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Species: Homo sapiens
Genetic Insert: HPV58L1+L2
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17010405
Proper citation: RRID:Addgene_37324 Copy
Species: Homo sapiens
Genetic Insert: HPV31L1+L2
Vector Backbone Description: Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17603495
Proper citation: RRID:Addgene_37322 Copy
Species: Drosophila melanogaster
Genetic Insert: ovoD1 genomic DNA fragment
Vector Backbone Description: Backbone Size:7815; Vector Backbone:pCaSpeR 2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8306893
Comments: Perrimon Lab plasmid #25
Please see associated publication for cloning details
Proper citation: RRID:Addgene_37282 Copy
Species: Drosophila melanogaster
Genetic Insert: orthodenticle
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBlueScript; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:1979296
Comments: Perrimon Lab plasmid #46
Proper citation: RRID:Addgene_37285 Copy
Vector Backbone Description: Backbone Size:4168; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22916025
Comments: For more information on Grunwald TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#grunwald
Proper citation: RRID:Addgene_37275 Copy
Vector Backbone Description: Backbone Size:4039; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22916025
Comments: For more information on Grunwald TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#grunwald
Proper citation: RRID:Addgene_37276 Copy
Species: Mus musculus
Genetic Insert: Foxd3
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mouse Targeting, Insertion vector; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37272 Copy
Species: Drosophila melanogaster
Genetic Insert: SOCS36E enhancer fragment
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression, Luciferase, JAK/STAT reporter construct; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16055650
Comments: Perrimon Lab plasmid #446
A 441-bp genomic fragment in the enhancer of SOCS36E containing two potential STAT92E-binding sites was amplified by PCR, using five different sets of oligos: (1) CTGCAGGAACCACTCAGAGTGCCTGCGTGT (PstI), GAATTCATACAAAACTGTCTTAGGTGTTTA (EcoRI); (2) CTGCAGGAACCACTCAGAGTGCCTGCGTGT (PstI), CTGCAGATACAAAACTGTCTTAGGTGTTTA (PstI); (3) GAATTCGAACCACTCAGAGTGCCTGCGTGT (EcoRI), GAATTCATACAAAACTGTCTTAGGTGTTTA (EcoRI); (4) AGATCTGAACCACTCAGAGTGCCTGCGTGT (BglII), AGATCTATACAAAACTGTCTTAGGTGTTTA (BglII); (5) GCGGCCGCGAACCACTCAGAGTGCCTGCGTGT (NotI), GCGGCCGCATACAAAACTGTCTTAGGTGTTTA (NotI).
Each amplified genomic fragment containing different restriction enzyme sites was sequentially subcloned into pUAST. The genomic fragment amplified using the first set of oligos was subcloned into the PstI/EcoRI sites of pUAST to generate 2XSTAT92E. The genomic fragment amplified using the second set of oligos was subcloned into the PstI site of 2XSTAT92E to generate 4XSTAT92E. The genomic fragment amplified using the third set of oligos was subcloned into the EcoRI site of 4XSTAT92E to generate 6XSTAT92E. The genomic fragment amplified using the fourth set of oligos was subcloned into the BglII site of 6XSTAT92E to generate 8XSTAT92E.
Next, the hsp70 minimal promoter element was amplified from pUAST by PCR using oligos GCGGCCGCAGCGGAGACTCTAGCGAGCG (NotI) and CTCGAGAATTCCCTATTCAGAGTTCT (XhoI). This
hsp70 minimal promoter was subcloned into the NotI/XhoI sites of 8XSTAT92E to generate 8XSTAT92E–hsp70. Again, the genomic fragment amplified using the fifth set of oligos was subcloned into the NotI site of 8XSTAT92E–hsp70 vector to generate 10XSTAT92E–hsp70.
Finally, an XhoI/XbaI fragment containing the firefly luciferase gene from the pGL3–luciferase vector (Promega) was subcloned into the XhoI/XbaI sites of 10XSTAT92E–hsp70 to generate 10XSTAT92E–luciferase.
Proper citation: RRID:Addgene_37393 Copy
Species: Mus musculus
Genetic Insert: Foxd3
Vector Backbone Description: Vector Backbone:unknown; Vector Types:Mouse Targeting, Insertion vector for RMCE; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37274 Copy
Species: Drosophila melanogaster
Genetic Insert: ovoD1 genomic DNA fragment
Vector Backbone Description: Backbone Size:7815; Vector Backbone:pCaSpeR 2; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8306893
Comments: Perrimon Lab plasmid #23
Please see associated publication for cloning details
Proper citation: RRID:Addgene_37280 Copy
Species: Mus musculus
Genetic Insert: Foxd3
Vector Backbone Description: Backbone Size:3000; Vector Backbone:pPNT4; Vector Types:Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18367558
Proper citation: RRID:Addgene_37268 Copy
Species: Drosophila melanogaster
Genetic Insert: hopscotch
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7796812
Comments: Perrimon Lab plasmid #100
Proper citation: RRID:Addgene_37301 Copy
Species: Drosophila melanogaster
Genetic Insert: hopTum-1
Vector Backbone Description: Backbone Marker:Addgene plasmid# 37299; Vector Backbone:pCaShs-hopscotch; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7796812
Comments: Perrimon Lab plasmid #101
The pCaShs-Tum construct was generated by the replacement of a 220 bp SacII-BstEII fragment from pCaShs-hop (Addgene plasmid# 37299) with the same fragment of PCR-generated DNA from hopTum-1 hemizygous flies.
This mutation is a G to A transversion at nucleotide 1641 resulting in the substitution of a Glu for a Gly at amino acid 341.
Proper citation: RRID:Addgene_37303 Copy
Species: Drosophila melanogaster
Genetic Insert: hopTum-1
Vector Backbone Description: Backbone Size:8904; Vector Backbone:pUAST; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7796812
Comments: Perrimon Lab plasmid #102
The pUAS-Tum was made by the insertion of the NotI-XbaI fragment from pCaShs-Tum (Addgene plasmid# 37303) into pUAST.
Proper citation: RRID:Addgene_37304 Copy
Species: Mus musculus
Genetic Insert: Foxd3
Vector Backbone Description: Backbone Marker:Stratgene; Backbone Size:3000; Vector Backbone:pBS; Vector Types:Mouse Targeting; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12381664
Proper citation: RRID:Addgene_37266 Copy
Species: Mus musculus
Genetic Insert: Foxd3
Vector Backbone Description: Backbone Size:4095; Vector Backbone:pCS2; Vector Types:Mammalian Expression, Overexpression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17092955
Comments: Please note the insert is comprised of the 1.4kb ORF surrounded by UTRs.
Proper citation: RRID:Addgene_37267 Copy
Species: Homo sapiens
Genetic Insert: Dynactin 2
Vector Backbone Description: Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327364
Comments: Please note that Addgene's sequencing results identified single nucleotide mismatches at bp# 2273 & 2347 when compared to the full plasmid sequence provided by the depositing laboratory. The mismatch at bp#2273 causes A7P mutation in the DCTN2 seqeuence.
Proper citation: RRID:Addgene_37388 Copy
Species: Homo sapiens
Genetic Insert: dynein light chain TcTex-type 3
Vector Backbone Description: Vector Backbone:pBABE; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22327364
Proper citation: RRID:Addgene_37390 Copy
Species: Drosophila melanogaster
Genetic Insert: porcupine
Vector Backbone Description: Backbone Size:7855; Vector Backbone:pCaSpeR-4; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8985181
Comments: Perrimon Lab plasmid #123
The EcoRI-XbaI genomic DNA covering the porc locus was cloned in pCaspeR4 to generate a genomic porc rescue construct.
Proper citation: RRID:Addgene_37376 Copy
Species: Drosophila melanogaster
Genetic Insert: porcupine
Vector Backbone Description: Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8985181
Comments: Perrimon Lab plasmid #124
Proper citation: RRID:Addgene_37377 Copy
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