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Species: Danio rerio
Genetic Insert: sod1 guide RNA sequences
Vector Backbone Description: Backbone Marker:Qing Deng; Backbone Size:4000; Vector Backbone:p3E-U6a-U6c; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107603 Copy
Species: Danio rerio
Genetic Insert: uqcrc1 guide RNA sequences
Vector Backbone Description: Backbone Marker:Qing Deng; Backbone Size:4000; Vector Backbone:p3E-U6a-U6c; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107602 Copy
Species: Danio rerio
Genetic Insert: U6a promoter
Vector Backbone Description: Backbone Size:2651; Vector Backbone:p3E; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107593 Copy
Species: Danio rerio
Genetic Insert: cas9-NLS-P2A-mCherry
Vector Backbone Description: Backbone Size:2726; Vector Backbone:pME; Vector Types:CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107592 Copy
Species: Danio rerio
Genetic Insert: Lysozyme C promoter
Vector Backbone Description: Backbone Size:2693; Vector Backbone:p5E; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29590639
Comments: infusion cloning was used
Proper citation: RRID:Addgene_107591 Copy
Species: Danio rerio
Genetic Insert: U6a:2xMS2-sgRNA
Vector Backbone Description: Backbone Size:3565; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119076 Copy
Species: Danio rerio
Genetic Insert: ubb:MCP-p65-HSF1-2a-Cerulean
Vector Backbone Description: Backbone Size:3613; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119074 Copy
Species: Danio rerio
Genetic Insert: ubb:Com-KRAB-2a-Cerulean
Vector Backbone Description: Backbone Size:3613; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119075 Copy
Species: Danio rerio
Genetic Insert: U6a:scrambled3-sgRNA
Vector Backbone Description: Backbone Size:3577; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Contains scrambled spacer sequence ACTCGACGACGCACGTGTGC.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119072 Copy
Species: Danio rerio
Genetic Insert: U6a:scrambled4-sgRNA
Vector Backbone Description: Backbone Size:3577; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Contains scrambled spacer sequence ATCTATACTCTATACGAATA.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119073 Copy
Species: Danio rerio
Genetic Insert: pax3a_enh5-E1b:eGFP
Vector Backbone Description: Backbone Size:3858; Vector Backbone:Unknown; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: A. Emelyanov and S. Parinov. Mifepristone-inducible LexPR system to drive and control gene expression in transgenic zebrafish. Developmental Biology, 320(1):113– 121, 2008. ISSN 00121606. doi: 10.1016/j.ydbio.2008.04.042.
A. Emelyanov, Y. Gao, N. I. Naqvi, and S. Parinov. Trans-kingdom transposition of the maize Dissociation element. Genetics, 174(3):1095–1104, 2006. ISSN 00166731. doi: 10.1534/genetics.106.061184.
Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119059 Copy
Species: Danio rerio
Genetic Insert: pax3a_enh5-E1b:eGFP
Vector Backbone Description: Backbone Size:3671; Vector Backbone:pMT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:37367831
Comments: Please visit https://www.biorxiv.org/content/10.1101/450684v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_119061 Copy
Species: Danio rerio
Genetic Insert: zGrad
Vector Backbone Description: Vector Backbone:pCS2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:30735119
Proper citation: RRID:Addgene_119716 Copy
Species: Danio rerio
Genetic Insert: myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Vector Backbone Description: Backbone Size:3121; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32084358
Proper citation: RRID:Addgene_119865 Copy
Species: Danio rerio
Genetic Insert: myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Vector Backbone Description: Backbone Size:3121; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32084358
Proper citation: RRID:Addgene_119866 Copy
Species: Danio rerio
Genetic Insert: BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
Vector Backbone Description: Backbone Size:3017; Vector Backbone:pGEM-T; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32084358
Proper citation: RRID:Addgene_119864 Copy
Species: Danio rerio
Genetic Insert: dre-WT CDK2
Vector Backbone Description: Backbone Size:8500; Vector Backbone:tol2 insertion; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31451657
Proper citation: RRID:Addgene_121131 Copy
Species: Danio rerio
Genetic Insert: dre-cdk2 3'UTR
Vector Backbone Description: Backbone Size:6273; Vector Backbone:sicheck2; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31451657
Proper citation: RRID:Addgene_121123 Copy
Species: Danio rerio
Genetic Insert: IgZ
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3948; Vector Backbone:pCRII; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15685175
Comments: Secreted form of zeta. This clone was amplified using primers c2ch1A (5'-CACAGCACCAGTTGTGTTCAA) and c2endD (5'-TAATCTAACGGGTGAGCAACA).
Proper citation: RRID:Addgene_11302 Copy
Species: Danio rerio
Genetic Insert: he1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2640; Vector Backbone:pDONR P2R-P3; Vector Types:Zebrafish Danio expression; Bacterial Resistance:Kanamycin
Comments: Cloned from the construct provided by Jeff Mumm's Lab, Tol2 5xUAS-mCFP-p2A-NTRmut;HE:mCerulean
Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner."
BMC Biology 2012 10:93
https://doi.org/10.1186/1741-7007-10-93
Proper citation: RRID:Addgene_113880 Copy
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