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Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105848 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105846 Copy
Species: E. coli
Genetic Insert: hydroperoxidase II catalase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBad; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Please visit https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/838 for Chemical Biology & Biochemistry Laboratory Using Genetic Code Expansion Manual.
Proper citation: RRID:Addgene_105847 Copy
Species: E. coli
Genetic Insert: eatA[H134R]
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4126; Vector Backbone:pBAD-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_168195 Copy
Species: E. coli
Genetic Insert: eatA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4127; Vector Backbone:pBAD-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:14977988
Proper citation: RRID:Addgene_168224 Copy
Species: E. coli
Genetic Insert: Lipocalin Blc
Vector Backbone Description: Backbone Size:3958; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33210733
Proper citation: RRID:Addgene_168255 Copy
Species: E. coli
Genetic Insert: EGFP-eDHFR-20aa
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4710; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23941503
Proper citation: RRID:Addgene_172108 Copy
Species: E. coli
Genetic Insert: RepX superhelicase
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:25883358
Comments: Please see https://pubmed.ncbi.nlm.nih.gov/33657402/ for additional information
Proper citation: RRID:Addgene_172199 Copy
Species: E. coli
Genetic Insert: Peptidyl prolyl isomerase domain 1 of SurA
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:7280; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17825319
Proper citation: RRID:Addgene_15934 Copy
Species: E. coli
Genetic Insert: ccdB
Vector Backbone Description: Backbone Size:6064; Vector Backbone:ptac; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:16705187
Proper citation: RRID:Addgene_15922 Copy
Species: E. coli
Genetic Insert: EF2n_mFAP2b
Vector Backbone Description: Vector Backbone:pET15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159468 Copy
Species: E. coli
Genetic Insert: EF1n_mFAP2b
Vector Backbone Description: Vector Backbone:pET15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159473 Copy
Species: E. coli
Genetic Insert: EF1p3_mFAP2b
Vector Backbone Description: Vector Backbone:pET15; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_159470 Copy
Species: E. coli
Genetic Insert: surA
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:7280; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17825319
Comments: There is an additional mutation in this plasmid - M136I. Depositing scientist does not think it has any effect on protein function.
Proper citation: RRID:Addgene_15957 Copy
Species: E. coli
Genetic Insert: mature SurA protein M231R mutant
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:7280; Vector Backbone:pTYB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17825319
Comments: There is an additional mutation in this plasmid - M136I. Depositing scientist does not think it has any effect on protein function.
Proper citation: RRID:Addgene_15956 Copy
Species: E. coli
Genetic Insert: AdEasier-1 bacterial cells
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Ampicillin and Streptomycin
Defining Citation: PMID:9482916
Comments: AdEasier-1 cells are BJ5183 derivatives which already contain the AdEasy-1 plasmid. Please note that you cannot isolate AdEasy-1 from this strain. Please see Plasmid #16400 if you only want the plasmid. In order to generate recombinant vectors ready to transfer into 293 cells, one simply has to transform the AdEasier-1 cells with a shuttle vector (such as pAdTrack-CMV) containing the gene of interest, and then proceed as with the standard AdEasy system.
AdEasier is a trademark of the Johns Hopkins University.
Proper citation: RRID:Addgene_16399 Copy
Species: E. coli
Genetic Insert: BJ5183 bacterial cells
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:9482916
Comments: This is a bacterial strain - not a plasmid. Select with 30 ug/mL streptomycin.
Electrocompetent BJ5183 cells are used to carry out the recombination between the shuttle vector (pShuttle, pShuttle-CMV, pAdTrack, or pAdTrack-CMV) and the adenoviral vector (pAdEasy1 or pAdEasy2).
Proper citation: RRID:Addgene_16398 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164596 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164598 Copy
Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164600 Copy
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