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Vector Backbone Description: Backbone Size:3691; Vector Backbone:pBTBXh-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20148414
Comments: There is an extra G in the middle of the alignment between Addgene's quality control sequence and the author's sequence. This discrepancy is in a non-coding region and does affect function.
Proper citation: RRID:Addgene_26079 Copy
Vector Backbone Description: Backbone Size:4922; Vector Backbone:pBMTBX-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:20148414
Proper citation: RRID:Addgene_26074 Copy
Species: Aequorea victoria
Genetic Insert: destabilized GFP
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLuxI-tet8 + pRKM-102; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:15096621
Comments: A novel hybrid promoter luxPRcI-OR1 (wild-type luxPR promoter with a CI OR1 operator site inserted at the +1 transcription start) drives expression of GFP(LVA). The promoter was constructed by encoding OR1 on PCR primers and inserting it after luxPR
Proper citation: RRID:Addgene_26580 Copy
Species: Homo sapiens
Genetic Insert: p21 promoter deletion mutant
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5600; Vector Backbone:pBlueScript II KS(+); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:8988173
Proper citation: RRID:Addgene_21728 Copy
Species: Homo sapiens
Genetic Insert: p21 promoter deletion mutant
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5600; Vector Backbone:pBlueScript II KS(+); Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:8988173
Proper citation: RRID:Addgene_21730 Copy
Species: Synthetic
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Clonetech; Backbone Size:2070; Vector Backbone:pPROLar.A122; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19915540
Comments: MG1655 for expression
Proper citation: RRID:Addgene_34617 Copy
Species: Sperm Whale: Physeter macrocephalus
Genetic Insert: Myoglobin-127TAG
Vector Backbone Description: Vector Backbone:pSepT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21868676
Comments: To prevent possible enzymatic dephosphorylation of O-phospho-L-serine (Sep) in vivo, the gene encoding phosphoserine phosphatase (serB), which is catalyzing the last step in serine biosynthesis, was deleted from Escherichia coli strains Top10 (Top10∆serB - Addgene #34928) and BL21 (BL21∆serB - Addgene #34929). These strains are required hosts when using this plasmid.
Proper citation: RRID:Addgene_34625 Copy
Genetic Insert: SB100X transposase
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19412179
Comments: The Sleeping Beauty transposon system consists of two components: the transposon vector and the transposase vector. The transposon vector (pT4/HB, Addgene #108352) contains binding sites flanking the GOI. And the transposase vector (pCMV(CAT)T7-SB100, Addgene #34879) encodes for the enzyme mediating excision and integration of the GOI.
Proper citation: RRID:Addgene_34879 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
Vector Backbone Description: Vector Backbone:pBbA5c; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21952217
Comments: Please note that the R364 in Mevalonate Kinase (MK) may be susceptible to mutations. Although the original plasmid encoded R364C, Addgene's stock contains R364S. This mutation is not expected to impact overall function, however the exact effects are unknown.
Proper citation: RRID:Addgene_35152 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3489; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000053; Origin: colE1
Proper citation: RRID:Addgene_35287 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4904; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000050; Origin: SC101
Proper citation: RRID:Addgene_35284 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3476; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000056; Origin: p15A
Proper citation: RRID:Addgene_35290 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3495; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000044; Origin: colE1
Proper citation: RRID:Addgene_35278 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3113; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000038; Origin: p15A
Proper citation: RRID:Addgene_35272 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3126; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000035; Origin: colE1
Proper citation: RRID:Addgene_35269 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:3789; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000074; Origin: p15A
Proper citation: RRID:Addgene_35308 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2600; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000092; Origin: p15A
Proper citation: RRID:Addgene_35326 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4850; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000086; Origin: SC101
Proper citation: RRID:Addgene_35320 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:4031; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000095; Origin: SC101
Proper citation: RRID:Addgene_35329 Copy
Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2619; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000265; Origin: BBR1
Proper citation: RRID:Addgene_35366 Copy
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