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Vector Backbone Description: Backbone Size:10406; Vector Backbone:pDN0520; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210482 Copy
Vector Backbone Description: Backbone Size:10711; Vector Backbone:pKB11; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210487 Copy
Vector Backbone Description: Backbone Size:9355; Vector Backbone:pDN0520; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210483 Copy
Vector Backbone Description: Backbone Size:9604; Vector Backbone:pKB12; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210488 Copy
Vector Backbone Description: Backbone Size:10720; Vector Backbone:pDN0509; Vector Types:Yeast Expression, Synthetic Biology, Destination vector for Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:37979156
Comments: Please visit https://doi.org/10.1101/2023.10.02.560513 for bioRxiv preprint.
Proper citation: RRID:Addgene_210485 Copy
Vector Backbone Description: Backbone Marker:Dominic Esposito (Addgene plasmid # 11518); Backbone Size:8695; Vector Backbone:pDest-527; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39036966
Comments: Useful bacterial expression vector for purifying challenging proteins.
SuperHis (14xHis) ensures very high affinity to Ni-IMAC column.
mOCR tag is very negatively charged. This promotes proper folding, prevents aggregation and enables purification via ion-exchange.
Refer to DelProposto et al 2008 (DOI: 10.1016/j.pep.2008.08.011) for information on mOCR tag fusions.
Proper citation: RRID:Addgene_206874 Copy
Vector Backbone Description: Backbone Size:10995; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: K207A mutation in EYFP will not affect plasmid function.
Proper citation: RRID:Addgene_201128 Copy
Vector Backbone Description: Backbone Size:10602; Vector Backbone:plex_307; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: K207A mutation in EYFP will not affect plasmid function.
Proper citation: RRID:Addgene_201131 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197793 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197788 Copy
Vector Backbone Description: Backbone Marker:Self-made; Vector Backbone:Unspecified; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:36896468
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.10.15.511792v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_197800 Copy
Genetic Insert: pUC/ori, bla
Vector Backbone Description: Backbone Marker:Gateway(R) (Invitrogen); Backbone Size:3012; Vector Backbone:pDONRP2R-P3; Vector Types:Bacterial Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21203517
Proper citation: RRID:Addgene_27367 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Cm Resistance, neutral site II integration platform for Synechococcus
Vector Backbone Description: Vector Backbone:1572; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10812624
Comments: NSII vector
cloning sites: NheI, XhoI, SmaI, XbaI, StuI, SalI, and EcoRV.
Chloramphenicol cassette from pUC1819 (Cm resistant) as a BamHI fragment into BglII site of pAM1572 such that it reads away from the MCS and a ScaI digest gives a 3.9kb band. NSII vector; unique cloning sites NheI, XhoI, SmaI, XbaI, StuI, SalI, EcoRV.
Proper citation: RRID:Addgene_40239 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: promoterless luxAB reporter vector
Vector Backbone Description: Vector Backbone:1573; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10812624
Comments: NSII vector
Lux Genes: luxAB
cloning sites: NheI, XhoI, SmaI, XbaI, StuI, and SalI.
luxAB as SalI-PvuII from pLAVI (pAM884) into EcoRV-SalI cut pAM1573. SalI, StuI, XbaI, SmaI, XhoI, NheI still unique. Glows in E. coli.
Proper citation: RRID:Addgene_40241 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5488; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Please note that the hygromycin cassette in this plasmid is non-functional. See provided protocol for high-throughput, ligation-independent cloning using this plasmid.
Proper citation: RRID:Addgene_40728 Copy
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pcDNA5/FRT/TO; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:20733051
Comments: Venus YFP-3xFlag was inserted via BstXI-XhoI sites (to create pcDNA5/FRT/TO-Venus-Flag, Addgene Plasmid# 40998).
The plasmid was then converted to a Gateway destination vector by inserting Gateway cassette C1, containing recombination sites attR1 and attR2, CmR, and ccdB, into the Afl II site (blunt ended).
This plasmid can be used to place genes into the pcDNA5/FRT/TO-Venus-Flag construct by Gateway reaction, to make tetracycline-inducible stable cell lines.
For subcloning, the following restriction sites are present:
Between the CMV promoter and the Gateway cassette: PmeI (non-unique)
Between the Gateway cassette and Venus: HindIII, Asp718I, KpnI, BamHI (non-unique, also in GW casette), and BstXI
Between Venus and BGHpA: XhoI, Eco0109I, ApaI, and PmeI (non-unique)
Proper citation: RRID:Addgene_40999 Copy
Genetic Insert: TALE Nuclease, NG for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32190 Copy
Genetic Insert: TALE Transcriptional Activator Backbone, NI for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32187 Copy
Genetic Insert: TALE Transcriptional Activator Backbone, NN for 0.5 repeat
Vector Backbone Description: Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22222791
Proper citation: RRID:Addgene_32185 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJFRC7; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21931740
Comments: Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.
Proper citation: RRID:Addgene_32318 Copy
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