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Species: E. coli
Genetic Insert: MlaFEDCB
Vector Backbone Description: Vector Backbone:pBAD-His; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33236984
Proper citation: RRID:Addgene_164603 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pAN1818; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33784029
Comments: Derived from the pAN1818 plasmid from the Chris Voigt lab.
Proper citation: RRID:Addgene_164830 Copy
Species: E. coli
Genetic Insert: LacI
Vector Backbone Description: Vector Backbone:pAN1818; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:33784029
Proper citation: RRID:Addgene_164831 Copy
Species: E. coli
Genetic Insert: ptet_ompC_gfp
Vector Backbone Description: Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22618873
Proper citation: RRID:Addgene_37757 Copy
Species: E. coli
Genetic Insert: pLar_rpoS_gfp
Vector Backbone Description: Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22618873
Proper citation: RRID:Addgene_37758 Copy
Species: E. coli
Genetic Insert: ptet_ompC_gfp
Vector Backbone Description: Backbone Size:2000; Vector Backbone:ColE (from pZ system) + AmpR; Vector Types:Synthetic Biology, E. coli; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22618873
Proper citation: RRID:Addgene_37754 Copy
Species: E. coli
Genetic Insert: DsbC
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22197595
Proper citation: RRID:Addgene_38152 Copy
Species: E. coli
Genetic Insert: birA
Vector Backbone Description: Backbone Marker:Matthew Bogyo laboratory; Backbone Size:6126; Vector Backbone:Addgene Plasmid #38251 (pET22b-CPDSalI); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19956581
Comments: Encodes untagged biotin ligase gene (birA) from E. coli fused to Vibrio cholerae MARTX toxin cysteine protease domain (CPD) to be used to purify biotin ligase.
Proper citation: RRID:Addgene_38258 Copy
Species: E. coli
Genetic Insert: Sigma32 I54N
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25051296
Proper citation: RRID:Addgene_59982 Copy
Species: E. coli
Genetic Insert: tnpA from pBam1
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:6706; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25079858
Proper citation: RRID:Addgene_60240 Copy
Species: E. Coli
Genetic Insert: SspB nano
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:5979; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Comments: The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS
Proper citation: RRID:Addgene_60409 Copy
Species: E. Coli
Genetic Insert: SspB micro
Vector Backbone Description: Backbone Marker:Qiagen; Backbone Size:5979; Vector Backbone:pQE-80L; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25535392
Comments: The SspB used in this construct contains two amino acid mutations that disrupt the natural dimerization of SspB. These mutations are Y11K and A15E (residue numbering from pdb code 1ou9). Here is the sequence around the mutated residues with the sites of mutation in parentheses: SSPKRP(K)LLR(E)YYDWLVDNS
Proper citation: RRID:Addgene_60410 Copy
Species: E. Coli
Genetic Insert: GalS-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60753 Copy
Species: E. Coli
Genetic Insert: FruR-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60752 Copy
Species: E. Coli
Genetic Insert: RbsR-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60751 Copy
Species: E. Coli
Genetic Insert: TreR-T
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4040; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60754 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Comments: This plasmid contains a Y191CL mutation in the MET15 gene that does not impact selection or plasmid function.
Proper citation: RRID:Addgene_60933 Copy
Species: E. coli
Genetic Insert: Cre
Vector Backbone Description: Vector Backbone:pSH47; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25688547
Proper citation: RRID:Addgene_60931 Copy
Species: E. coli
Genetic Insert: msd(wt)_dRT
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZE32; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61454 Copy
Species: E. coli
Genetic Insert: SCRIBE_galK(OFF)
Vector Backbone Description: Backbone Marker:Expressys; Vector Backbone:pZA11; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25395541
Proper citation: RRID:Addgene_61455 Copy
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