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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA
 
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RRID:Addgene_37082 hChr2(H134R)-mCherry Synthetic Ampicillin PMID:22660328 Backbone Marker:K. Deisseroth Lab; Backbone Size:5613; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-Off; Bacterial Resistance:Ampicillin 2026-08-15 01:14:12 7
pAAV-Ef1a-DIO-mCherry-WPRE-pA
 
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RRID:Addgene_37083 mCherry Synthetic Ampicillin PMID:22866029 Backbone Marker:K. Deisseroth Lab; Backbone Size:5603; Vector Backbone:pAAV-Ef1a-DIO-hChR2(H134R)-mCherry-WPRE-pA; Vector Types:AAV, Cre/Lox, Cre-On; Bacterial Resistance:Ampicillin 2026-08-15 01:14:12 6
pET15b-eIF4E
 
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RRID:Addgene_37228 eIF4E Saccharomyces cerevisiae Ampicillin PMID:20864040 Backbone Marker:EMD bioscience; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
pET15b-eIF4E-S200C
 
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RRID:Addgene_37229 eIF4E Saccharomyces cerevisiae Ampicillin PMID:20864040 Backbone Marker:EMD bioscience; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin S200C 2026-08-15 01:14:13 0
pUC19-HHtRNA
 
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RRID:Addgene_37222 HHtRNA Saccharomyces cerevisiae Ampicillin PMID:17913637 HHtRNA sequence (rev orientation): TGGTAGCGCCGCTCGGTTTCGATCCGAGGACATCAGGGTTATGAGCCCTGCGCGCTTCCACTGCGCCACGGCGCTGACGGTACCGGGTACCGTTTCGTCCTCACGGACTCATCAGAGCG Backbone Size:2686; Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
pET PPL His6 MBP LIC cloning vector (2K-T)
 
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RRID:Addgene_37183 Ampicillin This plasmid is an empty vector. Your gene can be inserted with a LIC cloning protocol. All 2-series vectors work as single-expression vectors, as well as transfer vectors for our polycistronic system. 2K-T has a TEV-cleavable N-terminal His6 and MBP fusion tag to enhance solubility and ease purification. These tags are preceded by a periplasmic locator signal, which is autocleaved during secretion. The PPL tag may be helpful if your protein binds to or interferes with intracellular E. coli molecules, since your protein of interest is safely sequestered in the periplasm. It may also be helpful if your protein of interest is more stable in the periplasmic environment. Protein purification may also be made simpler using this expression method. The LIC cloning site is flanked by 5 pairs of restriction sites, so that your gene can easily be subcloned into our polycistronic destination vectors (2D, 2E, or 2Z). To clone into this vector, add LIC v1 tags to the 5' end of your PCR primers. Forward - 5'TACTTCCAATCCAATGCA3' Reverse - 5'TTATCCACTTCCAATGTTATTA3' Linearize the plasmid with SspI and gel purify. When digesting the DNA with T4 polymerase, use dCTP for insert and dGTP for vector. More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/ Backbone Size:6025; Vector Backbone:pET; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 3
pCAG-T7-TALEN(Sangamo)-Destination
 
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RRID:Addgene_37184 Ampicillin PMID:24747757 For more information on Pelczar TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#pelczar Backbone Marker:CLONTECH; Backbone Size:4810; Vector Backbone:pDIRECT; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 3
pSG5 EDD C2768A
 
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RRID:Addgene_37192 EDD C2768A Homo sapiens Ampicillin PMID:12011095 The depositing lab has noted that mutation K503R shouldn’t be a problem as it is outside the main functional domains. There does appear to be a relatively common SNP at K503 in Latino populations so this might be a genuine SNP- http://exac.broadinstitute.org/variant/8-103338864-C-T https://www.ncbi.nlm.nih.gov/projects/SNP/snp_ref.cgi?rs=rs571861064 Backbone Marker:Stratagene; Backbone Size:4225; Vector Backbone:pSG5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Changed Cysteine 2768 to Alanine; K503R (please see depositor comments below) 2026-08-15 01:14:13 0
pTYB2-eIF1
 
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RRID:Addgene_37217 eIF1 Saccharomyces cerevisiae Ampicillin PMID:17913637 Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
pTYB2-eIF5
 
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RRID:Addgene_37219 eIF5 Saccharomyces cerevisiae Ampicillin PMID:17913637 Backbone Marker:NEB; Backbone Size:7474; Vector Backbone:pTYB2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
mZO1-1 shRNA
 
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RRID:Addgene_37215 mouse ZO-1 shRNA Mus musculus Ampicillin pLL5.0 derived from pLL3.7; CMV promoter replaced with 5' LTR promoter from pMSCV. Backbone Size:7700; Vector Backbone:pLL5.0; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 1
mZO1-2 shRNA
 
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RRID:Addgene_37216 mouse ZO-1 shRNA Mus musculus Ampicillin pLL5.0 derived from pLL3.7; CMV promoter replaced with 5' LTR promoter from pMSCV. Backbone Size:7700; Vector Backbone:pLL5.0; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
UQ6106
 
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RRID:Addgene_37176 None Ampicillin This strain contains Roberts lab plasmid pUX3236 which is pUX2424 (contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1– intergenic region – coxM1′->) – SalI]) but with a higher-affinity “e↑” binding site (5′-TCCTACAGTTCACGCACGT-3′). Suitable template for amplification and mutagenesis. For strain usage see attached pdf. Please note that this strain has not been confirmed by Addgene functionally. Vector Backbone:pUX3236; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
ZO-2 shRNA 1320
 
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RRID:Addgene_37210 dog ZO-2 shRNA canine Ampicillin PMID:19605556 Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
ZO-2 shRNA 2434
 
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RRID:Addgene_37211 dog ZO-2 shRNA canine Ampicillin PMID:19605556 Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
UQ5784
 
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RRID:Addgene_37173 ~5-kb BamHI + XhoI fragment from pUX2996 cloned into BamHI + SalI-cut pEXT20 Ampicillin This strain contains Roberts lab plasmid pUX3025 which contains ~5-kb BamHI + XhoI fragment from pUX2996 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “e + f” binding region. For strain usage details see attached pdf. Please note that this strain has not been tested functionally by Addgene. Vector Backbone:pUX3025; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
UQ5828
 
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RRID:Addgene_37175 ~5-kb BamHI + XhoI fragment from pUX3010 cloned into BamHI + SalI-cut pEXT20 Ampicillin This strain contains Roberts lab plasmid pUX3057 which contains ~5-kb BamHI + XhoI fragment from pUX3010 cloned into BamHI + SalI-cut pEXT20. Useful for mutagenesis and excision of the “a-f” binding region. For strain usage details see attached pdf. Please note that Addgene has not tested this strain functionally. Vector Backbone:pUX3057; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
ZO-1 shRNA 3253
 
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1+ mentions
RRID:Addgene_37206 dog ZO-1 shRNA canine Ampicillin PMID:19605556 Backbone Size:3176; Vector Backbone:pSUPER; Vector Types:Mammalian Expression, RNAi; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 1
pDonor MCS Rosa26
 
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10+ mentions
RRID:Addgene_37200 Rosa26 left targeting arm Mus musculus Ampicillin PMID:22169954 The homology arms in the ROSA26 donor vector were generated by PCR of mouse genomic DNA and insertion of the PCR-amplified right homology arm (left primer: 5-TTATTGCGGCCGCAG ATGGGCGGGAGTCTTCT-3, right primer: 5-AACA CCGCGGCAGTTTATAAATGGAGAAAAAGGAGA -3) between the NotI and SacII sites and the left homology arm (left primer: 5-TCTATGGTACCAGTTAACGGCA GCCGGAGT-3 , right primer: 5 -CGGACGAATTCTCT AGAAAGACTGGAGTTGCAGA-3) between the KpnI and EcoRI sites in the pZDonor AAVS1 vector obtained from Sigma–Aldrich. Backbone Marker:Sigma; Vector Backbone:pZDonor AAVS1; Vector Types:Genomic targeting - zinc finger; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 10
UQ4884
 
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RRID:Addgene_37161 rcoM2 in pEXT20 Burkholderia xenovorans Ampicillin PMID:18326575 This strain contains Roberts lab plasmid pUX2397 which contains an EcoRI-HindIII fragment bearing Burkholderia xenovorans LB400 rcoM2 cloned into pEXT20. Suitable for expression of Burkholderia xenovorans RcoM2 protein (untagged). Host strain is E. coli VJS6737. For strain usage see attached pdf. Vector Backbone:pEXT20; Vector Types:; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0

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