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Vector Backbone Description: Backbone Marker:Mo Bi Tec; Vector Backbone:pET01; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18930792
Proper citation: RRID:Addgene_32484 Copy
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Comments: The full plasmid sequence was assembled and may contain minor discrepancies, which should not affect the function of the plasmid.
Proper citation: RRID:Addgene_32684 Copy
Vector Backbone Description: Backbone Size:8453; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Proper citation: RRID:Addgene_32682 Copy
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pLKO.1 puro; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21418658
Comments: The full plasmid sequence was digitally assembled and may contain minor discrepancies, which should not effect the function of the plasmid.
Proper citation: RRID:Addgene_32685 Copy
Vector Backbone Description: Backbone Size:4427; Vector Backbone:pACAGW-ChR2-Venus-AAV; Vector Types:AAV, aav; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21772812
Proper citation: RRID:Addgene_32671 Copy
Genetic Insert: ccdB
Vector Backbone Description: Vector Backbone:pJFRC7; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Please see http://www.everyvector.com/sequences/show_public/13276 for additional plasmid map.
Proper citation: RRID:Addgene_41439 Copy
Vector Backbone Description: Backbone Size:9260; Vector Backbone:pLKO; Vector Types:Mammalian Expression, Lentiviral, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Plasmid for constitutive lentiviral expression.
Alternate plasmid names:
pLX305,
SV40-puro; PGK-gateway-no tag
Proper citation: RRID:Addgene_41390 Copy
Vector Backbone Description: Backbone Size:9354; Vector Backbone:pCW57.1; Vector Types:Mammalian Expression, Lentiviral, Gateway Destination vector, Doxycycline inducible; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The plasmid expresses rtTA-VP16-2A-puro from the PGK promoter, so it can be used as an 'all-in-one' dox on system.
Alternate plasid names:
pLIX_401
TRE-gateway; PGK-rtTA-2A-puro
Proper citation: RRID:Addgene_41393 Copy
Vector Backbone Description: Backbone Size:7556; Vector Backbone:MSCV; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18988847
Proper citation: RRID:Addgene_41941 Copy
Genetic Insert: YFP
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42153 Copy
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42154 Copy
Genetic Insert: mTFP1
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42155 Copy
Genetic Insert: CFP
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42151 Copy
Species: Caenorhabditis elegans
Genetic Insert: mTFP1::[(G4S)3]::C-TAP Tag
Vector Backbone Description: Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:23281894
Comments: Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc.
Proper citation: RRID:Addgene_42166 Copy
Genetic Insert: TALE-TF(VP64)-BB
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Adeno-associated Virus (); Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22828628
Comments: This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.
IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece.
Proper citation: RRID:Addgene_42581 Copy
Genetic Insert: TALEN-FokI(WT)-BB
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:Mammalian Expression, AAV, Adeno-associated Virus (); Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22828628
Comments: This is a new collection of constructs described in Cong et al. Nature Communications and also an updated TALE Tool Box containing Adeno-associated Virus (AAV) TALE Backbone constructs that allow packaging of TAL transcription factors (TALE-TF) and TALE nucleases (TALEN) into AAV. All constructs are from Feng Zhang lab at Broad Institute/MIT.
IMPORTANT: please note when using the new AAV TALE Backbones, the last 0.5 (half) repeat is not encoded in the backbone vector but instead need to be supplemented as a separate piece.
Proper citation: RRID:Addgene_42588 Copy
Vector Backbone Description: Vector Backbone:NA; Vector Types:Zebrafish enhancer assay vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:22442009
Comments: Gateway A cassette inserted between XhoI and Bglll. Please note that the cassette is not included within the depositor's sequence.
Proper citation: RRID:Addgene_37846 Copy
Vector Backbone Description: Backbone Marker:Connie Cepko lab (Harvard Medical School, Boston, MA); Backbone Size:7349; Vector Backbone:pEF-GFP (Addgene Plasmid #11154); Vector Types:Mammalian Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:18754012
Comments: Alternate plasmid name: pEF1α-RfC.1-IRES-GFP
To construct this vector, the depositing lab used EcoRI and BsrGI to replace the EGFP in pEF–GFP (Addgene plasmid 11154) with the IRES-GFP fragment from plasmid pBMN-I-GFP (Addgene plasmid 1736), creating intermediate vector pEF1α-IRES-GFP.
Plasmid pEF1α-IRES-GFP was then converted to a Gateway-enabled destination vector with the Gateway Vector Conversion System (Invitrogen) by digesting with EcoRI, blunting the ends, and inserting Reading Frame Cassette C (RfC.1) gateway cassette.
Proper citation: RRID:Addgene_45443 Copy
Vector Backbone Description: Backbone Size:9500; Vector Backbone:pCWX-DEST; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21590398
Comments: More information can be found at http://lentilab.unige.ch/ .
Proper citation: RRID:Addgene_45957 Copy
Vector Backbone Description: Backbone Size:10921; Vector Backbone:pCLX-pTF-DEST; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:21761975
Comments: More information can be found at http://medweb2.unige.ch/salmon/lentilab/lentivectors.html
Proper citation: RRID:Addgene_45952 Copy
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