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Plasmids are provided by Addgene and DGRC.

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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
EYFP-Abr NE/AA
 
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RRID:Addgene_36419 active BCR-related Homo sapiens Kanamycin PMID:17116687 Made by direct mutagenesis of EYFP-Abr using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N245/E246 in Abr. We therefore mutated these residues in Abr and introduced the mutant into the RAW 264.7 macrophage cell line. However, the construct’s expression did not cause morphological changes in these cells. constructed by Young-Jin Cho. Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin N245A, E246A 2026-08-15 01:14:10 0
ECFP-Bcr NE/AA
 
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1+ mentions
RRID:Addgene_36418 breakpoint cluster region Homo sapiens Kanamycin PMID:17116687 Made by mutagenesis directly on plasmid Bcr-ECFP using a Stratagene Quick Change kit. In Dbl, mutation of residues N673 and D674 completely abolished its activity as a GEF towards Rac. These residues are highly conserved among DH domains and are also conserved as residues N689/ E690 in Bcr. We therefore mutated these residues in Bcr and introduced the mutants into the RAW 264.7 macrophage cell line. However, the construct did not caused morphological changes in these cells. Constructed by Young-Jin Cho. Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin N689A/ E690A 2026-08-15 01:14:08 1
Hox D4a
 
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RRID:Addgene_36469 Hox D4a Danio rerio Ampicillin PMID:9425136 Please note that Addgene's sequencing results indicate the Hox D4a insert in this plasmid has Q100H, A101T, G125C and R189W mutations when compared to GenBank entry NP_001119917.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication. Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression, Riboprobe Synthesis; Bacterial Resistance:Ampicillin Q100H, A101T, G125C and R189W 2026-08-15 01:14:10 0
Hox D13a
 
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RRID:Addgene_36467 hoxa11b Danio rerio Ampicillin PMID:18638469 Please note that Addgene's sequencing results indicate the Hox D13a insert in this plasmid has F63I, P75Q and M133I mutations when compared to GenBank entry AAI53653.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication. Backbone Marker:Promega; Backbone Size:3000; Vector Backbone:pGEM-T; Vector Types:Riboprobe Synthesis; Bacterial Resistance:Ampicillin F63I, P75Q and M133I 2026-08-15 01:14:08 0
pGEX6P1_EHD1_438-534
 
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1+ mentions
RRID:Addgene_36459 EHD1 EH domain Homo sapiens Ampicillin Backbone Marker:GE Healthcare; Backbone Size:4984; Vector Backbone:pGEX6P1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin deleted amino acids 1-437 from full-length construct 2026-08-15 01:14:08 1
pNRB65
 
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RRID:Addgene_36455 ARS501-LEU2 Saccharomyces cerevisiae Ampicillin This plasmid is for generating dmc1Δ::LEU2 alleles by single step transplacement. See the attached map for additional details. Backbone Size:3000; Vector Backbone:pNRB8; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:08 0
TAL2254
 
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RRID:Addgene_36697 FANCC-TALEN_Left Homo sapiens Ampicillin PMID:22484455 Target binding site: TGAAGGGACATCACCTTT It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32290); Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:09 0
pEGFP-C1-hAtg2A
 
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1+ mentions
RRID:Addgene_36456 autophagy-related protein 2 homolog A Homo sapiens Kanamycin PMID:22219374 Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin changed Proline 656 to Arginine 2026-08-15 01:14:10 7
TAL2260
 
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RRID:Addgene_36699 BMPR1A-TALEN_Left Homo sapiens Ampicillin PMID:22484455 Target binding site: TACAATTGAACAATGCCT It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32290); Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:09 0
TAL2252
 
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RRID:Addgene_36695 EWSR1-TALEN_Left Homo sapiens Ampicillin PMID:22484455 Target binding site: TGGCGTCCACGGGTGAGT It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32290); Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:10 0
TAL2239
 
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RRID:Addgene_36690 CHD7-TALEN_Right Homo sapiens Ampicillin PMID:22484455 Target binding site: TCCTCGCCAAAAAGACTC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:10 0
TAL2246
 
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RRID:Addgene_36691 PHF8-TALEN_Left Homo sapiens Ampicillin PMID:22484455 Target binding site: TGAGTACTCCGCCTCTAC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:09 0
TAL2238
 
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RRID:Addgene_36689 CHD7-TALEN_Left Homo sapiens Ampicillin PMID:22484455 Target binding site: TGTGTTGGAAGAAGATGG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:09 0
TAL2287
 
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RRID:Addgene_36722 HOXC13-TALEN_Right Homo sapiens Ampicillin PMID:22484455 Target binding site: TCCGGCCAGCGTGGATGC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:10 0
TAL2288
 
Resource Report
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RRID:Addgene_36723 FANCG-TALEN_Left Homo sapiens Ampicillin PMID:22484455 Target binding site: TCGGCCACCATGTCCC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:09 0
pHAGE hSPC-dsRed Express-W
 
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RRID:Addgene_36449 human SP-C promoter Homo sapiens Ampicillin PMID:22482505 Cloned h3.7kb SP-C promoter (from Whitsett 3.7SPC/SV40 plasmid) into pHAGE-CMV-GFP-W as follows: SV40t removed from SPC plasmid by BamH1-BamH1 cutting, blunting and self-ligtion of blunt ends. Entire hSPC promoter lifted out by cutting Nde1-Not1 and ligated into pHAGE CMV-GFP-W backbone pre-cut Nde1-Not1 to remove most of CMV promoter. Cut this backbone Spe1-Nde1 to remove residual CMV promoter fragment, blunted and ligated NB: final vector still has Spe1 site at start of promoter. Backbone Marker:Richard Mulligan; Backbone Size:4874; Vector Backbone:pHAGE; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:08 0
TAL2231
 
Resource Report
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RRID:Addgene_36686 SDHD-TALEN_Right Homo sapiens Ampicillin PMID:22484455 Target binding site: TAGGGCACCGCAAACGGC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:09 0
TAL2285
 
Resource Report
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RRID:Addgene_36720 NCOR2-TALEN_Right Homo sapiens Ampicillin PMID:22484455 Target binding site: TGGGTAGGAAAGGCTG It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32288); Backbone Size:6447; Vector Backbone:JDS74; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:09 0
TAL2237
 
Resource Report
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RRID:Addgene_36688 CHD4-TALEN_Right Homo sapiens Ampicillin PMID:22484455 Target binding site: TCCTCCTCACTGCCCGCC It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32287); Backbone Size:6447; Vector Backbone:JDS71; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:09 0
TAL2286
 
Resource Report
Resource Website
RRID:Addgene_36721 HOXC13-TALEN_Left Homo sapiens Ampicillin PMID:22484455 Target binding site: TCCAGCAGATCATGTCAT It is strongly recommended that users perform a diagnostic digest to verify this plasmid prior to use. For example, a double digest of the plasmid with BamHI and KpnI should result in two bands at 2.2kb and 5.9kb. Backbone Marker:Joung lab (Addgene Plasmid # 32290); Backbone Size:6447; Vector Backbone:JDS78; Vector Types:Mammalian, T7 for in vitro mRNA synthesis; Bacterial Resistance:Ampicillin 2026-08-15 01:14:09 0

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