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Species: Rattus norvegicus
Genetic Insert: shCrh(2)
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11657; Backbone Size:8491; Vector Backbone:pPRIME-CMV-GFP-recipient; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31577943
Proper citation: RRID:Addgene_132710 Copy
Species: Rattus norvegicus
Genetic Insert: shCrh(1)
Vector Backbone Description: Backbone Marker:Addgene Plasmid #11657; Backbone Size:8491; Vector Backbone:pPRIME-CMV-GFP-recipient; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31577943
Proper citation: RRID:Addgene_132709 Copy
Species: RSF1010 plasmid
Genetic Insert: MobA
Vector Backbone Description: Backbone Marker:-; Backbone Size:17500; Vector Backbone:pRL623; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31585408
Comments: Please note that Addgene found mutations Q291P, P416T, P595S, H623D in mobA during our quality control process. The depositing lab confirmed these mutations do not affect plasmid function, and the plasmid functions as described in the associated publication.
Proper citation: RRID:Addgene_132664 Copy
Species: Synthetic
Genetic Insert: sfGFP
Vector Backbone Description: Backbone Size:2970; Vector Backbone:pFA; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31406332
Proper citation: RRID:Addgene_133009 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9 NLS
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31719930
Proper citation: RRID:Addgene_133604 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9 NLS Stop
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31719930
Proper citation: RRID:Addgene_133602 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sgTEF1
Vector Backbone Description: Vector Backbone:pYTK050; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31719930
Proper citation: RRID:Addgene_133601 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mxi1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31719930
Proper citation: RRID:Addgene_133597 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Mxi1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31719930
Proper citation: RRID:Addgene_133596 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31719930
Proper citation: RRID:Addgene_133593 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31719930
Proper citation: RRID:Addgene_133592 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Linus
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31719930
Proper citation: RRID:Addgene_133590 Copy
Species: Other
Genetic Insert: sfGFP(SPS6)
Vector Backbone Description: Backbone Marker:Frenzel etal. 2018; Vector Backbone:pNW-Ppta-3TER; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29195004
Proper citation: RRID:Addgene_133814 Copy
Species: Synthetic
Genetic Insert: sfGFP(Sp)
Vector Backbone Description: Vector Backbone:pAD43-25; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29195004
Proper citation: RRID:Addgene_133833 Copy
Species: Synthetic
Genetic Insert: mKate(KPS12)
Vector Backbone Description: Backbone Marker:Dunn and Handelsman, 1999; Vector Backbone:pAD43-25; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29195004
Proper citation: RRID:Addgene_133831 Copy
Species: Synthetic
Genetic Insert: sfGFP(SPS6)
Vector Backbone Description: Backbone Marker:Dunn and Handelsman, 1999; Vector Backbone:pAD43-25; Vector Types:Bacterial Expression, E.coli/Bacillus spp. shuttle vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29195004
Proper citation: RRID:Addgene_133906 Copy
Vector Backbone Description: Backbone Size:11151; Vector Backbone:pBAC-lacZ; Vector Types:Bacterial Expression, Cre/Lox; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17406455
Comments: The complete assembled sequence for this plasmid may not be accurate. Addgene suggests sequence verifying the construct before starting your experiments.
Bacterial cell-based two-hybrid (B2H) reporter vector. Use 12.5 ug/ml chloramphenicol for growth.
The pBAC-lacZ plasmid is a mini-F' that can be replicated in standard E. coli strains, but because it is maintained as a single copy episome, it gives low DNA yields. pBAC-lacZ also contains a second, higher copy number origin of replication (oriV) that is only active in the presence of a trans-acting factor encoded by the trfA gene. Transformax EPI300 cells express trfA from an
inducible promoter that we have found is inducible with arabinose. When the trfA gene is induced in Transformax EPI300 cells, the copy number of pBAC-lacZ plasmid is increased in the cells and reasonable yields of plasmid can be obtained using a standard miniprep procedure.
Further notes on propagating this plasmid: grow an overnight culture (without arabinose) and then the next morning subculture 1:10 into medium that has a final concentration of arabinose of 1 mM. You then grow this culture for 5 to 6 hours at 37 C and then harvest the DNA.
Proper citation: RRID:Addgene_13422 Copy
Species: Synthetic
Genetic Insert: cmR_gp41-1 N-intein + cmR_gp41-1 C-intein
Vector Backbone Description: Vector Backbone:pBAD33 + pTrc99a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31672972
Comments: pSiMPlc_N should be cotransformed with pSiMPlc_C (or vice versa) in E. Coli to get a reconstituted functional CAT thereby conferring resistance against chloramphenicol. mRuby3 gene was amplified from a construct bought by us from Addgene (ID: 74252).
Please see the Sequences link for the individual plasmid sequences.
The sample from Addgene will have both the plasmids pSiMPlc_N + pSiMPlc_C in it for the interested
antibiotic resistance. Separating individual plasmid backbones will be possible via PCR. Agarose gel electrophoresis can be used to visualize the presence of both the plasmids. But, separating individual plasmids via gel electrophoresis, in our hands, always resulted in cross-contamination of the two backbones.
Proper citation: RRID:Addgene_134312 Copy
Species: Synthetic
Genetic Insert: rbs-msfGFPC-H3H4
Vector Backbone Description: Vector Backbone:pHCL149; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31550057
Proper citation: RRID:Addgene_134456 Copy
Species: Synthetic
Genetic Insert: Para-popZ-rbs-H3H4-msfGFPN-CTM.
Vector Backbone Description: Vector Backbone:pTB285; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31550057
Proper citation: RRID:Addgene_134457 Copy
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