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Species: Danio rerio
Genetic Insert: -2.1prox1a
Vector Backbone Description: Backbone Marker:Bessa et al, 2009 (PMID 19653328); Vector Backbone:ZED_vector; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:38722096
Comments: Please visit https://doi.org/10.1101/2023.08.21.550483 for bioRxiv preprint.
Proper citation: RRID:Addgene_218205 Copy
Species: Danio rerio
Genetic Insert: Unc-503 promoter
Vector Backbone Description: Vector Backbone:pDONRP4-P1r; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:41230856
Comments: Please visit https://doi.org/10.1101/2024.07.13.603267 for bioRxiv preprint.
Proper citation: RRID:Addgene_224482 Copy
Species: Danio rerio
Genetic Insert: Histone Deacetylase 10 humanized catalytic domain
Vector Backbone Description: Backbone Marker:Scott Gradia; Backbone Size:6465; Vector Backbone:pET28a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32659072
Proper citation: RRID:Addgene_225542 Copy
Species: Danio rerio
Genetic Insert: drAgo2DY-EF
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221716 Copy
Species: Danio rerio
Genetic Insert: drAgo2DY-EF
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221733 Copy
Species: Danio rerio
Genetic Insert: drAgo2
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221730 Copy
Species: Danio rerio
Genetic Insert: CBS pair domain of zebrafish CNNM3
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250563 Copy
Species: Danio rerio
Genetic Insert: CBS pair domain of zebrafish CNNM1
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250561 Copy
Species: Danio rerio
Genetic Insert: zebrafish PRL3
Vector Backbone Description: Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_250560 Copy
Species: Danio rerio
Genetic Insert: apoBb.1-Dendra2
Vector Backbone Description: Backbone Marker:Genewiz; Backbone Size:2626; Vector Backbone:pUC57-Kan; Vector Types:TALEN; Bacterial Resistance:Kanamycin
Defining Citation: PMID:40836916
Comments: On this plasmid encoded is the C-terminal sequence of apoBb.1 (zebrafish ortholog of APOB) spanning the region of the TALEN homology region. followed by an HA-tag, then by a 4-Gly linker, and then the Dendra2 sequence with a STOP at the end, followed by the UTR of apoBb1 which servers as TALEN homology region.
Proper citation: RRID:Addgene_218180 Copy
Species: Danio rerio
Genetic Insert: Acvr1ba + VfLOV domain and Acvr2ba + VfLOV domain
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40568079
Comments: Based on the constructs first developed by Sako et al. 2016 (Cell Reports, https://doi.org/10.1016/j.celrep.2016.06.036).
Please visit https://doi.org/10.1101/2025.04.17.649426 for bioRxiv preprint.
Proper citation: RRID:Addgene_232640 Copy
Species: Danio rerio
Genetic Insert: zFGFR1a kinase domain + VfLOV domain
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2+; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:40568079
Comments: Based on the chassis first developed by Sako et al. 2016 (Cell Reports, https://doi.org/10.1016/j.celrep.2016.06.036), and later used in Rogers et al. 2020 (eLife, https://doi.org/10.7554/elife.58641).
Please visit https://doi.org/10.1101/2025.04.17.649426 for bioRxiv preprint.
Proper citation: RRID:Addgene_232639 Copy
Species: Danio rerio
Genetic Insert: her3
Vector Backbone Description: Backbone Marker:Jason Berman (Addgene Plasmid #74668); Backbone Size:4915; Vector Backbone:pCS2+MCS-P2A-sfGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36696714
Proper citation: RRID:Addgene_240545 Copy
Species: Danio rerio
Genetic Insert: arrestin, beta 2a
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_251767 Copy
Species: Danio rerio
Genetic Insert: zrif1_ex8_LEFT
Vector Backbone Description: Vector Backbone:pCS2TAL3-DD; Vector Types:TALEN; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.21.512903v1 for preprint.
Proper citation: RRID:Addgene_194435 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Christian Mosimann; Backbone Size:4974; Vector Backbone:pTol2Dest_pCY; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Cloned by Maya Pahima.
nos2 control elements were cloned from zebrafish ovary gDNA.
Gateway recombination: pTol2-nos2L:d2egfp-nos2,3’utr, cryaa:Venus (pCY) was generated by combining p5E-nos2promL, pME-d2GFP (Collery and Link, 2011), and pCR4-nos2,3’utr (Chapter 6) into the pTol2-R4/R3_cryaa:Venus backbone vector (Mosimann et al., 2013).
Proper citation: RRID:Addgene_226801 Copy
Species: Danio rerio
Genetic Insert: Ace2N-mNeon
Vector Backbone Description: Vector Backbone:pDestTol pA2; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:42109300
Comments: Please visit https://doi.org/10.1101/2024.12.12.628143 for bioRxiv preprint.
Proper citation: RRID:Addgene_253618 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Florence Marlow, Amanda Heim; Backbone Size:6946; Vector Backbone:pTol2Dest_pBH; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Cloned by Maya Pahima.
pTol2-nos2L:mApple, cmlc2:dsred (pBH) was generated by combining p5E-nos2prom and pME-mApple (Addgene, 195960) (Kemmler et al., 2023) into the pTol2-R4/R2_cmlc2:dsred (pBH) backbone vector (Heim et al., 2014).
Proper citation: RRID:Addgene_226797 Copy
Species: Danio rerio
Genetic Insert: nanos2
Vector Backbone Description: Backbone Marker:Florence Marlow, Amanda Heim; Backbone Size:6946; Vector Backbone:pTol2Dest_pBH; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Comments: Cloned by Maya Pahima.
pTol2-nos2:emGFP, cmlc2:dsred (pBH) was generated by combining p5E-nos2prom and pME-emGFP into the pTol2-R4/R2_cmlc2:dsred (pBH) backbone vector (Heim et al., 2014).
Proper citation: RRID:Addgene_226798 Copy
Species: Danio rerio
Genetic Insert: kcnh5b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255308 Copy
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