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Genetic Insert: ccdb
Vector Backbone Description: Vector Backbone:I-SceI; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:32709891
Proper citation: RRID:Addgene_118628 Copy
Genetic Insert: cat, sacB
Vector Backbone Description: Backbone Size:0; Vector Backbone:pTP809; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:10767554
Comments: pKM154 is a derivative of pBR322 that had sequence between the EcoR1 site and NdeI site removed, with a NotI site inserted (pTP809).
Proper citation: RRID:Addgene_13036 Copy
Genetic Insert: EGFP
Vector Backbone Description: Backbone Size:5778; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_13071 Copy
Genetic Insert: 6x Myc Tag
Vector Backbone Description: Backbone Size:5818; Vector Backbone:na; Vector Types:; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:17948311
Proper citation: RRID:Addgene_13070 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:10365; Vector Backbone:pGAN049; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is one of the empty dual yTRAP sensor plasmids. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK247. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with pSK260.
Proper citation: RRID:Addgene_131148 Copy
Vector Backbone Description: Backbone Marker:Szilvia Kiriakov; Backbone Size:10377; Vector Backbone:pSK247; Vector Types:Yeast Expression, Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty dual yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pSK234. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the LEU2 locus, and can be selected for using 100 ug/ml G418. It can be used in combination with an expression clone resulting from LR reaction using pGAN147 basic yTRAP sensor.
Proper citation: RRID:Addgene_131149 Copy
Vector Backbone Description: Backbone Marker:Gregory Newby; Backbone Size:11381; Vector Backbone:pGAN147; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:29056345
Comments: This is the empty reverse yTRAP sensor plasmid. To sense aggregation of your favorite protein, clone the coding sequence into a pENTR clone, then perform LR reaction with pGAN230. The resulting expression clone should be linearized with NotI-HF enzyme prior to integration into the yeast genome. The sensor integrates into the HO locus, and can be selected for using nourseothricin. To achieve dual color reporting, combine with pSK221.
Proper citation: RRID:Addgene_131122 Copy
Vector Backbone Description: Backbone Size:11698; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, Other, Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134975 Copy
Vector Backbone Description: Backbone Size:11095; Vector Backbone:pCR8/GW/TOPO; Vector Types:Mammalian Expression, Other, Gateway cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134976 Copy
Vector Backbone Description: Backbone Size:4723; Vector Backbone:pX330; Vector Types:Mammalian Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31541098
Proper citation: RRID:Addgene_134978 Copy
Genetic Insert: Hygromycin resistance
Vector Backbone Description: Backbone Size:7654; Vector Backbone:pCFJ201; Vector Types:Worm Expression, CRISPR; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:31378567
Proper citation: RRID:Addgene_135096 Copy
Vector Backbone Description: Vector Backbone:unknown; Vector Types:E.coli/S.aureus shuttle vector; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16051359
Proper citation: RRID:Addgene_133446 Copy
Genetic Insert: LacZ reporter
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:9700; Vector Backbone:pLacZi; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:16777607
Comments: Please note mutation R15H in ccdB was found during Addgene's quality control process. The plasmid should function as reported in the associated publication.
Please note that the ccdB cassette in the author's map (click on 'View map') is shown in the reverse orientation.
Y1H Destination vector that can be used in conventional Gateway LR cloning reactions. This vector contains a Gateway cassette with AttR4 and AttL1 recombination sites and a LacZ reporter gene. For more information and protocols, see Deplancke et al. 2004 Genome Res 14(10B):2093 and Deplancke et al. 2006 CSH Protocols.
Proper citation: RRID:Addgene_13350 Copy
Vector Backbone Description: Backbone Size:9866; Vector Backbone:pAWH; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:30217970
Proper citation: RRID:Addgene_133894 Copy
Vector Backbone Description: Vector Backbone:pmCellFree_KA3; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Ampicillin
Proper citation: RRID:Addgene_169408 Copy
Vector Backbone Description: Vector Backbone:pCG150 - Addgene plasmid 17247; Vector Types:Worm Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:34791215
Comments: Please visit https://doi.org/10.1101/2020.11.25.398784 for bioRxiv preprint.
Proper citation: RRID:Addgene_169693 Copy
Vector Backbone Description: Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161880 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161883 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161887 Copy
Vector Backbone Description: Vector Backbone:pFUGW; Vector Types:Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Defining Citation: PMID:24395366
Proper citation: RRID:Addgene_161888 Copy
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