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Species: Synthetic
Genetic Insert: 4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16720273
Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and circularly permuted Venus 173. D3cpv does not bind wild-type calmodulin. It has a Kd' of 0.6 uM.
See also Palmer & Tsien (2006) Nature Protocols.
The small discrepancy between the Addgene and depositor's sequence should not alter the function of the plasmid.
Proper citation: RRID:Addgene_36324 Copy
Species: Synthetic
Genetic Insert: Golgi-ZapCY1 high affinity Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21502528
Comments: Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
Targeted to Golgi: N-term fusion of the first 60 amino acids of GalT
Proper citation: RRID:Addgene_36322 Copy
Vector Backbone Description: Backbone Marker:Vieira and Messing, 1991; Backbone Size:3868; Vector Backbone:pUK21; Vector Types:Bacterial Expression, Suicide vector for R. rubrum; Bacterial Resistance:Kanamycin
Comments: Constructed by D. P. Lies and G. P. Roberts
Proper citation: RRID:Addgene_36328 Copy
Species: Synthetic
Genetic Insert: ER-ZapCY1 high affinity Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21502528
Comments: Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
Targeted to ER: contains C-terminal KDEL
Proper citation: RRID:Addgene_36321 Copy
Species: Synthetic
Genetic Insert: ZapCY2 high affinity Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21502528
Comments: Second generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from the first 2 zinc fingers of Zap 1 from Saccharomyces cerevisiae. Contains truncated CFP and Citrine fluorescent proteins.
ZapCY2 is derived from ZapCY1. The first cysteine in each zinc finger is mutated to histidine.
Proper citation: RRID:Addgene_36320 Copy
Genetic Insert: Dimerization dependent RFP-B1
Vector Backbone Description: Backbone Marker:Life Technologies; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22444590
Proper citation: RRID:Addgene_36290 Copy
Species: Rattus norvegicus
Genetic Insert: G protein alpha 0
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:7261; Vector Backbone:pd12.2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15078878
Comments: The CFP was introduced between 92nd and 93rd amino acid of alpha-0
Proper citation: RRID:Addgene_36312 Copy
Genetic Insert: shRNA of scramble
Vector Backbone Description: Backbone Marker:Biosettia; Backbone Size:10275; Vector Backbone:pLV-H1TetO-RFP-Puro; Vector Types:Mammalian Expression, Lentiviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22645348
Comments: The plasmid backbone is TetO-H1-shRNA-EF1a-TetR-IRES-RFP-Puro. It is a all-in-one inducible shRNA knockdown lentiviral plasmid.
The paper associated with this plasmid is "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling"
Proper citation: RRID:Addgene_36311 Copy
Species: Synthetic
Genetic Insert: mito-ZifCY1 genetically encoded Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19363034
Comments: First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. mito-ZifCY1 contains 4 tandem repeats of the first 29 aa of human cox subunit 8a (MSVLTPLLLRGLTGSARRLPVPRAKIHSL). HindIII restriction sites are at the 5' and 3' ends of the mitochondrial targeting sequence.
Proper citation: RRID:Addgene_36317 Copy
Species: Synthetic
Genetic Insert: ZifCY2 genetically encoded Zn(II) sensor
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5500; Vector Backbone:pcDNA3.1(+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19363034
Comments: First generation genetically encoded, ratiometric, fluorescent biosensor. Zn(II)-binding domain derived from mammalian Zif268. ZifCY2 is a variant of ZifCY1. The two cysteines (amino acids 237 and 242) in the Zn(II)-binding domain of ZifCY1 are mutated to histidines in ZifCY2.
Proper citation: RRID:Addgene_36316 Copy
Genetic Insert: pXylan
Vector Backbone Description: Vector Backbone:Biobrick family of vectors; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22123987
Comments: Note from depositor regarding growing this plasmid: W used DH10B, plated on LB/carb, and grew the plasmid up in TB medium plus 2% glycerol and 100 ug/mL carbenicillin. It's key to not grow the cells up too long, either on the plate or in the liquid medium, and probably best to harvest the cells in early stationary phase.
Proper citation: RRID:Addgene_36301 Copy
Species: Homo sapiens
Genetic Insert: EZH2
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36389 Copy
Species: Homo sapiens
Genetic Insert: ubiquitin specific peptidase 19
Vector Backbone Description: Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21849505
Proper citation: RRID:Addgene_36307 Copy
Species: Homo sapiens
Genetic Insert: DNMT1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Comments: Addgene's quality control sequence varies slightly from the depositor's, but this plasmid should be functional.
Proper citation: RRID:Addgene_36383 Copy
Species: Homo sapiens
Genetic Insert: EED
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36386 Copy
Species: Homo sapiens
Genetic Insert: DNMT1
Vector Backbone Description: Backbone Marker:Steve Elledge; Backbone Size:6699; Vector Backbone:pSMP; Vector Types:Mammalian Expression, Retroviral, RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22388813
Proper citation: RRID:Addgene_36384 Copy
Species: Mus musculus
Genetic Insert: mTERT
Vector Backbone Description: Backbone Marker:Bob Weinberg (Addgene Plasmid #1764); Backbone Size:5169; Vector Backbone:pBABE-puro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21732358
Proper citation: RRID:Addgene_36413 Copy
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Plasmid carrying monomeric (A206K) EGFP for N-terminal tagging
Proper citation: RRID:Addgene_36412 Copy
Species: Homo sapiens
Genetic Insert: active BCR-related
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:17116687
Comments: This is the ABR isoform A. The R683A N795A double mutant lacks GTPase activity towards RacGTP. Construct: Young-jin Cho. Also see PMID 20962234.
Proper citation: RRID:Addgene_36417 Copy
Species: Homo sapiens
Genetic Insert: breakpoint cluster region protein isoform 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20962234
Comments: Also see PMID 17116687.
Proper citation: RRID:Addgene_36416 Copy
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