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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pEMB018 / LRRK2_BC_1520_2210 Resource Report Resource Website |
RRID:Addgene_40522 | LRRK2 | Homo sapiens | Ampicillin | Backbone Marker:Emerald; Vector Backbone:pEMB018; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | WT; aa1520_2210 | 2026-08-15 01:14:37 | 0 | ||
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pEMB018 / LRRK2_BC_1520_2187 Resource Report Resource Website |
RRID:Addgene_40521 | LRRK2 | Homo sapiens | Ampicillin | Backbone Marker:Emerald; Vector Backbone:pEMB018; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | WT; aa1520_2187 | 2026-08-15 01:14:37 | 0 | ||
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pEMB018 / LRRK2_BC_1309_2350 Resource Report Resource Website |
RRID:Addgene_40529 | LRRK2 | Homo sapiens | Ampicillin | Backbone Marker:Emerald; Vector Backbone:pEMB018; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | WT; aa1309_2350 | 2026-08-15 01:14:37 | 0 | ||
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pGEX4T-1_OCT4_T234A Resource Report Resource Website |
RRID:Addgene_40637 | POU5F1 | Homo sapiens | Ampicillin | PMID:22474382 | Backbone Marker:GE-healthcare; Backbone Size:6237; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | T234A | 2026-08-15 01:14:38 | 0 | |
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pGEX4T-1_OCT4_T234E Resource Report Resource Website |
RRID:Addgene_40636 | POU5F1 | Homo sapiens | Ampicillin | PMID:22474382 | Backbone Marker:GE-healthcare; Backbone Size:6237; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | T234E | 2026-08-15 01:14:38 | 0 | |
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pGEX4T-1_OCT4_T234A_S235A Resource Report Resource Website |
RRID:Addgene_40634 | POU5F1 | Homo sapiens | Ampicillin | PMID:22474382 | Backbone Marker:Invitrogen; Backbone Size:10712; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | T234A_S235A | 2026-08-15 01:14:38 | 0 | |
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pGEX4T-1_OCT4_S235A Resource Report Resource Website |
RRID:Addgene_40639 | POU5F1 | Homo sapiens | Ampicillin | PMID:22474382 | Backbone Marker:GE-healthcare; Backbone Size:6237; Vector Backbone:pGEX4T-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S235A | 2026-08-15 01:14:38 | 0 | |
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pCEP4_OCT4_T234A_S235A Resource Report Resource Website 1+ mentions |
RRID:Addgene_40630 | POU5F1_S234A_T235A | Homo sapiens | Ampicillin | PMID:22474382 | Backbone Marker:Invitrogen; Backbone Size:10712; Vector Backbone:pCEP4; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | S234A_T235A | 2026-08-15 01:14:38 | 1 | |
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hGFAP-Cre Resource Report Resource Website 1+ mentions |
RRID:Addgene_40591 | Cre recombinase | Homo sapiens | Ampicillin | PMID:11668683 | This plasmid encodes a transgene to express the Cre recombinase in astrocytes of transgenic mice. A DNA fragment encoding the Cre recombinase was inserted into an expression cassette containing the gfa2 promoter (Besnard et al., 1991), a 2.2-kb 5' flanking region from the human GFAP (hGFAP) gene. The nuclear targeting signal from the SV40 large T antigen was inserted at the amino terminal end of the coding region, because it reportedly increases the efficiency of DNA excision by the recombinase (Gu et al., 1993). A heterologous intron and polyadenylation signal were provided by sequences from the mouse protamine 1 gene at the 3' end. The hGFAP-cre transgene was constructed by excising the lacZ coding region from pgfa2-lac2 (Brenner et al., 1994) by BamHI digestion, and replacing it by blunt end ligation with a nuclear-targeted Cre coding sequence. The latter was obtained as a 1.1-kb SalI-MluI fragment from plasmid pTZ19RCreNLS, which was kindly provided by Drs. Raul Torres and Klaus Rajewsky. Both junctions were verified by DNA sequencing. | Backbone Marker:Michael Brenner (PMID: 7952266); Backbone Size:5500; Vector Backbone:pgfa2-lac2; Vector Types:Mammalian Expression, Mouse Targeting, Cre/Lox; Bacterial Resistance:Ampicillin | Construct contains a nuclear-targeted Cre recombinase followed by a heterologous mouse intron and polyadenylation signal, all under the control of the gfa2 promoter | 2026-08-15 01:14:37 | 8 |
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PLXNA4A Resource Report Resource Website |
RRID:Addgene_40748 | PLXNA4A | Homo sapiens | Kanamycin | http://www.thesgc.org/structures/4E74/ | Backbone Marker:SGC; Vector Backbone:pET28-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:38 | 0 | ||
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pXL010-Wnt dual (GFP-Fire) reporter Resource Report Resource Website 1+ mentions |
RRID:Addgene_40588 | Wnt reporter sequences | Homo sapiens | Ampicillin | PMID:22177620 | This lentiviral Wnt reporter has 7 repeats of Tcf/Lef binding sites, which was cloned by Xiaojun (Lance) Lian. Methods for Plasmid construction, lentiviral production and infection of hESCs: The 7 TCF/LEF binding site sequences were obtained by direct PCR of plasmid M50 (Addgene plasmid 12456) and the GreenFire (GF) sequences were obtained by direct PCR of the pGreenFire1-mCMV Plasmid (System Bioscience TR010PA-1). These two sequences were cloned into pSicoR PGK puro (Addgene plasmid 11586) digested by Xba I (NEB) and Xho I (NEB). The constructed Wnt reporter plasmid was named 7TGFP and verified by sequencing. This 7TGFP vector was cotransfected with the helper plasmids psPAX2 and pMD2.G (Addgene plasmids 12260 and 12259) into HEK-293TN cells (System Biosciences) for virus production. Virus-containing medium was collected at 48 and 72 hours after transfection and used for infection of hESCs in the presence of 6μg/mL polybrene (Sigma). Transduced cells were cultured in mTeSR1 on Matrigel for three days and then clonally isolated in mTeSR1 with 1 μg/mL puromycin. | Backbone Marker:addgene; Vector Backbone:pSicoR PGK Puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:37 | 3 | |
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pcDNA3-HA-TCF1 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40620 | TCF7 | Homo sapiens | Ampicillin | PMID:22949634 | Backbone Size:5400; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:38 | 4 | ||
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pCDH-F-PA1 Resource Report Resource Website |
RRID:Addgene_40618 | PA1 | Homo sapiens | Ampicillin | PMID:22949634 | Backbone Marker:System Biosciences (SBI); Backbone Size:8295; Vector Backbone:pCDH-EF1-MCS-IRES-Neo; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:37 | 0 | ||
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RPRD1B Resource Report Resource Website |
RRID:Addgene_40739 | RPRD1B | Homo sapiens | Ampicillin | http://www.thesgc.org/structures/4FLA/ | Backbone Marker:SGC; Vector Backbone:pET15-MHL; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:38 | 0 | ||
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L3MBTL3 Resource Report Resource Website |
RRID:Addgene_40738 | L3MBTL3 | Homo sapiens | Kanamycin | http://www.thesgc.org/structures/4FL6/ | Backbone Marker:SGC; Vector Backbone:pNIC-CH; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:38 | 0 | ||
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pEMB018 LRRK2_BC_1520_2148_K1906M Resource Report Resource Website |
RRID:Addgene_40567 | LRRK2 | Homo sapiens | Ampicillin | Backbone Marker:Emerald; Vector Backbone:pEMB108; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | aa1520_2148; K1906M | 2026-08-15 01:14:37 | 0 | ||
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pEMB52 LRRK2_BC_1520_2210_I2211ENLYFQGWSHPQFEK Resource Report Resource Website |
RRID:Addgene_40565 | LRRK2 | Homo sapiens | Ampicillin | Backbone Marker:Emerald; Vector Backbone:pEMB52; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | WT; aa1520_2210 | 2026-08-15 01:14:37 | 0 | ||
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pEMB12 LRRK2_BC_1847_2210_K1906M Resource Report Resource Website |
RRID:Addgene_40564 | LRRK2 | Homo sapiens | Ampicillin | Backbone Marker:Emerald; Vector Backbone:pEMB12; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | aa1847_2210; K1906M | 2026-08-15 01:14:37 | 0 | ||
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pEMB36(revcomp)/Dvl1_1_670 Resource Report Resource Website |
RRID:Addgene_40557 | DVL1 | Homo sapiens | Ampicillin | The insert has been codon optimized. | Backbone Marker:Emerald; Vector Backbone:pEMB36; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | WT; aa1_670 | 2026-08-15 01:14:37 | 0 | |
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pEMB12 / LRRK2_BC_1847_2210 Resource Report Resource Website |
RRID:Addgene_40553 | LRRK2 | Homo sapiens | Ampicillin | Backbone Marker:Emerald; Vector Backbone:pEMB12; Vector Types:Baculovirus; Bacterial Resistance:Ampicillin | WT; aa1847_2210 | 2026-08-15 01:14:37 | 0 |
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