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Species: Homo sapiens
Genetic Insert: HERCD533
Vector Backbone Description: Backbone Marker:BD PharMingen; Backbone Size:4754; Vector Backbone:pRK5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1346334
Comments: Insert codes for AA 1-653 (extracellular and transmembrane domains) Note: Amino acid number may be off when compared to current NCBI entry. Please check QC sequence to verify end of coding region.
Proper citation: RRID:Addgene_65224 Copy
Species: Synthetic
Genetic Insert: ZeocinR
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 6 parts are Yeast Markers in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65187 Copy
Species: Pseudomonas putida strain CBB5
Genetic Insert: ndmA
Vector Backbone Description: Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23654268
Comments: The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3.
Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100).
N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See:
Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049.
For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC).
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_65220 Copy
Species: Homo sapiens
Genetic Insert: AXL
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5900; Vector Backbone:pLXSN; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18339872
Proper citation: RRID:Addgene_65222 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CEN6/ARS4
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65188 Copy
Species: Synthetic
Genetic Insert: AmpR-ColE1
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65190 Copy
Species: Synthetic
Genetic Insert: SpecR-ColE1
Vector Backbone Description: Vector Backbone:self-made; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25871405
Comments: Type 7 and 8 parts are Yeast propagation machinery in the Dueber YTK. For more information, please visit the supplemental information in PMID:25871405.
Proper citation: RRID:Addgene_65192 Copy
Species: Homo sapiens
Genetic Insert: ERK5
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12042304
Proper citation: RRID:Addgene_65244 Copy
Species: Homo sapiens
Genetic Insert: ATAD2
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5847; Vector Backbone:pcDNA6.2/N-EmGFP-DEST ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25593309
Comments: Gene Source: OriGene RG218291
Proper citation: RRID:Addgene_65370 Copy
Species: Homo sapiens
Genetic Insert: Invariant chain (p33)
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESpuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65309 Copy
Species: Homo sapiens
Genetic Insert: Invariant chain (p33)
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Depositor states that H293Q mutation within Ii insert does not affect plasmid function.
Proper citation: RRID:Addgene_65308 Copy
Species: Other
Genetic Insert: Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
Vector Backbone Description: Backbone Marker:system biosciences; Vector Backbone:pCDH-CMV-MCS-EF1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65307 Copy
Species: Other
Genetic Insert: Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65306 Copy
Species: Homo sapiens
Genetic Insert: Str-STIM1-NN and truncated sialyltransferase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65262 Copy
Species: Other
Genetic Insert: VSV-G
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: cytoplasmic tagging
Proper citation: RRID:Addgene_65300 Copy
Species: Homo sapiens
Genetic Insert: Str-STIM1-NN and truncated sialyltransferase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65263 Copy
Species: Homo sapiens
Genetic Insert: Str-KDEL and truncated sialyltransferase
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65265 Copy
Species: Homo sapiens
Genetic Insert: Invariant chain (p33)
Vector Backbone Description: Backbone Marker:system biosciences; Vector Backbone:pCDH-CMV-MCS-EF1-puro; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: cytoplasmic tagging
Proper citation: RRID:Addgene_65313 Copy
Species: Homo sapiens
Genetic Insert: TNF
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESneo3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65280 Copy
Species: Homo sapiens
Genetic Insert: TNF
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pIRESpuro3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22406856
Comments: luminal tagging
Proper citation: RRID:Addgene_65285 Copy
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