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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pKM126 Resource Report Resource Website |
RRID:Addgene_105134 | Upstream & Downstream pyrE deletion region | C. difficile | Chloramphenicol | PMID:29116155 | Backbone Size:6324; Vector Backbone:pJS116; Vector Types:E. coli - C. difficile shuttle vector; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:00:48 | 0 | ||
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pJK02 Resource Report Resource Website 1+ mentions |
RRID:Addgene_105133 | Upstream & Downstream pyrE deletion region | C. difficile | Chloramphenicol | PMID:29116155 | Backbone Size:6297; Vector Backbone:pMTL84151; Vector Types:E. coli - C. difficile shuttle vector; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:00:48 | 1 | ||
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pAF257 Resource Report Resource Website 1+ mentions |
RRID:Addgene_105497 | SmBiT/HupA-LgBiT | C. difficile / synthetic | Chloramphenicol | PMID:30633871 | Please visit https://www.biorxiv.org/content/early/2018/09/27/426809 for bioRxiv preprint. | Backbone Marker:R.P. Fagan, University of Sheffield; Vector Backbone:pRPF185; Vector Types:ATc-dependent expression in C. difficile; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:00:56 | 1 | |
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pAF256 Resource Report Resource Website 1+ mentions |
RRID:Addgene_105496 | HupA-SmBiT/LgBiT | C. difficile / synthetic | Chloramphenicol | PMID:30633871 | Please visit https://www.biorxiv.org/content/early/2018/09/27/426809 for bioRxiv preprint. | Backbone Marker:R.P. Fagan, University of Sheffield; Vector Backbone:pRPF185; Vector Types:ATc-dependent expression in C. difficile; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:00:52 | 1 | |
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pYL322d1/N-eGFP Resource Report Resource Website 1+ mentions |
RRID:Addgene_183151 | Chloramphenicol | PMID:35179286 | Vector Backbone:pYL322d1; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:11:34 | 1 | ||||
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pSPK1 Resource Report Resource Website |
RRID:Addgene_18876 | polyphosphate kinase | E. coli | Chloramphenicol | PMID:9143103 | Backbone Size:0; Vector Backbone:pMMB206; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:11:40 | 0 | ||
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pDESTsmg Resource Report Resource Website |
RRID:Addgene_18878 | Chloramphenicol | PMID:17949993 | This is a Gateway destination vector. It requires a ccdB survival strain of E. coli for propagation of the plasmid, but once a gene of interest has been cloned in (via the LR reaction) it will grow in standard E. coli strains (eg. DH5alpha, BL21). This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning using E. coli. | Backbone Size:6573; Vector Backbone:pYUB1049; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:11:40 | 0 | |||
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pAK02 Resource Report Resource Website |
RRID:Addgene_19001 | arabinose transporter araE under control of Ptac | E. coli | Chloramphenicol | PMID:11092865 | Backbone Size:0; Vector Backbone:pMMB207; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:11:41 | 0 | ||
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pAC-PHYT Resource Report Resource Website |
RRID:Addgene_19002 | crtE, crtB | E. herbicola | Chloramphenicol | PMID:11676567 | Backbone Marker:Cunningham et al., 1994; Backbone Size:0; Vector Backbone:pAC-LYC; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:11:41 | 0 | ||
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pBTK102 Resource Report Resource Website |
RRID:Addgene_110574 | T7 promoter with RBS | Synthetic | Chloramphenicol | PMID:29608282 | Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:01:41 | 0 | ||
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pBTK107 Resource Report Resource Website |
RRID:Addgene_110575 | CP25 Promoter with RBS | Synthetic | Chloramphenicol | PMID:29608282 | Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:01:41 | 0 | ||
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pBTK113 Resource Report Resource Website |
RRID:Addgene_110578 | CP32 Promoter with RBS | Synthetic | Chloramphenicol | PMID:29608282 | Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:01:41 | 0 | ||
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pBTK112 Resource Report Resource Website |
RRID:Addgene_110577 | CP12b Promoter with RBS | Synthetic | Chloramphenicol | PMID:29608282 | Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:01:41 | 0 | ||
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T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB Resource Report Resource Website |
RRID:Addgene_110547 | dCas9 | Synthetic | Chloramphenicol | pIM2432 | Backbone Marker:Novagen/Agilent; Backbone Size:2412; Vector Backbone:pACYC; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | D10A, H840A | 2026-08-15 01:01:40 | 0 | |
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pBTK300 Resource Report Resource Website |
RRID:Addgene_110592 | rpoC | Synthetic | Chloramphenicol | PMID:29608282 | Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:01:41 | 0 | ||
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pBTK103 Resource Report Resource Website |
RRID:Addgene_110582 | Lac promoter with lac operator and RBS | Synthetic | Chloramphenicol | PMID:29608282 | Vector Backbone:ColE1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:01:41 | 0 | ||
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pUCDM Resource Report Resource Website |
RRID:Addgene_110742 | Chloramphenicol | PMID:17117155 | Backbone Size:2979; Vector Backbone:pUCDM; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:01:43 | 0 | ||||
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pxCas9CR4 Resource Report Resource Website |
RRID:Addgene_111656 | TetR and xCas9 | Synthetic | Chloramphenicol | Vector Backbone:p15A origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol | xCas9 mutations | 2026-08-15 01:01:53 | 0 | ||
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pACY.HR4_T7_term_HRR Resource Report Resource Website |
RRID:Addgene_111986 | Chloramphenicol | PMID:29724956 | Backbone Size:2787; Vector Backbone:pACYCDuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:01:56 | 0 | ||||
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pKOV Resource Report Resource Website 10+ mentions |
RRID:Addgene_25769 | Chloramphenicol | PMID:9335267 | pKOV is identical to the pKO3 vector described in J. Bacteriology 179: 6228-6237, except for the addition of a 3kb stuffer sequence in the multiple cloning site. This stuffer permits (i) directional cloning using NotI and BamHI and (ii) clean separation of doubly cut vector from singly cut contaminants when using this pair of enzymes. The pKOV cloning site is: 5' - SmaI - NotI - SmaI- stuffer - BamHI - SalI - 3'. BamHI and SalI are not used together, nor is SmaI used with NotI. BglII & BclI cut ends are compatible with BamHI. PmeI & SwaI are compatible with SmaI. NOTE: pKOV has a temperature sensitive pSC101 replication origin. To recover the plasmid, strains harboring the plasmid must be grown at 30 deg C under chloramphenicol selection. Gene replacement: Mutant alleles cloned into the pKOV gene replacement vector are electroporated into recombination proficient strains (eg. EMG2) and allowed to recover for 1 h at 30 deg C. The cells are plated on prewarmed chloramphenicol/LB plates and incubated at 42 deg C. To measure the integration frequency, the electroporated cells are also plated on chloramphenicol/LB plates at 30 deg C. From the 42 deg C plate, 1-5 colonies are picked into 1 ml of LB broth, serially diluted, and immediately plated at 30°ree;C on either 5% w/v sucrose or 5% sucrose+antibiotic plates. The 5% sucrose plates are replica plated to chloramphenicol plates at 30 deg C to test for loss of the replacement vector (cms). The gene replacement is confirmed by either PCR using primers flanking the targeted open reading frame or by genomic Southern's. Note from depositor: If sucrose plates do not select correctly, pKOV should be used in liquid selection on a plate reader with a LB + Cm control growth and a LB + Cm + sucrose growth. pKOV can sometimes still allow survival in the presence of sucrose, but significantly decreases fitness, so tracking the kinetic growth can be helpful. The sacB gene loses its efficacy,, sacB is toxic in E. coli even in the absence of sucrose. | Backbone Marker:N/A; Backbone Size:8673; Vector Backbone:pMAK700, pMAK705, pBS-TS; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol | 2026-08-15 01:12:47 | 10 |
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