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Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Vector Backbone:pDR175; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_98913 Copy
Species: E. coli
Genetic Insert: FtsZ
Vector Backbone Description: Vector Backbone:pCA24N; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25848771
Proper citation: RRID:Addgene_98914 Copy
Species: E. coli
Genetic Insert: Hfq
Vector Backbone Description: Backbone Size:5100; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29140461
Proper citation: RRID:Addgene_174661 Copy
Species: E. coli
Genetic Insert: rpoD
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Addgene NGS found a N149D amino acid variation in the rpoD translation, that the depositing laboratory confirms to be a naturally occurring sequence variant. This will not adversely affect plasmid function.
Please visit https://www.biorxiv.org/content/10.1101/2020.02.17.953182v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_129688 Copy
Species: E. coli
Genetic Insert: codon optimized S1 subunit of OZITX
Vector Backbone Description: Vector Backbone:pcDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35322196
Proper citation: RRID:Addgene_184925 Copy
Species: E. coli
Genetic Insert: Scrambled sgRNA targeting sequences
Vector Backbone Description: Vector Backbone:L4440_BioBrick-sgRNA; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35636511
Proper citation: RRID:Addgene_177811 Copy
Species: E. coli
Genetic Insert: None
Vector Backbone Description: Vector Backbone:N/A; Vector Types:; Bacterial Resistance:None
Defining Citation: PMID:31772280
Comments: Genotype: λDE3 Δtig100 Δ(araD–araB)567 ΔlacZ4787(::rrnB-3) lacIP-4000(lacIQ) rph-1 Δ(rhaD–rhaB)568 hsdR514
KTD101(DE3) is a λDE3 derivative of the trigger factor deficient (Δtig) strain KTD101 from Puertas et al 2010 Protein Expression and Purification 74: 122–128, PMID 20600941.
Puertas et al 2010 showed that deletion of trigger factor in the parent strain KTD101 increased the level of secreted recombinantly expressed protein. This strain may also improve the level of protein expression in the periplasm and in the outer membrane. This derivative strain KTD101(DE3) now allows expression that is based on the widely-used T7 promoter.
The parent strain KTD101 was provided by François Baneyx at the University of Washington.
DE3 lysogenization is apparent from positive expression that was obtained for the BBA57 protein upon expression from the T7 promoter plasmid pBBA57-TEV-His12, as was shown in Figure S5C, lane 7, of Robertson et al, Sci Rep. 2019 Nov 26;9(1):17606. doi: 10.1038/s41598-019-53830-x.
Proper citation: RRID:Addgene_138651 Copy
Species: E. coli
Genetic Insert: PPK
Vector Backbone Description: Vector Backbone:p416; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35862758
Proper citation: RRID:Addgene_183943 Copy
Species: E. coli
Genetic Insert: PPK
Vector Backbone Description: Vector Backbone:pRS415; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35862758
Proper citation: RRID:Addgene_183941 Copy
Species: E. coli
Genetic Insert: anti rabbit secondary nanobody fused to Tn5
Vector Backbone Description: Vector Backbone:pET-29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36536148
Comments: Please visit https://doi.org/10.1101/2022.03.08.483459 for bioRxiv preprint.
Proper citation: RRID:Addgene_183638 Copy
Species: E. coli
Genetic Insert: ispA
Vector Backbone Description: Backbone Marker:F. X. Cunningham Jr.; Backbone Size:9259; Vector Backbone:pAC-BETAipi; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34533983
Proper citation: RRID:Addgene_178302 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184962 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184982 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184981 Copy
Species: E. coli
Genetic Insert: Eco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pET-21(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184960 Copy
Species: E. coli
Genetic Insert: Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184966 Copy
Species: E. coli
Genetic Insert: Integrating inducible cassette: Eco1 RT
Vector Backbone Description: Vector Backbone:pZS157 (Addgene plasmid #114454); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184970 Copy
Species: E. coli
Genetic Insert: Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
Vector Backbone Description: Vector Backbone:pZS165 (Addgene plasmid #114451); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184979 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Backbone Marker:System Biosciences; Backbone Size:6892; Vector Backbone:PB-EF1α-MCS-IRES-Neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35058555
Proper citation: RRID:Addgene_179510 Copy
Species: E. coli
Genetic Insert: Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
Vector Backbone Description: Vector Backbone:pFUGW-H1 (Addgene plasmid #25870); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34949838
Proper citation: RRID:Addgene_184998 Copy
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