EGFP
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3001; Vector Backbone:pGEM5Z(+); Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19248754
Comments: To be used in conjunction with pGEM5Z(+)-EGFP (p111): www.addgene.org/65206. See corrigendum/erratum for original publication (https://doi.org/10.1016/j.ab.2009.10.024) before performing experiments with this plasmid.
Proper citation: RRID:Addgene_65207 Copy
Species: Synthetic
Genetic Insert: ConR1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 5 parts are 3' assembly connectors in the Duebery YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65174 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6822.
VP1 capsid ORF utilizes a non-canonical initiation codon (TTG codon) beginning at nt 6514.
Additional article references: Barbash et al. Gene Therapy 20: 274-282 (2013).
Proper citation: RRID:Addgene_65215 Copy
Species: Other
Genetic Insert: modified AAV-2 rep78
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5238; Vector Backbone:pFastBac-Dual; Vector Types:Insect Expression, AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19532142
Comments: Rep78 ORF utilizes a non-canonical initiation codon (CTG codon) beginning at nt 6824.
VP1 capsid ORF utilizes a non-canonical initiation codon (ACG codon) beginning at nt 6511.
Proper citation: RRID:Addgene_65214 Copy
Species: Homo sapiens
Genetic Insert: esr1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAcGFP1-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_65211 Copy
Species: Synthetic
Genetic Insert: NourseothricinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65185 Copy
Species: Synthetic
Genetic Insert: KanamycinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65184 Copy
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