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Species: Drosophila melanogaster
Genetic Insert: PolIII-Renilla control reporter
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:3320; Vector Backbone:pRL-null; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16311596
Comments: Perrimon Lab plasmid #414
The PolIII–Renilla luciferase control construct was generated by PCR amplification of a fragment (base pairs 43,224–43,389 of scaffold AE003823) of the promoter of the D. melanogaster RNA PolIII 128 subunit (RpIII128) with a 5' BglII and a 3' SpeI site and ligation of this fragment into the BglII and SpeI sites of pRL-null.
Proper citation: RRID:Addgene_37380 Copy
Genetic Insert: Cre Shine
Vector Backbone Description: Backbone Marker:Invitrogene; Backbone Size:5060; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_37404 Copy
Species: Drosophila melanogaster
Genetic Insert: tout-velu
Vector Backbone Description: Vector Backbone:pNB40; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9665133
Comments: Perrimon Lab plasmid #179
Proper citation: RRID:Addgene_37401 Copy
Species: Homo sapiens
Genetic Insert: EEF1D
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1+; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21936567
Proper citation: RRID:Addgene_37364 Copy
Species: Homo sapiens
Genetic Insert: npc1l1
Vector Backbone Description: Backbone Marker:invitrogen; Backbone Size:5000; Vector Backbone:pFASTBAC; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21525977
Comments: There is a TEV cleavage site downstream of His tag.
Proper citation: RRID:Addgene_37366 Copy
Species: Homo sapiens
Genetic Insert: CK2beta 6KR
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17681943
Proper citation: RRID:Addgene_37361 Copy
Species: Homo sapiens
Genetic Insert: CK2α V66A/I174A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5542; Vector Backbone:pRC/CMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18258654
Comments: there is a 3bp deletion (AGG) in the MCS 131.
Proper citation: RRID:Addgene_37362 Copy
Species: Synthetic
Genetic Insert: NLS-mCherry
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594
Proper citation: RRID:Addgene_37354 Copy
Species: Synthetic
Genetic Insert: membrane TdTomato
Vector Backbone Description: Backbone Marker:clontech; Backbone Size:6000; Vector Backbone:pQC; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21397594
Comments: *palmitoylation sequence: 5'-atgctgtgctgtatgagaagaaccaaacaggttgaaaagaatgatgaggaccaaaagatc-3'
Proper citation: RRID:Addgene_37351 Copy
Species: Pea
Genetic Insert: Connexin43 fused to pea APEX
Vector Backbone Description: Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23086203
Proper citation: RRID:Addgene_44439 Copy
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pBabe; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15644491
Proper citation: RRID:Addgene_44436 Copy
Species: Vibrio cholerae
Genetic Insert: AphA
Vector Backbone Description: Backbone Marker:AATC; Backbone Size:8807; Vector Backbone:pMMB66EH; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23919997
Proper citation: RRID:Addgene_44392 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: yHse1-UIM (150-190)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST-parallel.1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20150893
Proper citation: RRID:Addgene_44429 Copy
Species: Homo sapiens
Genetic Insert: mitoDsRed
Vector Backbone Description: Backbone Size:6727; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314018
Comments: Limitations of allotopic expression of mitochondrial genes in mammalian cells. Oca-Cossio J, Kenyon L, Hao H, Moraes CT.
Genetics. 2003 Oct;165(2):707-20. PMID: 14573482
Proper citation: RRID:Addgene_44386 Copy
Species: Homo sapiens
Genetic Insert: mitoGFP
Vector Backbone Description: Backbone Size:6727; Vector Backbone:pLV; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23314018
Proper citation: RRID:Addgene_44385 Copy
Species: Vibrio cholerae
Genetic Insert: toxTpro
Vector Backbone Description: Vector Backbone:pBH6119; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23919997
Comments: The toxT promoter drives expression of a stable form of GFPmut3. Please note that although this plasmid can be propagated in E. coli, it is a Vibrio cholerae vector and should be used in a Vibrio strain for experiments.
Proper citation: RRID:Addgene_44388 Copy
Species: Homo sapiens
Genetic Insert: TOM1-VHS (1-152)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20150893
Proper citation: RRID:Addgene_44423 Copy
Species: Homo sapiens
Genetic Insert: HRS-VHS (1-143)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20150893
Proper citation: RRID:Addgene_44416 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: Vps27-VHS (2-150)
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20150893
Proper citation: RRID:Addgene_44417 Copy
Species: Homo sapiens
Genetic Insert: GGA2-VHS
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pGST1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:20150893
Proper citation: RRID:Addgene_44419 Copy
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