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Plasmids are provided by Addgene and DGRC.

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On page 53 showing 1041 ~ 1060 out of 1,969 results
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  • RRID:Addgene_99082

http://www.addgene.org/99082

Species: Schmidtea mediterranea
Genetic Insert: nemo-like kinase (also known as h.109.4e) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-CAAATGGTTGTTACGCTCCA R-GACATGGTCGACTGACAACG

Proper citation: RRID:Addgene_99082 Copy   


  • RRID:Addgene_99084

http://www.addgene.org/99084

Species: Schmidtea mediterranea
Genetic Insert: Unc-9 (also known as h.26.12f) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-TGTAGCTTTTTCCGGGAATTT, R-TACAATGCGTATTGCCGTTG

Proper citation: RRID:Addgene_99084 Copy   


  • RRID:Addgene_99089

http://www.addgene.org/99089

Species: Schmidtea mediterranea
Genetic Insert: Runt-1 (also known as runt) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-AGTCGCAGTGGAAGAGGAAA, R-TGATGGGATTGCGAGTTGTA

Proper citation: RRID:Addgene_99089 Copy   


  • RRID:Addgene_99067

http://www.addgene.org/99067

Species: Schmidtea mediterranea
Genetic Insert: notum in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Note: The notum insert contains several mismatches compared to JF725701.1. The depositor has confirmed this does not affect plasmid function.

Proper citation: RRID:Addgene_99067 Copy   


  • RRID:Addgene_99069

http://www.addgene.org/99069

Species: Schmidtea mediterranea
Genetic Insert: ChAT (choline acetyltransferase) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-GATCTCCTGCCACCAAAGAA, R-AACTCCATGACCCTGAATCG

Proper citation: RRID:Addgene_99069 Copy   


  • RRID:Addgene_99068

http://www.addgene.org/99068

Species: Schmidtea mediterranea
Genetic Insert: foxD in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23297191
Comments: Cloning primers: F-CCAGGCGTTCAAAGATTCTC, R-GATTTCCCGGTTCTCTTGGT

Proper citation: RRID:Addgene_99068 Copy   


  • RRID:Addgene_99093

http://www.addgene.org/99093

Species: Schmidtea mediterranea
Genetic Insert: LDLRR-1 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-GCCTCACAATAGCACTTTCG R-GTAAATATTGATAATATATTTAAT Note: There is a 1.3 kb deletion in the LDLRR-1 insert compared to KY024334.1. The depositor has confirmed this does not affect plasmid function.

Proper citation: RRID:Addgene_99093 Copy   


  • RRID:Addgene_99096

http://www.addgene.org/99096

Species: Schmidtea mediterranea
Genetic Insert: F-spondin in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-AAACAAAAGAGCATGGTTCA, R-AATCTTGCAAACATTCGAGA Note: Only 143 base pairs from the insert align with KY024329.1. The depositor has confirmed this does not impact plasmid function.

Proper citation: RRID:Addgene_99096 Copy   


  • RRID:Addgene_99098

http://www.addgene.org/99098

Species: Schmidtea mediterranea
Genetic Insert: CNG1 in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-AATTGTCGAGCCCTTGGTAG, R-CCCAAATTTTTCGTTTAAAGGTT

Proper citation: RRID:Addgene_99098 Copy   


  • RRID:Addgene_99102

http://www.addgene.org/99102

Species: Schmidtea mediterranea
Genetic Insert: slc1a-5 (also known as excitatory amino acid transporter) in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-gtcaaatgggaaaggaagca R-attaattgtggcgcctatcg

Proper citation: RRID:Addgene_99102 Copy   


  • RRID:Addgene_99104

http://www.addgene.org/99104

Species: Schmidtea mediterranea
Genetic Insert: estrella in situ probe
Vector Backbone Description: Backbone Marker:Phillip Newmark (Addgene plasmid # 26536); Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27612384
Comments: Cloning primers: F-GAAGTCAATGGAACAGACGA, R-GAATTAGTGAGCGACCAGAA Note: The estrella insert contains several mismatches compared to KY024338.1. The depositor has confirmed this does not affect plasmid function.

Proper citation: RRID:Addgene_99104 Copy   


  • RRID:Addgene_103148

http://www.addgene.org/103148

Species: Homo sapiens
Genetic Insert: hsa-let-7b-5p target
Vector Backbone Description: Vector Backbone:Low Sensor Backbone (LSB); Vector Types:Mammalian Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:29934631
Comments: Please note that this plasmid is inherently unstable due to a repeating poly A element. We recommend performing a diagnostic digest on multiple colonies upon receipt. The depositors would like to note the presence of several discrepancies between the depositor sequence and physical plasmid sequence. In particular, the sequences upstream and downstream of the puromycin coding region have 10 bp insertions which do not affect the ability to perform puromycin selection. The sequences for those regions within the physical plasmids are as follows: Upstream of Puro: GAATTCGACCGCCTGTCTCAAGGTGCCACC; Downstream of Puro: GCTTTGAGACTACGCGTGAATTCACTCCTC.

Proper citation: RRID:Addgene_103148 Copy   


  • RRID:Addgene_190785

http://www.addgene.org/190785

Species: Streptomyces lavendulae subsp. lavendulae CCM 3239
Genetic Insert: T5 terminator
Vector Backbone Description: Backbone Size:4065; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36173451

Proper citation: RRID:Addgene_190785 Copy   


  • RRID:Addgene_190786

http://www.addgene.org/190786

Species: pSA3239
Genetic Insert: upstream region of the aur1 cluster for auricin
Vector Backbone Description: Backbone Size:7907; Vector Backbone:sCos-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36173451

Proper citation: RRID:Addgene_190786 Copy   


  • RRID:Addgene_92081

http://www.addgene.org/92081

Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Enzynomics, Korea; Vector Backbone:pTOP-V2; Vector Types: tagging proteins in Cryptococcus neoforman; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958

Proper citation: RRID:Addgene_92081 Copy   


  • RRID:Addgene_92085

http://www.addgene.org/92085

Species: Other
Genetic Insert: GFP
Vector Backbone Description: Backbone Marker:Enzynomics, Korea; Vector Backbone:pTOP-V2; Vector Types: tagging proteins in Cryptococcus neoforman; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958

Proper citation: RRID:Addgene_92085 Copy   


http://www.addgene.org/92086

Species: Other
Genetic Insert: 4xFlag
Vector Backbone Description: Backbone Marker:Enzynomics, Korea; Vector Backbone:pTOP-V2; Vector Types: tagging proteins in Cryptococcus neoforman; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958

Proper citation: RRID:Addgene_92086 Copy   


  • RRID:Addgene_92083

http://www.addgene.org/92083

Species: Other
Genetic Insert: 6xHA
Vector Backbone Description: Backbone Marker:Enzynomics, Korea; Vector Backbone:pTOP-V2; Vector Types: tagging proteins in Cryptococcus neoforman; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958

Proper citation: RRID:Addgene_92083 Copy   


http://www.addgene.org/92084

Species: Other
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Enzynomics, Korea; Vector Backbone:pTOP-V2; Vector Types: tagging proteins in Cryptococcus neoforman; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958
Comments: Addgene's sequencing results found K128R and Q193P mutations in mCherry. These mutations are not known to affect plasmid function.

Proper citation: RRID:Addgene_92084 Copy   


http://www.addgene.org/92082

Species: Other
Genetic Insert: 4xFlag
Vector Backbone Description: Backbone Marker:Enzynomics, Korea; Vector Backbone:pTOP-V2; Vector Types: tagging proteins in Cryptococcus neoforman; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:27780958

Proper citation: RRID:Addgene_92082 Copy   



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