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Species: Synthetic
Genetic Insert: pea APX W41F
Vector Backbone Description: Backbone Size:2226; Vector Backbone:pZE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24803433
Comments: This plasmid encodes the W41F mutant of pea ascorbate peroxidase (APX). The W41F mutation improves enzyme activity. APX W41F is a constitutive homodimer, unlike "APEX", so APX W41F should not be used as a fusion to proteins that are sensitive to dimeric tags.
This plasmid encodes APX W41F under a constitutive promoter, and the promoter is flanked by TetR operator sites. When the plasmid is introduced into cells constitutively expressing the TetR protein, such as the GM1655 Pro cells, then the TetR protein binds to the promoter and suppresses transcription. When a tetracycline drug, such as anhydrotetracycline, is added, the TetR protein is displaced from the promoter, allowing transcription of the APX W41F gene. When the plasmid is introduced into bacterial strains that do not encode a copy of the TetR repressor, expression of APX W41F is constitutive. Any bacterial strain can be used for plasmid propagation.
The pZE plasmid system was developed by Lutz and Bujard, and described in their paper entitled "Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements" (PMID: 9092630).
Proper citation: RRID:Addgene_58199 Copy
Species: Synthetic
Genetic Insert: U6.gRNAS1 cassette
Vector Backbone Description: Backbone Size:6930; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58252 Copy
Species: Synthetic
Genetic Insert: U6.gRNAGFP cassette
Vector Backbone Description: Backbone Size:7043; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58255 Copy
Species: Homo sapiens
Genetic Insert: ZO1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTomato-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58147 Copy
Species: Homo sapiens
Genetic Insert: Actin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58149 Copy
Species: Homo sapiens
Genetic Insert: Zyxin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18057027
Comments: . Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58148 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells.
Proper citation: RRID:Addgene_58158 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells. Excitation = 490 / 587; Emission = 511 / 610
Proper citation: RRID:Addgene_58157 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells.
Proper citation: RRID:Addgene_58159 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells. Excitation = 462 / 548; Emission = 492 / 561
Proper citation: RRID:Addgene_58152 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells. Excitation = 433 / 515; Emission = 475 / 528
Proper citation: RRID:Addgene_58151 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells. Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58156 Copy
Species: Homo sapiens
Genetic Insert: IL36RN
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:4900; Vector Backbone:pCMV6-entry; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37414245
Proper citation: RRID:Addgene_58324 Copy
Species: Homo sapiens
Genetic Insert: IL36RN
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:4900; Vector Backbone:pCMV6-entry; Vector Types:Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:37414245
Proper citation: RRID:Addgene_58321 Copy
Species: Shewanella oneidensis
Genetic Insert: PepTso
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pWALDOe; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:21131908
Proper citation: RRID:Addgene_58334 Copy
Species: Streptococcus thermophilus
Genetic Insert: PepTSt
Vector Backbone Description: Backbone Size:5600; Vector Backbone:pWALDOd; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22659829
Proper citation: RRID:Addgene_58333 Copy
Species: Homo sapiens
Genetic Insert: drebrin
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23979715
Proper citation: RRID:Addgene_58336 Copy
Species: Arabidopsis thaliana
Genetic Insert: CIB1[1-147]
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4742; Vector Backbone:pmCerulean-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24793453
Proper citation: RRID:Addgene_58367 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2[1-498] (D387A)
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24793453
Proper citation: RRID:Addgene_58369 Copy
Species: Arabidopsis thaliana
Genetic Insert: CRY2[1-498]
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4722; Vector Backbone:pmCherry-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24793453
Proper citation: RRID:Addgene_58368 Copy
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