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Species: Streptomyces sp. CNR 698
Genetic Insert: ammA(delW56)
Vector Backbone Description: Vector Backbone:pACYCDuet1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:31320540
Proper citation: RRID:Addgene_134683 Copy
Species: Synthetic
Genetic Insert: PBAD-EGFP-151TGA
Vector Backbone Description: Vector Backbone:de novo assembled; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29449507
Comments: pUC
Proper citation: RRID:Addgene_107886 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Vector Backbone:p15aC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30944849
Proper citation: RRID:Addgene_107862 Copy
Species: Synthetic
Genetic Insert: T7RNAP (modified)
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29472537
Comments: Please visit https://www.biorxiv.org/content/early/2017/10/17/204925 for bioRxiv preprint.
Proper citation: RRID:Addgene_107957 Copy
Species: Synthetic
Genetic Insert: T7RNAP (modified)
Vector Backbone Description: Vector Backbone:pBAD33; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29472537
Comments: Please visit https://www.biorxiv.org/content/early/2017/10/17/204925 for bioRxiv preprint.
Proper citation: RRID:Addgene_107955 Copy
Species: Vibrio fischeri
Genetic Insert: luxI
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29472537
Comments: Please visit https://www.biorxiv.org/content/early/2017/10/17/204925 for bioRxiv preprint.
Proper citation: RRID:Addgene_107965 Copy
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30478458
Comments: The three new “Marionette” strains contain the Marionette cluster, which expresses 12 inducible transcription factors, from the genome. Plasmids pAJM.711, pAJM.712, pAJM.713, pAJM.714, pAJM.715, pAJM.717, pAJM.716, pAJM.718, pAJM.719, pAJM.1459, pAJM.721 are reporters for those 12 transcription factors. For examples: TetR is being expressed from the genome of the Marionette strains, and pAJM.717 has a promoter that is repressed by TetR (PTet*) driving YFP. pAJM.717 should not produce YFP, until the cell is exposed to anhydrotetracycline (aTc). Other plasmids from our laboratory encode both the reporter and the transcription factor, and should be used in a regular (non-Marionette) strain. For example: pAJM.011 expresses TetR and contains a PTet*-YFP reporter module.
Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
Proper citation: RRID:Addgene_108254 Copy
Species: Synthetic
Genetic Insert: Putidaredoxin
Vector Backbone Description: Backbone Marker:TaKaRa; Backbone Size:2230; Vector Backbone:pHSG398; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29663675
Proper citation: RRID:Addgene_108203 Copy
Species: Synthetic
Genetic Insert: Bxb1 attB site
Vector Backbone Description: Backbone Marker:Kenan Murphy; Vector Backbone:pKM435; Vector Types:ORBIT integration plasmid for creating knockouts of target genes; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT integrating plasmid for creating knockouts of target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108322 Copy
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBC SK+; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11771662
Proper citation: RRID:Addgene_108311 Copy
Species: Synthetic
Genetic Insert: attB-eGFP-4xGly-TEV-Flag-6xHis
Vector Backbone Description: Backbone Marker:Kenan Murphy; Backbone Size:3159; Vector Backbone:pKM446; Vector Types:ORBIT integrating plasmid; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:30538179
Comments: ORBIT plasmid for creating EGFP fusions to target genes.
Strain should be grown in 20 ug/ml chloramphenicol for isolation of plasmid. Yields with Qiagen miniprep kits are typically in the range from 60-90 ug/ml from 5 ml culture.
Proper citation: RRID:Addgene_108434 Copy
Species: Synthetic
Genetic Insert: dCas9-PmCDA1-UGI-LVA
Vector Backbone Description: Backbone Size:9263; Vector Backbone:pSC101; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29403014
Proper citation: RRID:Addgene_108551 Copy
Vector Backbone Description: Backbone Size:9550; Vector Backbone:p15A; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33095053
Proper citation: RRID:Addgene_104147 Copy
Species: Synthetic
Genetic Insert: Plpp MmAcK3RS(IPYE); PproK pyl
Vector Backbone Description: Backbone Marker:Liu Lab; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_104071 Copy
Species: Synthetic
Genetic Insert: Plpp MbPylRS(IPYE); PproK pyl
Vector Backbone Description: Backbone Marker:Liu Lab; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_104072 Copy
Species: Synthetic
Genetic Insert: Plpp chAcK3RS(IPYE); PproK pyl
Vector Backbone Description: Backbone Marker:Liu Lab; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29035361
Proper citation: RRID:Addgene_104069 Copy
Species: Escherichia coli str. K-12 substr. MG1655
Genetic Insert: OmpR
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29650958
Proper citation: RRID:Addgene_104335 Copy
Genetic Insert: PTet-cas12, tetR, lacI-treR
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29170279
Proper citation: RRID:Addgene_104574 Copy
Genetic Insert: PTet-cas12, tetR
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:29170279
Proper citation: RRID:Addgene_104575 Copy
Species: Myxococcus xanthus
Genetic Insert: MXAN_1745
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4008; Vector Backbone:pACYCDuet; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Proper citation: RRID:Addgene_104978 Copy
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