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Species: Danio rerio
Genetic Insert: kcnh5a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255307 Copy
Species: Danio rerio
Genetic Insert: kcnh2a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255302 Copy
Species: Danio rerio
Genetic Insert: kcnh7a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255311 Copy
Species: Danio rerio
Genetic Insert: kcnh6b
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255310 Copy
Species: Danio rerio
Genetic Insert: kcnh8
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2606; Vector Backbone:pENTR-D-sp6; Vector Types:Mammalian Expression, Gateway Cloning, In vitro transcription for riboprobes; Bacterial Resistance:Kanamycin
Defining Citation: PMID:42496271
Proper citation: RRID:Addgene_255313 Copy
Species: Danio rerio
Genetic Insert: zSynC
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3961; Vector Backbone:PCRII-TOPO; Vector Types:in-situ; Bacterial Resistance:Ampicillin
Comments: zSynC in situ probe.
For antisense, cut with BamHI and transcribe with T7.
Proper citation: RRID:Addgene_20123 Copy
Species: Danio rerio
Genetic Insert: Wnt5b
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17185322
Comments: Zebrafish wnt5 (pipetail) cDNA for antisense probe. Full-length ORF plus some (exact sequence not known, likely genbank NM_130937) 5' and 3' UTRs, 1.75kb total insert. Cloned into EcoRI site of pBluescript (there is also an internal EcoRI!). From Phil Inghams' lab, cloned by P. Blader.
Used to be called wnt5 and consider by many people to be wnt5a. however, after Gilbert cloned the real wnt5a, it is now known as wnt5b.
For antisense zWnt5b: cut w/ XbaI, transcribe w/ T3.
Proper citation: RRID:Addgene_21282 Copy
Species: Danio rerio
Genetic Insert: CaSR-EGFP-SV40
Vector Backbone Description: Backbone Size:2656; Vector Backbone:pENTR; Vector Types:zebrafish expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:36476852
Proper citation: RRID:Addgene_191503 Copy
Species: Danio rerio
Genetic Insert: 4xU6:sgRNA
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:39575683
Proper citation: RRID:Addgene_227778 Copy
Species: Danio rerio
Genetic Insert: Cas9-t2A-GFP
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39575683
Proper citation: RRID:Addgene_227773 Copy
Species: Danio rerio
Genetic Insert: loxP-dsR-loxP
Vector Backbone Description: Vector Backbone:Middle entry Cas9-t2A-GFP; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:39575683
Proper citation: RRID:Addgene_227774 Copy
Species: Danio rerio
Genetic Insert: Cas9-t2A-GFP
Vector Backbone Description: Vector Backbone:Tol2; Vector Types:CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:39575683
Proper citation: RRID:Addgene_226834 Copy
Species: Danio rerio
Genetic Insert: hsN-drAgo2
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221717 Copy
Species: Danio rerio
Genetic Insert: drAgo2D-A
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Proper citation: RRID:Addgene_221713 Copy
Species: Danio rerio
Genetic Insert: BleoR-miR-430
Vector Backbone Description: Vector Backbone:pCS2+; Vector Types:Fish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:29272705
Comments: This plasmid was used for IVT-template generation. Mutations may exist within the pre-existing CMV IE94 promoter.
Proper citation: RRID:Addgene_221726 Copy
Species: Danio rerio
Genetic Insert: mhc2dab
Vector Backbone Description: Vector Backbone:mTol2-mcherry; Vector Types:Zebrafish expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21406720
Comments: 3.8kb upstream of DAB
Proper citation: RRID:Addgene_68997 Copy
Species: Danio rerio
Genetic Insert: gsc2-sgRNA
Vector Backbone Description: Backbone Size:2147; Vector Backbone:pDR274; Vector Types:CRISPR, template for sgRNA synthesis; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38819436
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487414v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_184818 Copy
Species: Danio rerio
Genetic Insert: nts
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pCRII-TOPO; Vector Types:template for synthesis of nts probe; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38819436
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487414v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_184823 Copy
Species: Danio rerio
Genetic Insert: rln3a-HA
Vector Backbone Description: Backbone Size:7395; Vector Backbone:pPRISM; Vector Types:CRISPR, For integration of QF2 by homology-directed repair; Bacterial Resistance:Kanamycin
Defining Citation: PMID:38819436
Comments: Please visit https://www.biorxiv.org/content/10.1101/2022.04.07.487414v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_184820 Copy
Species: Danio rerio
Genetic Insert: CTNNA1
Vector Backbone Description: Vector Backbone:pGEX-6P-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:36539037
Proper citation: RRID:Addgene_191740 Copy
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