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Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184296 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184297 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184312 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184313 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184323 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184298 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184307 Copy
Species: Drosophila melanogaster
Genetic Insert: Act5C
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3328; Vector Backbone:pPICZ B; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin)
Defining Citation: PMID:35332323
Comments: Insert was expressed in fusion with beta-thymosin4. Can be expressed in GS115.
Proper citation: RRID:Addgene_184311 Copy
Species: Drosophila melanogaster
Genetic Insert: DmGIGYF_1-640
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31114929
Comments: Note that the V5-His tag is not part of the EGFP-DmGIGYF open reading frame. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148919 Copy
Species: Drosophila melanogaster
Genetic Insert: CBP1-287
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:4900; Vector Backbone:pGEX-2T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24550119
Proper citation: RRID:Addgene_182839 Copy
Species: Drosophila melanogaster
Genetic Insert: U6.3-gRNAs[sxl, bTub]
Vector Backbone Description: Vector Backbone:pBac; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_183135 Copy
Species: Drosophila melanogaster
Genetic Insert: U6.3-gRNAs[dsx]
Vector Backbone Description: Vector Backbone:pBac; Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_183133 Copy
Species: Drosophila melanogaster
Genetic Insert: CIRL
Vector Backbone Description: Vector Backbone:pTL412; Vector Types:transformation vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25937282
Proper citation: RRID:Addgene_69882 Copy
Species: Drosophila melanogaster
Genetic Insert: CIRL
Vector Backbone Description: Backbone Marker:Huang et al., 2009, PMID 19429710; Vector Backbone:pGE-attB-GMR; Vector Types:phiC31-integration vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25937282
Proper citation: RRID:Addgene_69881 Copy
Species: Drosophila melanogaster
Genetic Insert: DmGe1_1220-1354
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18755833
Comments: ORF extracted from NCBI: NM_135642.2. Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146420 Copy
Species: Drosophila melanogaster
Genetic Insert: U6:3-gRNA
Vector Backbone Description: Backbone Marker:Fillip Port; Vector Backbone:pCFD3-dU6-3gRNA (Addgene 49410); Vector Types:Insect Expression, CRISPR; Bacterial Resistance:Ampicillin
Comments: When describing the use of this plasmid in publications, please state that it is derived from pCFD3 and cite:
Port et al. Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):E2967-76. doi: 10.1073/pnas.1405500111
In pCFD3.1, the BbsI sites have been mutated in mini-white, which allows use of the remaining BbsI sites for gRNA cloning (using the protocol for pCFD3 (https://www.crisprflydesign.org/plasmids/)).
Proper citation: RRID:Addgene_123366 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Rae1
Vector Backbone Description: Backbone Size:2563; Vector Backbone:pDONR221; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28554770
Proper citation: RRID:Addgene_124240 Copy
Species: Drosophila melanogaster
Genetic Insert: Drosophila Actin 5C
Vector Backbone Description: Backbone Size:2553; Vector Backbone:pDONR221; Vector Types:Insect Expression; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_124242 Copy
Species: Drosophila melanogaster
Genetic Insert: wg gRNA
Vector Backbone Description: Backbone Size:7473; Vector Backbone:attB; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34078888
Proper citation: RRID:Addgene_125006 Copy
Species: Drosophila melanogaster
Genetic Insert: 2.4kb mhc enhancer element
Vector Backbone Description: Backbone Marker:Tudor A. Fulga; Backbone Size:11291; Vector Backbone:pJC-20xUAS-TALE3-VP64-P10; Vector Types:Insect Expression, TALEN; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29162808
Proper citation: RRID:Addgene_104611 Copy
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