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Species: Synthetic
Genetic Insert: Actb MMEJ donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28524166
Comments: gRNA sequence: agtccgcctagaagcacttg
Proper citation: RRID:Addgene_97315 Copy
Species: Synthetic
Genetic Insert: Actb NHEJ donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28524166
Comments: gRNA sequence: agtccgcctagaagcacttg
Proper citation: RRID:Addgene_97316 Copy
Species: Synthetic
Genetic Insert: Cdx2 HMEJ donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28524166
Comments: gRNA sequence: agaccacgggaggggtcact
Proper citation: RRID:Addgene_97319 Copy
Species: Synthetic
Genetic Insert: Nanog HMEJ donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28524166
Comments: gRNA sequence: cgtaagtctcatatttcacc
Proper citation: RRID:Addgene_97321 Copy
Species: Synthetic
Genetic Insert: Sox2 HMEJ donor
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, Mouse Targeting, AAV; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28524166
Comments: gRNA sequence: tgcccctgtcgcacatgtga
Proper citation: RRID:Addgene_97322 Copy
Species: E. coli
Genetic Insert: AraC/PBAD
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4559; Vector Backbone:pSAM_Ec; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:28614382
Comments: Mariner himar1C9 transposase driven by an arabinose promoter on an R6K gamma suicide plasmid. The transposon allows identification of fitness factors by Illumina sequencing. This transposon will randomly insert a kanamycin resistance cassette into genomic TA sites. Ampicillin resistance is contained on the plasmid backbone.
For further background, see:
Goodman, Andrew L., Meng Wu, and Jeffrey I. Gordon. “Identifying Microbial Fitness Determinants by Insertion Sequencing (INSeq) Using Genome-Wide Transposon Mutant Libraries.” Nature Protocols 6.12 (2011): 1969–1980. PMC. Web. 11 Apr. 2017.
Wiles, Travis J. et al. “Combining Quantitative Genetic Footprinting and Trait Enrichment Analysis to Identify Fitness Determinants of a Bacterial Pathogen.” Ed. Danielle A. Garsin. PLoS Genetics 9.8 (2013): e1003716. PMC. Web. 11 Apr. 2017.
Proper citation: RRID:Addgene_91569 Copy
Species: Other
Genetic Insert: OsTIR1-F74A
Vector Backbone Description: Vector Backbone:pDB4697; Vector Types:Other; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:34849776
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.19.452993v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171127 Copy
Species: S. pombe (fission yeast)
Genetic Insert: ura4
Vector Backbone Description: Backbone Marker:TransGen Biotech; Vector Backbone:pEASY-blunt; Vector Types:Other; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:34849776
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.07.19.452993v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_171125 Copy
Species: Synthetic
Genetic Insert: Internal Control sequence
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3929; Vector Backbone:pCR2.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This plasmid was created in the FDA ORA lab.
Proper citation: RRID:Addgene_83959 Copy
Species: Bacteria
Genetic Insert: miniTN7
Vector Backbone Description: Backbone Marker:NEB; Backbone Size:4163; Vector Backbone:pTrc99A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:8384534
Proper citation: RRID:Addgene_8487 Copy
Species: Homo sapiens
Genetic Insert: Homo sapiens hemoglobin subunit beta (HBB), Aberrant cDNA fragment (Exon1/19nt mispliced Intron1 + Exon2)
Vector Backbone Description: Backbone Marker:Invitrogen (Life Technologies); Backbone Size:3930; Vector Backbone:pCR2.1-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:32933098
Proper citation: RRID:Addgene_87848 Copy
Species: Synthetic
Genetic Insert: CAG-GFP
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pCR-TOPO; Vector Types:Mammalian Expression, donor vector (human); Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26707206
Comments: Constructed from Addgene plasmid #22075
Proper citation: RRID:Addgene_80491 Copy
Species: Synthetic
Genetic Insert: sfGFP-piggyBac-DsRed
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR™4-TOPO; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_80811 Copy
Species: Synthetic
Genetic Insert: 2xHA-piggyBac-DsRed
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3956; Vector Backbone:pCR™4-TOPO; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_80822 Copy
Species: Homo sapiens
Genetic Insert: AAVS1-eTLR
Vector Backbone Description: Backbone Size:3948; Vector Backbone:pBlueScript; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:38302659
Proper citation: RRID:Addgene_214737 Copy
Species: Synthetic
Genetic Insert: Recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
Vector Backbone Description: Backbone Size:1886; Vector Backbone:pYTK095; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37786689
Comments: Please visit https://doi.org/10.1101/2023.09.19.558440 for bioRxiv preprint.
Proper citation: RRID:Addgene_209544 Copy
Species: Synthetic
Genetic Insert: mVenus-FLAG-Kan
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Proper citation: RRID:Addgene_211830 Copy
Species: Mus musculus
Genetic Insert: Fzd6
Vector Backbone Description: Vector Backbone:pCR2.1; Vector Types:cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37622728
Proper citation: RRID:Addgene_208363 Copy
Species: Mus musculus
Genetic Insert: Fzd6
Vector Backbone Description: Vector Backbone:pCR2.1; Vector Types:cloning vector; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:37622728
Proper citation: RRID:Addgene_208364 Copy
Species: Homo sapiens
Genetic Insert: trPAG
Vector Backbone Description: Backbone Marker:Ilya Levental lab; Vector Backbone:trLAT-mRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:36997644
Proper citation: RRID:Addgene_203752 Copy
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