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Species: Synthetic
Genetic Insert: RFP
Vector Backbone Description: Backbone Size:2137; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000267; Origin: colE1
Proper citation: RRID:Addgene_35364 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:3971; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000122; Origin: BBR1
Proper citation: RRID:Addgene_35356 Copy
Species: Synthetic
Genetic Insert: GFP
Vector Backbone Description: Backbone Size:4219; Vector Backbone:pBb; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21933410
Comments: JBEI Part ID: JPUB_000116; Origin: BBR1
Proper citation: RRID:Addgene_35350 Copy
Species: E. coli
Genetic Insert: lacIq
Vector Backbone Description: Backbone Size:4945; Vector Backbone:N/A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17565681
Comments: Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.
de Marco A.
Nat Protoc. 2007;2(10):2632-9.
Proper citation: RRID:Addgene_27390 Copy
Genetic Insert: qtag22
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4522; Vector Backbone:pTCS-2M1-icl2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21203517
Proper citation: RRID:Addgene_27335 Copy
Genetic Insert: qtag17
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:4522; Vector Backbone:pTCS-2M1-icl2; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21203517
Proper citation: RRID:Addgene_27333 Copy
Genetic Insert: qtag20
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:5740; Vector Backbone:pDE43-MCKq1; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21203517
Proper citation: RRID:Addgene_27334 Copy
Vector Backbone Description: Backbone Size:7484; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11483365
Comments: pTGV-Express contains PBAD and also includes the Shine-Dalgarno prokaryotic translation-initiation sequence for expression of heterologous genes in E. coli.
A single nucleotide deletion was detected in Addgene's quality control sequence compared to the depositing scientist's sequence at bp# 3277. The depositing scientist says this deletion has no impact on the function of the plasmid.
Proper citation: RRID:Addgene_28039 Copy
Vector Backbone Description: Backbone Size:7240; Vector Backbone:n/a; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:11483365
Comments: Note that Addgene's sequencing results found a 1 nucleotide mismatch at bp# 238 and 1 nucleotide deletions at bp# 2970 & 3016 when compared with the sequence provided by the depositing laboratory. These differences are not known to affect plasmid function.
Proper citation: RRID:Addgene_28033 Copy
Species: L. pneumophila
Genetic Insert: fabI
Vector Backbone Description: Backbone Size:9869; Vector Backbone:pXDC61; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19578436
Proper citation: RRID:Addgene_21842 Copy
Species: Mesoplasma florum
Genetic Insert: Lon
Vector Backbone Description: Backbone Marker:Beckwith Lab; Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:18852454
Comments: A detailed protocol for purification of mf-lon can be found in the associated paper.
Proper citation: RRID:Addgene_21867 Copy
Vector Backbone Description: Backbone Size:3586; Vector Backbone:pBTB-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16496398
Comments: Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.
Proper citation: RRID:Addgene_22819 Copy
Species: Synechocystis/E.coli fusion gene
Genetic Insert: pCph8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:2021; Vector Backbone:pPROTet; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16306980
Comments: This plasmid was also used in the 2009 Cell paper: "A synthetic genetic edge detection program." PMID:19563759
Proper citation: RRID:Addgene_22869 Copy
Vector Backbone Description: Backbone Size:4831; Vector Backbone:pBMTB-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16496398
Comments: Addgene's QC sequence shows a 4bp gap with the depositor's provided sequence. The lab does not believe this affects the plasmid activity.
Proper citation: RRID:Addgene_22826 Copy
Vector Backbone Description: Backbone Size:3649; Vector Backbone:pBMT-3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:16496398
Proper citation: RRID:Addgene_22838 Copy
Species: Mus musculus
Genetic Insert: DHFR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pBAD/HisA & pBThybrid; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19165721
Proper citation: RRID:Addgene_23011 Copy
Species: M.jannaschii
Genetic Insert: M.j. p-azidohenylalanine RS (2 copies +tRNA)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:12148987
Proper citation: RRID:Addgene_31186 Copy
Genetic Insert: ccdB and Cm resistance gene
Vector Backbone Description: Backbone Size:12617; Vector Backbone:pDD62; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21493687
Proper citation: RRID:Addgene_31224 Copy
Species: Synechocystis
Genetic Insert: pJT118
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1839; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21035461
Proper citation: RRID:Addgene_31395 Copy
Species: T7 phage
Genetic Insert: RNA polymerase
Vector Backbone Description: Backbone Size:5400; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:10610690
Comments: For expression: E. coli CV2
N823D mutation should not affect plasmid function.
Proper citation: RRID:Addgene_31491 Copy
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