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Species: Synthetic
Genetic Insert: D2 second generation cameleon (calcium sensor)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4000; Vector Backbone:pBAD; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16720273
Comments: Genetically encoded, ratiometric, calcium biosensor that contains enhanced CFP and citrine. D2 does not bind wild-type calmodulin. It has a Kd1' and Kd2' of 0.8 microM and 28 microM.
See also Palmer & Tsien (2006) Nature Protocols.
Proper citation: RRID:Addgene_37470 Copy
Species: Synthetic
Genetic Insert: PIGA zinc finger L1
Vector Backbone Description: Backbone Marker:Keith Joung lab @ MGH/HMS; Backbone Size:5709; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.
Proper citation: RRID:Addgene_37797 Copy
Species: Synthetic
Genetic Insert: PIGA zinc finger L2
Vector Backbone Description: Backbone Marker:Keith Joung lab @ MGH/HMS; Backbone Size:5709; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.
Proper citation: RRID:Addgene_37798 Copy
Species: Synthetic
Genetic Insert: PIGA zinc finger R1
Vector Backbone Description: Backbone Marker:Keith Joung lab @ MGH/HMS; Backbone Size:5709; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.
Proper citation: RRID:Addgene_37799 Copy
Species: Synthetic
Genetic Insert: PIGA zinc finger R2
Vector Backbone Description: Backbone Marker:Keith Joung lab @ MGH/HMS; Backbone Size:5709; Vector Backbone:pST1374; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19540188
Comments: Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.
Proper citation: RRID:Addgene_37800 Copy
Species: Synthetic
Genetic Insert: MBP (Maltose binding protein)
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5200; Vector Backbone:FastBac-Dual; Vector Types:Insect Expression, Baculovirus expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_38008 Copy
Species: Synthetic
Genetic Insert: Avian sarcoma and leukemia virus receptor 950
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:5604; Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22681690
Proper citation: RRID:Addgene_38044 Copy
Species: Synthetic
Genetic Insert: GoldyTALEN
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pBSK; Vector Types:mRNA transcription vector; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23027955
Comments: Please note that this plasmid can be prone to recombination in bacteria. We recommend storing and propagating the construct in Stbl3 cells and screen 4-6 colonies by sequencing with TAL_F1 (ttggcgtcggcaaacagtgg) primer.
The F1 origin feature in the full plasmid sequence is in reverse compliment orientation. Please view Addgene's quality control sequence for the most accurate information. The flipped orientation does not affect the SacI site necessary to linearize the plasmid.
For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899).
For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson
Proper citation: RRID:Addgene_38142 Copy
Species: Synthetic
Genetic Insert: GoldyTALEN
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pBSK; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23027955
Comments: For further reference, this plasmid was also used in the Bedell et al Nature 2012 publication (PMID 23000899).
For more information on Carlson TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALeffector/goldengate/add-ons/#carlson
Proper citation: RRID:Addgene_38143 Copy
Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Vector Backbone:Phi80; Vector Types:Synthetic Biology, Integration vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22615351
Comments: This strain contains Addgene plasmid 38200 chromosomally integrated at the Phi80 (Haldimann et al., J of Bacteriology 2001) site of DH5alphaZ1 strain.
Proper citation: RRID:Addgene_38203 Copy
Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Vector Backbone:Phi80; Vector Types:Synthetic Biology, Integration vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22615351
Comments: This construct has attL and attR sites (LR).
To integrate on your favorite E.coli strain, transform in strain containing the pAH123 plasmid (Haldimann et al., J of Bacteriology 2001). Contains a R6K origin of replication, will only replicate in pir2+ strains.
Proper citation: RRID:Addgene_38200 Copy
Species: Synthetic
Genetic Insert: mKate2
Vector Backbone Description: Vector Backbone:Phi80; Vector Types:Synthetic Biology, Integration vector; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22615351
Comments: This strain contains Addgene plasmid 38170 chromosomally integrated at the Phi80 (Haldimann et al., J of Bacteriology 2001) site of DH5alphaZ1 strain.
Proper citation: RRID:Addgene_38202 Copy
Species: Synthetic
Genetic Insert: E2-Crimson
Vector Backbone Description: Backbone Marker:Glick Lab; Backbone Size:4313; Vector Backbone:YIPlac204TKC-DsRed-HDEL (Addgene Plasmid #21770); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19658435
Comments: Vector for targeting E2-Crimson to the ER in yeast cells.
Linearize with EcoRV to integrate at TRP
Substitutions in E2-Crimson relative to DsRed-Express2 are: E32V, Q66F, V71A, V73I, K83L, L85Q, F118L, L150N, I161N, K163M, V175C, Y193H, S197Y
Proper citation: RRID:Addgene_38771 Copy
Species: Synthetic
Genetic Insert: E2-Crimson
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5603; Vector Backbone:pEF/myc/ER; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19658435
Comments: Vector for targeting E2-Crimson to the ER in mammalian cells.
Substitutions in E2-Crimson relative to DsRed-Express2 are: E32V, Q66F, V71A, V73I, K83L, L85Q, F118L, L150N, I161N, K163M, V175C, Y193H, S197Y
IMPORTANT NOTE: There is an 82-bp intron after the ER signal sequence. The signal sequence will be in frame with the final product.
Proper citation: RRID:Addgene_38770 Copy
Species: Synthetic
Genetic Insert: EGFP , EGFP sgRNA
Vector Backbone Description: Vector Backbone:pRDA_221; Vector Types:Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_169142 Copy
Species: Synthetic
Genetic Insert: 3xFlag-6His-nsp15
Vector Backbone Description: Vector Backbone:pBIG1a (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198324
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169166 Copy
Species: Synthetic
Genetic Insert: ReaChR-Citrine
Vector Backbone Description: Backbone Marker:Chi-Bin Chien lab; Backbone Size:7796; Vector Backbone:pDestTol2CG2; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32516597
Proper citation: RRID:Addgene_169046 Copy
Species: Synthetic
Genetic Insert: 14His-SUMO-nsp10-GGSGGS-nsp14
Vector Backbone Description: Vector Backbone:pK27SUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198326
Proper citation: RRID:Addgene_169161 Copy
Species: Synthetic
Genetic Insert: nsp10-6His-3xFlag
Vector Backbone Description: Vector Backbone:pBIG1b (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Spectinomycin
Defining Citation: PMID:34198326
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169162 Copy
Species: Synthetic
Genetic Insert: nsp14/nsp10-6His-3xFlag
Vector Backbone Description: Vector Backbone:pBIG2ab (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34198326
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169164 Copy
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