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Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191621 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191627 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191625 Copy
Species: E. coli
Genetic Insert: lacZ
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191629 Copy
Species: Eggerthella lenta
Genetic Insert: Dopamine dehydroxylase
Vector Backbone Description: Vector Backbone:pXD69m2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36494336
Proper citation: RRID:Addgene_191630 Copy
Species: Homo sapiens
Genetic Insert: hPol eta
Vector Backbone Description: Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36624150
Proper citation: RRID:Addgene_193968 Copy
Species: S. pyogenes
Genetic Insert: Cas9
Vector Backbone Description: Backbone Size:7662; Vector Backbone:pPMQAK1; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:35969224
Proper citation: RRID:Addgene_190790 Copy
Species: Mus musculus
Genetic Insert: TCR beta cDNA clone HSV2.3
Vector Backbone Description: Backbone Marker:Invitrogen ; Backbone Size:3947; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:11940116
Comments: This plasmid contains the TCR beta chain cDNA from the HSV2.3 clone and was used to create the transgenic construct p3A9cbetaTCR.HSV2.3
(Addgene plasmid #69594).
Proper citation: RRID:Addgene_74077 Copy
Species: Synthetic
Genetic Insert: aphII
Vector Backbone Description: Backbone Marker:Alexeyev, M.F., BioTechniques 18:52-54, 1995; Vector Backbone:pBSL15; Vector Types:Bacterial vector based on pMB1 replicon; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26921431
Proper citation: RRID:Addgene_74108 Copy
Species: Synthetic
Genetic Insert: Tol2-kanaR-cryaa-CFP
Vector Backbone Description: Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26818072
Proper citation: RRID:Addgene_74151 Copy
Species: Danio rerio
Genetic Insert: tia1l-sgRNA target site-hsp70-EGFP
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3931; Vector Backbone:pCR2.1-TOPO; Vector Types:CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36050398
Comments: The tia1l sgRNA was designed and published by Hwang et al., 2013, Nature Biotechnology: https://pubmed.ncbi.nlm.nih.gov/23360964/.
The strategy is based on a method described by Lackner et al. 2015, Nature Communications: https://pubmed.ncbi.nlm.nih.gov/26674669/.
Proper citation: RRID:Addgene_177837 Copy
Species: Synthetic
Genetic Insert: gRNA PCR template
Vector Backbone Description: Backbone Marker:TransGen Biotech; Vector Backbone:pEASY-blunt; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29703785
Comments: These plasmids are used for a cloning-free method of CRISPR/Cas9-mediated genome editing in fission yeast. This method takes advantage of gap repair in fission yeast cells to assemble two linear DNA fragments, a gapped Cas9-encoding plasmid and a PCR-amplified gRNA insert, into a circular plasmid. To reduce non-specific gap repair background, we adopt a split-marker strategy. pDB4280 and pDB4282 serve as the source of the gapped plasmid and the PCR template for gRNA insert amplification, respectively, in the split-ura4 system. pDB4281 and pDB4283 serve the same purposes for the split-bsdMX system. pDB4279 is a transformation control for the split-bsdMX system.
Proper citation: RRID:Addgene_98701 Copy
Species: Synthetic
Genetic Insert: gRNA PCR template
Vector Backbone Description: Backbone Marker:TransGen Biotech; Vector Backbone:pEASY-blunt; Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29703785
Comments: These plasmids are used for a cloning-free method of CRISPR/Cas9-mediated genome editing in fission yeast. This method takes advantage of gap repair in fission yeast cells to assemble two linear DNA fragments, a gapped Cas9-encoding plasmid and a PCR-amplified gRNA insert, into a circular plasmid. To reduce non-specific gap repair background, we adopt a split-marker strategy. pDB4280 and pDB4282 serve as the source of the gapped plasmid and the PCR template for gRNA insert amplification, respectively, in the split-ura4 system. pDB4281 and pDB4283 serve the same purposes for the split-bsdMX system. pDB4279 is a transformation control for the split-bsdMX system.
Proper citation: RRID:Addgene_98702 Copy
Species: Schmidtea mediterranea
Genetic Insert: mblk in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-CGTGCTCGTGAGAATTGTTG, R-AGTACGCAGCAGAGGCAAGT
Proper citation: RRID:Addgene_99091 Copy
Species: Schmidtea mediterranea
Genetic Insert: GluR1 (Glutamate receptor 1) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-GTTATCGGAAGCGACGACAT, R-TGGATGACACCGAGCTATTG
Proper citation: RRID:Addgene_99070 Copy
Species: Schmidtea mediterranea
Genetic Insert: Ndk (nou darake) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-atcgacatatgacaataccgattgtcaaacccttc, R-atcgaggatccttaggccttaactccaattatatc;also has Nde1/BamHI sites for subcloning
Proper citation: RRID:Addgene_99072 Copy
Species: Schmidtea mediterranea
Genetic Insert: Bmp4 (also known as bmp) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-CGGATTTTGGAGGAATAGGTC, R-TCTGGGCGTAAATTGTAGGG
Proper citation: RRID:Addgene_99076 Copy
Species: Schmidtea mediterranea
Genetic Insert: ActR1 (activin receptor 1) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-GCTGGCTCTTCAAACCAAGA, R-GGTAAACCTGGAATCGCTCA
Proper citation: RRID:Addgene_99077 Copy
Species: Schmidtea mediterranea
Genetic Insert: h.72.4d in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TTTTTGATTTGTAACTTTGTTCTTGA, R-CAATGAATCAATAGAATGTGGAAT
Proper citation: RRID:Addgene_99080 Copy
Species: Schmidtea mediterranea
Genetic Insert: MCP (also known as h.45.8g) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-CCAACAAGGCTTTTTATTATTTATG, R-GAAATGATGGCATAGGAGGTC
Proper citation: RRID:Addgene_99083 Copy
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