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Species: Saccharomyces cerevisiae
Genetic Insert: sgf11
Vector Backbone Description: Backbone Marker:novagen; Backbone Size:3620; Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:20395473
Proper citation: RRID:Addgene_26960 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDC28
Vector Backbone Description: Backbone Size:4384; Vector Backbone:RS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21246629
Proper citation: RRID:Addgene_27331 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDC28
Vector Backbone Description: Backbone Size:4384; Vector Backbone:RS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21246629
Proper citation: RRID:Addgene_27330 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDH1
Vector Backbone Description: Backbone Size:4384; Vector Backbone:RS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21246629
Proper citation: RRID:Addgene_27328 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: single chain Cse4-Scm3-H4 with 2 Thrombin site
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pET42+b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21412236
Proper citation: RRID:Addgene_28250 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: single chain Cse4-H4 with Thrombin site
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pET42+b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21412236
Proper citation: RRID:Addgene_28251 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Fus3-as2
Vector Backbone Description: Backbone Size:4700; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079053
Comments: There are several sequence discrepancies between depositor's sequence and Addgene sequence in the 5' UTR. The 5'UTR in the plasmid is from a W303-derived strain, and the SGD used S288C strain for genome sequencing, so the mismatches could be SNPs. This plasmid is functional and these mismatches do not affect the ORF.
Proper citation: RRID:Addgene_21789 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Dig1-YFP
Vector Backbone Description: Backbone Size:3300; Vector Backbone:pACL7-YFP-A206K; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19079053
Comments: Construction was complicated; please refer to parent article's supplemental materials for details of construction.
Proper citation: RRID:Addgene_21788 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: REV1
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19528320
Comments: The REV1 ORF plus 1050 nucleotides (nt) of its upstream region that include the REV1 promoter and 500nt of the downstream region that include the REV1 transcription terminator was cloned in the vector YCplac111, which carries the LEU2 gene, the autonomous replicating sequence ARS1, and the centromeric CEN4 region.
A BglII restriction site was added by site-directed mutagenesis right before the stop codon of REV1. A 33-mer oligonucleotide duplex containing the HA-coding sequence and a BamHI and a BglII restriction site was inserted into the BglII restriction site, adding an HA tag in fusion with REV1, and regenerating a unique BglII restriction site. This step was repeated three times in order to get four HA tags in fusion with REV1.
Proper citation: RRID:Addgene_22287 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD5
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18757916
Comments: Mutation abolishes the ubiquitin ligase function
Proper citation: RRID:Addgene_22288 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: RAD5
Vector Backbone Description: Backbone Size:6112; Vector Backbone:YCplac111; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18757916
Proper citation: RRID:Addgene_22289 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23056 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23068 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23064 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III - Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23065 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9606197
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23066 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12039950
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23096 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12039950
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23097 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Tpa1
Vector Backbone Description: Backbone Size:5443; Vector Backbone:pET21a(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20040577
Comments: This construct has a mutation causing N565D in the aa sequence. The depositor does know the effect of this mutation. However, it is not part of the active site.
Proper citation: RRID:Addgene_23140 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yeast Histone H3-2 and Histone H4-2
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:4348; Vector Backbone:pRS414 delta Afl III- Sma I; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12620224
Comments: HHT2 is Yeast ID YNL031C
HHF2 is Yeast ID YNL030W
HHT2 is gene ID 855700
HHF2 is gene ID 855701
Proper citation: RRID:Addgene_23080 Copy
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