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Species: Caenorhabditis elegans
Genetic Insert: snf-5
Vector Backbone Description: Backbone Size:5052; Vector Backbone:pXOOM; Vector Types:Mammalian Expression, Xenopus Oocyte Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23580723
Proper citation: RRID:Addgene_48697 Copy
Species: Synthetic
Genetic Insert: AmCyan
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:4000; Vector Backbone:pmR-mCherry; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: the nAmCyan and the mCherry genes are separated by a P2A sequence that results in 2 separate proteins. It also includes BsmBI restriction sites flanking the nAmCyan gene which result in BsiWI and BssHII overhangs for cloning in-frame with P2A-mCherry.
Proper citation: RRID:Addgene_48686 Copy
Species: Homo sapiens
Genetic Insert: KMT2B homology arms
Vector Backbone Description: Backbone Marker:Eric Mendenhall & Richard M. Myers (Addgene plasmid # 63934); Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_101070 Copy
Species: Homo sapiens
Genetic Insert: RERE homology arms
Vector Backbone Description: Backbone Marker:Eric Mendenhall & Richard M. Myers (Addgene plasmid # 63934); Vector Backbone:pFETCh_Donor; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26355004
Comments: Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
Proper citation: RRID:Addgene_101069 Copy
Species: Homo sapiens
Genetic Insert: Non-muscle myosin IIA heavy chain
Vector Backbone Description: Backbone Marker:clonetech; Vector Backbone:pEGFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28053086
Proper citation: RRID:Addgene_101040 Copy
Species: Homo sapiens
Genetic Insert: EED
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: EED:APC049-D10:C27444. SGC or PDB link: http://www.thesgc.org/structures/5TTW/
Proper citation: RRID:Addgene_101254 Copy
Species: Homo sapiens
Genetic Insert: Fgß peptide, ddFLN4 fingerprint
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29599244
Proper citation: RRID:Addgene_101239 Copy
Species: Homo sapiens
Genetic Insert: FFSARG peptide, ddFLN4 fingerprint
Vector Backbone Description: Backbone Marker:EMD Biosciences; Backbone Size:5216; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29599244
Proper citation: RRID:Addgene_101240 Copy
Species: Mus musculus
Genetic Insert: Rock2
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101296 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28329679
Comments: The TS module with mTFP1-F40-venusA206K was adapted from Vinculin TS (a kind gift from Martin Schwartz [Grashoff et al., 2010]). The TSmod was cloned in to pEGFP-N1 vector at Xho1 and Not1 restriction sites. For aE-Cat-TS, the mouse N-terminal (1_697 aa) of alpha-catenin was inserted before mTFP1 by in- fusion cloning (Takara Bioscience, Clontech) at the Xho1 restriction site
Proper citation: RRID:Addgene_101298 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101292 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101293 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Proper citation: RRID:Addgene_101294 Copy
Species: Mus musculus
Genetic Insert: alpha-Catenin
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28329679
Comments: The C-terminal fragment (698-906 aa) of alpha-catenin was cloned after VenusA206K at the Not1 restriction site
Proper citation: RRID:Addgene_101299 Copy
Species: Homo sapiens
Genetic Insert: EPHA3
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26094); Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: N terminal tag: MGSSHHHHHHSSGLVPRGS. SGC Clone Sample ID: EPHA3:SDC033-F08:C12121. SGC or PDB link: http://www.rcsb.org/pdb/explore/explore.do?structureId=2QO2
Proper citation: RRID:Addgene_101271 Copy
Species: Mus musculus
Genetic Insert: E-Cadherin-GFP-N-WASP
Vector Backbone Description: Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24413434
Comments: was generated by recombinining mEcad-GFP, a linker5"-GGGTCTACATCTGGATCTGGGAGGCCGGGTTCTGGTGAGGGTTCT3 and N-WASPdeltaVCA into NOT1/Age Iin gfp-N1 vector
Proper citation: RRID:Addgene_101283 Copy
Species: Mus musculus
Genetic Insert: Rock1
Vector Backbone Description: Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:26368311
Comments: The GFP-ROCK1 construct was generated by PCR-amplifying mouse ROCK1 using FLAG-tag mouse ROCK1 (a kind gift from M. Samuel, Centre for Cancer Biology, University of South Australia, Australia) as a template and cloned into pEGFP-C1 (Clontech) using SacI and KpnI restriction sites.
Proper citation: RRID:Addgene_101289 Copy
Species: Homo sapiens
Genetic Insert: RPUSD4
Vector Backbone Description: Backbone Marker:SGC (Addgene Plasmid #26096); Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: RPUSD4:JMC111-H03:C235563. SGC or PDB link: http://www.thesgc.org/structures/5UBA/
Proper citation: RRID:Addgene_101256 Copy
Species: Homo sapiens
Genetic Insert: BSX
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257861
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101410 Copy
Species: Homo sapiens
Genetic Insert: HES3
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pDONR221; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33257861
Comments: Harvard generated by gene synthesis
Proper citation: RRID:Addgene_101411 Copy
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