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pRK5RS-HERCD533
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65224 HERCD533 Homo sapiens Ampicillin PMID:1346334 Insert codes for AA 1-653 (extracellular and transmembrane domains) Note: Amino acid number may be off when compared to current NCBI entry. Please check QC sequence to verify end of coding region. Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin deleted AA 654-1186 2026-08-15 01:18:16 1
pYTK080
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65187 ZeocinR Synthetic Chloramphenicol PMID:25871405 Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:18:16 1
pDCAF3-IS5
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65220 ndmA Pseudomonas putida strain CBB5 Chloramphenicol and Kanamycin PMID:23654268 The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3. Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100). N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See: Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049. For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC). Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin 2026-08-15 01:18:17 1
pLXSN-Axl
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65222 AXL Homo sapiens Ampicillin PMID:18339872 Backbone Marker:Clontech; Backbone Size:5900; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin 2026-08-15 01:18:16 1
pYTK081
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65188 CEN6/ARS4 Saccharomyces cerevisiae Chloramphenicol PMID:25871405 Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol 2026-08-15 01:18:17 1
pYTK083
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65190 AmpR-ColE1 Synthetic Ampicillin PMID:25871405 Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:16 4
pYTK085
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65192 SpecR-ColE1 Synthetic Spectinomycin PMID:25871405 Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405. Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin 2026-08-15 01:18:16 2
pcDNA3-HA-ERK5
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65244 ERK5 Homo sapiens Ampicillin PMID:12042304 Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin A409T 2026-08-15 01:18:17 3
GFP-ATAD2
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65370 ATAD2 Homo sapiens Ampicillin PMID:25593309 Gene Source: OriGene RG218291 Backbone Marker:invitrogen; Backbone Size:5847; Vector Backbone:pcDNA6.2/N-EmGFP-DEST ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin none 2026-08-15 01:18:19 2
Ii-Str_puromycin
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65309 Invariant chain (p33) Homo sapiens Ampicillin PMID:22406856 luminal tagging Backbone Marker:Clontech; Vector Backbone:pIRESpuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Streptavidin in its lumenal part 2026-08-15 01:18:17 1
Ii-Str_neomycin
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65308 Invariant chain (p33) Homo sapiens Ampicillin PMID:22406856 luminal tagging Depositor states that H293Q mutation within Ii insert does not affect plasmid function. Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin Streptavidin in its lumenal part 2026-08-15 01:18:17 1
pCDH_Str-KDEL_neomycin
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65307 Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end Other Ampicillin PMID:22406856 luminal tagging Backbone Marker:system biosciences; Vector Backbone:pCDH-CMV-MCS-EF1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:18:18 3
Str-KDEL_neomycin
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65306 Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end Other Ampicillin PMID:22406856 luminal tagging Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:17 3
Str-STIM1-NN_ST-SBP-EGFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65262 Str-STIM1-NN and truncated sialyltransferase Homo sapiens Ampicillin PMID:22406856 luminal tagging Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin contains only amino acids 1 to 43 of ST 2026-08-15 01:18:17 2
Str-Ii_VSVG-SBP-EGFP
 
Resource Report
Resource Website
10+ mentions 		RRID:Addgene_65300 VSV-G Other Ampicillin PMID:22406856 cytoplasmic tagging Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:17 20
Str-STIM1-NN_ST-SBP-mCherry
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65263 Str-STIM1-NN and truncated sialyltransferase Homo sapiens Ampicillin PMID:22406856 luminal tagging Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin contains only amino acids 1 to 43 of ST 2026-08-15 01:18:18 1
Str-KDEL_ST-SBP-mCherry
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65265 Str-KDEL and truncated sialyltransferase Homo sapiens Ampicillin PMID:22406856 luminal tagging Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin contains only amino acids 1 to 43 of ST 2026-08-15 01:18:17 4
pCDH_Str-Ii
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65313 Invariant chain (p33) Homo sapiens Ampicillin PMID:22406856 cytoplasmic tagging Backbone Marker:system biosciences; Vector Backbone:pCDH-CMV-MCS-EF1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin Streptavidin in its cytoplasmic part 2026-08-15 01:18:18 2
Ii-Str_TNF-SBP-EGFP
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65280 TNF Homo sapiens Kanamycin PMID:22406856 luminal tagging Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin 2026-08-15 01:18:17 2
TNF-SBP-mCherry_puromycin
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_65285 TNF Homo sapiens Ampicillin PMID:22406856 luminal tagging Backbone Marker:Clontech; Vector Backbone:pIRESpuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:18:17 1

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