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Species: Synthetic
Genetic Insert: dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin) with His6-maltose binding protein fusion tag
Vector Backbone Description: Vector Backbone:pDonr235; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162950 Copy
Species: Synthetic
Genetic Insert: His6 purification tag with upstream HTLV RU5 5' UTR
Vector Backbone Description: Vector Backbone:pDonr235; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162953 Copy
Species: Synthetic
Genetic Insert: His6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR
Vector Backbone Description: Vector Backbone:pDonr235; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162955 Copy
Species: Synthetic
Genetic Insert: His6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
Vector Backbone Description: Vector Backbone:pDonr235; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162957 Copy
Species: Autographa californica multiple nucleopolyhedrovirus
Genetic Insert: AcMNPV polyhedrin promoter with upstream polyA sequence
Vector Backbone Description: Vector Backbone:pDonr235; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162948 Copy
Species: Synthetic
Genetic Insert: monomeric Teal fluorescent protein
Vector Backbone Description: Vector Backbone:pDonr235; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162942 Copy
Species: Synthetic
Genetic Insert: monomeric Ruby3 fluorescent protein
Vector Backbone Description: Vector Backbone:pDonr235; Vector Types:Synthetic Biology; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_162945 Copy
Species: Mus musculus
Genetic Insert: RpH-LAMP1-3xFLAG
Vector Backbone Description: Vector Backbone:pDONOR223; Vector Types:GATEWAY system; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:32515674
Proper citation: RRID:Addgene_163019 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15790532
Proper citation: RRID:Addgene_29357 Copy
Species: Homo sapiens
Genetic Insert: DJ1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:15790532
Proper citation: RRID:Addgene_29354 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: sgf11
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:3620; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:20395473
Proper citation: RRID:Addgene_26960 Copy
Species: E. coli
Genetic Insert: dnaK, dnaJ
Vector Backbone Description: Backbone Size:7072; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Please note that Addgene's sequencing results detect a single nucleotide insertion compared to depositor's sequence in the vector backbone. The plasmid expresses the expected functional protein.
Proper citation: RRID:Addgene_27392 Copy
Species: E. coli
Genetic Insert: groESL
Vector Backbone Description: Backbone Size:0; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27394 Copy
Species: Homo sapiens
Genetic Insert: ABL2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23909 Copy
Species: Homo sapiens
Genetic Insert: MERTK
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23900 Copy
Species: Homo sapiens
Genetic Insert: PTK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23902 Copy
Species: Homo sapiens
Genetic Insert: STYK1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23903 Copy
Species: Homo sapiens
Genetic Insert: UCK2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23863 Copy
Species: Homo sapiens
Genetic Insert: MAPK14
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23865 Copy
Species: Homo sapiens
Genetic Insert: RPS6KA1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5005; Vector Backbone:pDONR223; Vector Types:Gateway Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:21107320
Comments: The ORF has no Stop codon. This design enables easy recombination into a destination vector to add an epitope tag of choice and Stop codon to the ORF. Do NOT use this plasmid directly for transfections.
A set of 4 custom lentiviral vectors is available ( pLX301 (http://www.addgene.org/25895/) , pLX302 (http://www.addgene.org/25896/) , pLX303 (http://www.addgene.org/25897/) , pLX304 (http://www.addgene.org/25890/) ) to be used to express these ORFs in mammalian cells. These plasmids are not included in the kit and must be ordered separately.
Proper citation: RRID:Addgene_23860 Copy
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