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Species: Saccharomyces cerevisiae
Genetic Insert: CDC42pr-GFP1-10
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6-NATMX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27831485
Proper citation: RRID:Addgene_86420 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: CDC42pr-KAR2SS-GFP1-10
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pFA6-NATMX; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27831485
Proper citation: RRID:Addgene_86421 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VMA intein
Vector Backbone Description: Vector Backbone:pMAL-c5X; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28186733
Comments: TGTCCTACTCAGGAGAGCGTTCAC to sequence VMA C-term; and ACCCATGACCTTATTACCAACCTC to sequence the insert between MBP C-term and VMA
Proper citation: RRID:Addgene_86590 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1 upstream of HIS3
Vector Backbone Description: Backbone Size:0; Vector Backbone:pUC119; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:10793159
Comments: UB1 fragment (SphI-SacI) from pRB306 inserted into SphI-SacI-cut polylinker of pUC119. Then, SalI-KpnI (partial) fragment from pJJ217 containing the entire HIS3 gene and flanking regions, inserted into XhoI-KpnI vector fragment; HIS3 is in the same orientation that TUB1 was; note that this disruption construct removes the first 763 bp of the 1580-bp ORF downstream of TUB1 (YML086c). For information on pUC119, search for pUC119 in Addgene's vector db.
Proper citation: RRID:Addgene_8670 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1
Vector Backbone Description: Backbone Marker:Botstein et al. Gene. 1979 8(1):17-24.; Backbone Size:0; Vector Backbone:YEp21; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:3540600
Comments: A TUB1 fragment was ligated from the SphI site (1.1 kilobases before the start codon) to a BglII site (0.5 kb beyond the stop codon) in place of the small SphI-to-BamHI (??) fragment of YEp21. For more information on YEp21, search for YEp21 in Addgene's vector db.
Proper citation: RRID:Addgene_8668 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1-D252A under GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11739794
Comments: PCRed TUB1-D252A products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
Proper citation: RRID:Addgene_8673 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1-828 under GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11739794
Comments: TUB1-828 was from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903. It was PCRed, and the PCR products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
Please note that this construct is missing the first 8 amino acids of the TUB1 ORF. This deletion has no effect on the plasmid function as the 2 point mutations make this a dominant lethal allele.
Proper citation: RRID:Addgene_8672 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: GFP::TUB1-828 under GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS408; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11739794
Comments: PCR amplified TUB1-828 DNA were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS408. pTS408 was constructed by PCR amplifying GFP with BamHI and BglII sites and by inserting this into the BamHI site of pTS210, creating a plasmid with the polylinker downstream of GFP to create NH2-terminal GFP fusions.The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
Proper citation: RRID:Addgene_8678 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: tub1-827 under GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11739794
Comments: tub1-827 was from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903. It was PCRed, and the PCR products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
Proper citation: RRID:Addgene_8676 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: TUB1-820 under GAL1 promoter
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTS210; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11739794
Comments: PCRed TUB1-820 (from Richards et al 2000 Mol. Biol. Cell 11, 1887-1903) products were digested with BamHI and SpeI and ligated to the BamHI and XbaI sites of pTS210. The vector plasmid pTS210, a YCp50-based construct, contained the GAL1/GAL10 promoter, a short polylinker (BamHI, HindIII, XbaI), and the ACT1 transcriptional terminator.
Proper citation: RRID:Addgene_8675 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87283 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87284 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87282 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87285 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87272 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87273 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87270 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87271 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87277 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: HSH155
Vector Backbone Description: Backbone Marker:ATCC; Backbone Size:4788; Vector Backbone:pRS414; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:28062854
Proper citation: RRID:Addgene_87274 Copy
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